Swelling behaviours and mechanical properties of silk fibroin–polyurethane composite hydrogels

Huang, Yiping; Zhang, Baoping; Ge-wen, XU; Hao, Wentao

doi:10.1016/j.compscitech.2013.05.007

Cited by 86 publications

(46 citation statements)

References 33 publications

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“…In previous studies, the carrageenan-based hydrogel showed a great swelling ratio (140%~299%) [40]. The equilibrium swelling ratio of silk fibroin/polyurethane composite hydrogel was about 5% [41]. The swelling ratio of a chemically modified chitosan biopolymer is 50%~175% [42].…”

Section: Swelling Performance and Stimulus Responsive Behaviorsmentioning

confidence: 99%

An Environmentally Sensitive Silk Fibroin/Chitosan Hydrogel and Its Drug Release Behaviors

Tang

Zhao

2019

Polymers

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To fabricate environmentally sensitive hydrogels with better biocompatibility, natural materials such as protein and polysaccharide have been widely used. Environmentally sensitive hydrogels can be used as a drug carrier for sustained drug release due to its stimulus responsive performance. The relationship between the internal structure of hydrogels and their drug delivery behaviors remains indeterminate. In this study, environmentally sensitive hydrogels fabricated by blending silk fibroin/chitosan with different mass ratios were successfully prepared using 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS) cross-linking agent. Scanning-electron microscopy (SEM) images showed the microcosmic surface of the gel had a 3-D network-like and interconnected pore structure. The N 2 adsorption-desorption method disclosed the existence of macroporous and mesoporous structures in the internal structure of hydrogels. Data of compression tests showed its good mechanical performance. The swelling performance of hydrogels exhibited stimuli responsiveness at different pH and ion concentration. With the increase of pH and ion concentration, the swelling ratios of hydrogels (silk fibroin (SF)/ chitosan (CS) = 8/2 and 7/3) decreased. Methylene blue (MB) was loaded into the hydrogels to confirm the potential of sustained drug release and pH-responsive behavior. Therefore, due to the porous structure, stable mechanical strength, stimuli responsive swelling performance, and drug release behaviors, the SF/CS composite hydrogels have potential applications in controlled drug release. of hydrogels prepared by chemical cross-linking is relatively stable. The physical properties of hydrogel are similar to the properties of living tissues, such as high water content, rubbery texture, and low interfacial tension, which contribute to its outstanding biocompatibility with the surrounding tissues [6]. The mechanical property, shape, and swelling performance of some hydrogels tend to change with the external conditions, such as temperature, pH value, and ion concentration [7][8][9]. Drugs can be delivered through hydrogels with stimuli responsiveness to pH, magnetism, ultrasound, temperature changes, and electrical effects [8]. The stimuli responsiveness of hydrogel makes it potential material for drug release, which has been proved in previous studies [10][11][12]. Cross-linking mechanism or pore structure of hydrogels may be the cause of stimuli responsiveness, which has not been revealed. In this study, a pH-and ion intensity stimuli-responsive silk fibroin/chitosan hydrogel was prepared by chemical cross-linking. The mechanism of stimulus responsiveness of the hydrogel was also indicated.Silk fibroin (SF) is a kind of natural material with high molecular weight, good biocompatibility, and mechanical strength [13]. Hydrogel made of silk fibroin has broad application prospects in artificial skin, wound dressing, sustained drug release, tissue engineering scaffolds, and so on [14-17]. Chitosan (CS) is a natural ba...

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Section: Swelling Performance and Stimulus Responsive Behaviorsmentioning

confidence: 99%

An Environmentally Sensitive Silk Fibroin/Chitosan Hydrogel and Its Drug Release Behaviors

Tang

Zhao

2019

Polymers

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“…In order to improve performance of such natural polymers and expand the applied range, they has been blended with various of other polymers which include both natural macromolecules like collagen, gelatin, elastin, chitosan, hyaluronic and sodium carboxymethyl cellulose as well as synthetic molecules like polyacrylamide, polystyrene, polyurethane, poly (L-lactide), polyvinylalcohol, poly ethylene glycol11202122232425262728293031. Those blend scaffolds with more superior properties were employed for various tissue engineering applications.…”

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confidence: 99%

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Wang

Shi

et al. 2016

Sci Rep

156

103

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A cytocompatible porous scaffold mimicking the properties of extracellular matrices (ECMs) has great potential in promoting cellular attachment and proliferation for tissue regeneration. A biomimetic scaffold was prepared using silk fibroin (SF)/sodium alginate (SA) in which regular and uniform pore morphology can be formed through a facile freeze-dried method. The scanning electron microscopy (SEM) studies showed the presence of interconnected pores, mostly spread over the entire scaffold with pore diameter around 54~532 μm and porosity 66~94%. With significantly better water stability and high swelling ratios, the blend scaffolds crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) provided sufficient time for the formation of neo-tissue and ECMs during tissue regeneration. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) results confirmed random coil structure and silk I conformation were maintained in the blend scaffolds. What’s more, FI-TR spectra demonstrated crosslinking reactions occurred actually among EDC, SF and SA macromolecules, which kept integrity of the scaffolds under physiological environment. The suitable pore structure and improved equilibrium swelling capacity of this scaffold could imitate biochemical cues of natural skin ECMs for guiding spatial organization and proliferation of cells in vitro, indicating its potential candidate material for soft tissue engineering.

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“…[35][36][37] Compression tests were carried out to obtain stress-strain curves, and to determine the elastic modulus and elastic limit of the silk hydrogels using a mechanical testing apparatus (INSTRON 3365; Instron Corp., Norwood, MA) with a velocity of 1 mm/min at 20°C.…”

Section: Mechanical Properties 2101 Compression Propertiesmentioning

confidence: 99%

Potential of biocompatible regenerated silk fibroin/sodium N-lauroyl sarcosinate hydrogels

Fang

Zhu

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Hydrogels are becoming widely used in biomaterial applications. The available methods for the preparation of these materials are continually growing. The gelation time (GT) of silk protein fibroin is difficult to control by physical methods. The cross-linkers used in available chemical techniques are likely to impact the biocompatibility of the resultant materials. In this paper, we demonstrate that the addition of sodium N-lauroyl sarcosinate (an amino-acid-based surfactant) accelerates the formation of hydrogels from fibroin. GT, turbidity variations, changes of viscoelasticity during the gelation process, and the mechanical properties of the products are measured. The secondary structure was probed by Fourier transform infrared spectroscopy, X-ray diffraction and the morphologies of the products were investigated by scanning electron microscopy. Transformations in the β-sheet content were monitored by the fluorescence of Thioflavine T and circular dichroism measurements. The relationship between the surface tension of sodium N-lauroyl sarcosinate and the GT was also explained. To investigate cell compatibility, fibroblast cells were seeded onto the surface of the hydrogels. The results indicate that the sodium N-lauroyl sarcosinate/fibroin GT can be controlled. This blend-hydrogel demonstrates excellent cell compatibility, good compression strength, and outstanding compression-recovery characteristics. Sodium N-lauroyl sarcosinate/silk fibroin hydrogels containing β-sheets have considerable potential as replacement materials in addressing the tissue defects involved with repair surgery.

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Swelling behaviours and mechanical properties of silk fibroin–polyurethane composite hydrogels

Cited by 86 publications

References 33 publications

An Environmentally Sensitive Silk Fibroin/Chitosan Hydrogel and Its Drug Release Behaviors

An Environmentally Sensitive Silk Fibroin/Chitosan Hydrogel and Its Drug Release Behaviors

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Potential of biocompatible regenerated silk fibroin/sodium N-lauroyl sarcosinate hydrogels

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