2021
DOI: 10.1038/s41467-021-25024-5
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Switching at the ribosome: riboswitches need rProteins as modulators to regulate translation

Abstract: Translational riboswitches are cis-acting RNA regulators that modulate the expression of genes during translation initiation. Their mechanism is considered as an RNA-only gene-regulatory system inducing a ligand-dependent shift of the population of functional ON- and OFF-states. The interaction of riboswitches with the translation machinery remained unexplored. For the adenine-sensing riboswitch from Vibrio vulnificus we show that ligand binding alone is not sufficient for switching to a translational ON-state… Show more

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Cited by 19 publications
(37 citation statements)
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“…Through iterative optimization, we have devised the pCrepusculo setup in which PAL associates with a small RNA hairpin near the ribosome-binding site of target genes and thus represses their expression under blue light by around 8-to 30-fold depending on temperature. While the dynamic range is less than in certain of the transcriptional optogenetic approaches 25,27,46,47 , it compares to or exceeds the regulatory effect realized for the control of bacterial translation initiation by ligandresponsive riboswitches, e.g., references [51][52][53] .…”
Section: Discussionmentioning
confidence: 94%
“…Through iterative optimization, we have devised the pCrepusculo setup in which PAL associates with a small RNA hairpin near the ribosome-binding site of target genes and thus represses their expression under blue light by around 8-to 30-fold depending on temperature. While the dynamic range is less than in certain of the transcriptional optogenetic approaches 25,27,46,47 , it compares to or exceeds the regulatory effect realized for the control of bacterial translation initiation by ligandresponsive riboswitches, e.g., references [51][52][53] .…”
Section: Discussionmentioning
confidence: 94%
“…Initially thought to function exclusively through ligandinduced alternative folding, riboswitches employ diverse mechanisms including transacting regulation (41), mRNA degradation (42), internal transesterification (43), and chaperone-assisted folding (44). The direct binding of the tRNA-like yjdF aptamer domain to intact ribosomes now reveals another mechanism of gene activation by riboswitches (and potentially other noncoding RNAs).…”
Section: Discussionmentioning
confidence: 99%
“…RNA thermometers can be coupled to riboswitches, which bind and sense small molecules, and this combination permits the sensing of small molecules at different temperatures. However, some riboswitches, for example the add riboswitch, which senses both adenine and temperature, requires the interaction with a specific protein subunit of the ribosome, which may limit the transmission between different species [129] . Additional engineering of RNA sequence may be required to facilitate their use in different species.…”
Section: Biotechnological Applications Of Temperature Controlmentioning
confidence: 99%