Susceptibility of Enterobacteriaceae to β-lactam agents and fluoroquinolones: a 3-year survey in France

Sirot, J.; Nicolas–Chanoine, Marie–Hélène; Chardón, H.; Avril, J; Cattöen, C.; Croix, J.C.; Dabernat, H.; Fosse, T.; Ghnassia, J. C.; Lecaillon, E.; Marmonier, Aurélie; Roussel-Delvallez, M.; Soussy, C J; Trevoux, A.; Vandenesch, François; Dib, Cristina Corsi; Moniot-Ville, N; Rezvani, Y.

doi:10.1046/j.1469-0691.2002.00393.x

Cited by 25 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased incidence of bacterial resistance to antimicrobial agents in both nosocomial and community settings requires careful thought. A French prospective study with 320 E. coli strains isolated from urinary tract infections between 1998 and 1999 showed that the prevalence of resistance to amoxicillin was 46.1% (31). Gupta et al showed that the prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in women is also increasing (14).…”

Section: Discussionmentioning

confidence: 99%

Effects of Intrapartum Penicillin Prophylaxis on Intestinal Bacterial Colonization in Infants

et al. 2004

View full text Add to dashboard Cite

Early-onset group B streptococcal (GBS) infections remain a leading cause of morbidity and mortality in infants. To prevent the vertical transmission of GBS and neonatal GBS infection, guidelines recommend intrapartum penicillin or amoxicillin prophylaxis. This intrapartum antibiotic prophylaxis (IAP) is suspected to favor colonization by antibiotic-resistant bacteria. However, the effects of this prophylaxis on the patterns of acquisition of gastrointestinal bacterial flora in infants have never been studied. We collected stool samples from 3-day-old infants born to mothers who received intrapartum amoxicillin (antibiotic-exposed group; n ‫؍‬ 25) and to untreated mothers (non-antibiotic-exposed group; n ‫؍‬ 25). The groups were matched for factors known to affect intestinal microbial colonization: gestational age, type of delivery, and type of feeding. Qualitative and quantitative differential analyses of the bacterial flora in stool samples were performed. Similar numbers of infants in the non-antibiotic-exposed and antibiotic-exposed groups were colonized by aerobic bacteria and amoxicillin-resistant enterobacteria (75 and 77%, respectively) (P ‫؍‬ 0.79). In contrast, significantly fewer infants in the antibiotic-exposed group than in the non-antibiotic-exposed group were colonized by anaerobic bacteria, especially Clostridium (12 and 40%, respectively) (P < 0.05). Regarding intestinal bacterial colonization, the differences between antibiotic-exposed and non-antibiotic-exposed infants were remarkably few. The only statistically significant effect was the reduced initial bacterial colonization by Clostridium in the antibiotic-exposed group. In our study, the use of IAP did not favor colonization by ␤-lactam-resistant bacteria. However, further evaluations are required to highlight the potential risks of the widespread use of antibiotics to prevent early-onset GBS infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Intrapartum Penicillin Prophylaxis on Intestinal Bacterial Colonization in Infants

et al. 2004

View full text Add to dashboard Cite

show abstract

“…While ESBLs were initially found mainly in Klebsiella pneumoniae and Escherichia coli, an increasing variety of gram-negative species have been shown to harbor such resistance mechanisms (7,8). In recent years, ESBLs have become more prevalent in AmpC-producing species (1,16). In spite of the worldwide use of ␤-lactam antimicrobial agents, which is considered the main reason for the emergence of ESBLs (2), the distributions of ESBLs are far from uniform.…”

mentioning

confidence: 99%

Widely Distributed and Predominant CTX-M Extended-Spectrum β-Lactamases in Amsterdam, The Netherlands

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Extended-spectrum beta-lactamases (ESBLs) are important enzymes that cause resistance to extended-spectrum cephalosporins in the widespread pathogens Klebsiella, Escherichia, Proteus, and Enterobacter (1,27,28); rarely in other members of the family Enterobacteriaceae; and sporadically in nonfermentating gram-negative bacilli such as Pseudomonas and Acinetobacter spp. (38,39).…”

mentioning

confidence: 99%

Detection and Genotyping of SHV β-Lactamase Variants by Mass Spectrometry after Base-Specific Cleavage of In Vitro-Generated RNA Transcripts

Stürenburg

Storm²,

Sobottka

et al. 2006

J Clin Microbiol

View full text Add to dashboard Cite

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after base-specific cleavage of PCR-amplified and in vitro-transcribed bla SHV genes was used for the identification and genotyping of SHV ␤-lactamases. For evaluation, bla SHV stretches of 21 clinical Enterobacteriaceae isolates were PCR amplified using T7 promoter-tagged forward and reverse primers, respectively. In vitro transcripts were generated with T7 RNA and DNA polymerase in the presence of modified analogues replacing either CTP or UTP. Using RNase A, the in vitro transcripts were base-specifically cleaved at every "T" or "C" position. Resulting cleavage products were analyzed by MALDI-TOF MS, generating a characteristic signal pattern based on the fragment masses. All 21 individual SHV genes were identified unambiguously using reference sequences, and the results were in perfect concordance with those obtained by fluorescent dideoxy sequencing, which represents the current standard method. As multiple point mutations can be detected in a single assay and newly emerged mutations which are not yet described in public databases can be identified too, MALDI-TOF MS appears to be an ideal tool for analysis of sequence polymorphisms in resistance-associated gene loci.

show abstract

Susceptibility of Enterobacteriaceae to β-lactam agents and fluoroquinolones: a 3-year survey in France

Abstract: Over the 3-year study period beta-lactams and fluoroquinolones remained highly active against Enterobacteriaceae clinical isolates, with the exception of E. aerogenes, probably as a result of the dissemination of multiresistant clones in French hospitals.

Cited by 25 publications

References 20 publications

Effects of Intrapartum Penicillin Prophylaxis on Intestinal Bacterial Colonization in Infants

Effects of Intrapartum Penicillin Prophylaxis on Intestinal Bacterial Colonization in Infants

Widely Distributed and Predominant CTX-M Extended-Spectrum β-Lactamases in Amsterdam, The Netherlands

Detection and Genotyping of SHV β-Lactamase Variants by Mass Spectrometry after Base-Specific Cleavage of In Vitro-Generated RNA Transcripts

Contact Info

Product

Resources

About