Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study

Zanin, Iriana Carla Junqueira; Gonçalves, Reginaldo Bruno; Brugnera, Aldo; Hope, Christopher K.; Pratten, Jonathan

doi:10.1093/jac/dki232

Cited by 266 publications

(256 citation statements)

References 28 publications

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“…Recently, alternative light sources, such as LED, have been successfully used in PDT. 25,26,44 In the present investigation, LED was used as a light source because of its ability to irradiate larger areas than is possible with collimated laser light. Moreover, LED technology is simpler and has a lower cost than laser.…”

Section: Discussionmentioning

confidence: 99%

“…However, light sources with simpler technology and lower cost than lasers, such as light-emitting diodes (LED), have been successfully applied in PDT. 25,26 In addition, there are different colors of LED light, with radiations covering almost the entire visible electromagnetic spectrum. Investigations with the aim of confirming the effectiveness of antimicrobial PDT in animal models are scarce.…”

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confidence: 99%

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Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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“…The efficacy of PDT with erythrosine in S. mutans has been previously studied 16,17) . Wood et al 16) compared the use of three different photosensitizing agents: erythrosine, Photofrin, and methylene blue at 22 μ M for each dye concentration to photosensitize the S. mutans biofilm, using 400 W tungsten lamp.…”

Section: ⅳ Discussionmentioning

confidence: 99%

“…Previous studies have shown that PDT using erythrosine is capable of killing oral bacteria [15][16][17][18] . Recently, Park et al 15) proved that PDT effect of erythrosine as a photosensitizer and dental halogen curing unit as a light source to the planktonic condition of S. mutans.…”

Section: ⅰ Introductionmentioning

confidence: 99%

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

Jung¹,

Park²,

Lee

et al. 2012

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. The aim of this study was to evaluate the effect of PDT on Streptococcus mutans in planktonic conditions, previously treated with different photosensitive concentrations of erythrosine, using halogen and LED curing unit as a light source. And we compared the effects of PDT on six strains of S. mutans isolated from oral cavity and reference strain.As a result, S. mutans was susceptible to the combination of hand held photopolymerizer (HHP) and erythrosine. The higher concentration of erythrosine in the presence of light irradiation induced greater effects in reduction of viability of S. mutans. Isolated S. mutans showed a significant reduction in bacterial counts of the groups submitted to PDT compared to the control groups. And they appeared to be similar or slightly lower antimicrobial effect compared with reference strain. However, the difference was not significant (p < 0.05).In conclusion, PDT using erythrosine as a photosensitizing agent and HHP as a light source could be an efficient option for diseases caused by S. mutans.

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“…Previous studies have examined the effects of PDT on single-species biofilms using streptococci to model supragingival plaque in conditions associated with onset of dental caries (Zanin et al, 2005(Zanin et al, , 2006 and Streptococcus pyogenes to model a skin biofilm . However, PDT also offers a promising, non-invasive treatment for periodontal disease.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of microcosm subgingival dental plaques to lethal photosensitization

2007

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A B S T R A C TPhotodynamic therapy (PDT) offers potential as a non-invasive treatment of periodontal disease. In this study, microcosm biofilms were grown in vitro under conditions designed to mimic subgingival plaques typically found in patients with periodontitis. To investigate potential PDT modalities, biofilms were exposed to light from a helium/neon laser in conjunction with a photosensitizer, toluidine blue O (TBO), at varying output and concentration, respectively. To determine cytotoxic effects, viability profiling was undertaken on whole biofilms using standard plating methods, and on horizontal cross-sections of biofilms using confocal laser-scanning microscopy (CLSM) in conjunction with a differential viability stain. A light energy dose of 94.5 J in combination with 81.7 µM TBO was found to be optimal, achieving significant kills of over 97%. CLSM enabled visualization of the effects of PDT in three dimensions. Viability profiling of the CLSM images revealed that lethal photosensitization was most effective in the upper layers of biofilm. PDT was found to reduce the viability of subgingivally modelled plaques in vitro by a magnitude similar to that of chlorhexidine digluconate, which is commonly used to treat periodontal disease. The findings of this study indicate that PDT may be an effective alternative to conventional modalities in the treatment of periodontal disease.

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Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study

Abstract: Photodynamic therapy may be a useful approach in the treatment of dental plaque-related diseases.

Cited by 266 publications

References 28 publications

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

Susceptibility of microcosm subgingival dental plaques to lethal photosensitization

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