Syk and Lyn phosphorylation induced by FcγRI and FγRII crosslinking is determined by the differentiation state of U-937 monocytic cells

Hallal-Calleros, Claudia; Agramonte-Hevia, José; Garay-Canales, Claudia; Oliver, Janet M.; Guerra-Araiza, Cristhian; Heras, David; Soto‐Cruz, Isabel; Ortega, Enrique

doi:10.1016/j.imlet.2005.02.010

Cited by 7 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, in preliminary experiments to determine the optimal time-point to measure Syk activation after CD13 cross-linking, we found that CD13 aggregation induces a rapid phosphorylation of Syk that peaks at 40 seconds and starts to drop soon after. In contrast, FcgRI-induced phosphorylation of Syk peaks after 60 seconds of receptor aggregation, and the phosphorylation is maintained for several minutes [42]. Together, these data indicate that CD13 aggregation induces phosphorylation of Syk and that CD13-mediated phagocytosis in macrophages is partially dependent on Syk activation.…”

Section: Syk Activation Is Involved In Cd13-mediated Phagocytosismentioning

confidence: 74%

CD13 mediates phagocytosis in human monocytic cells

Licona‐Limón

Garay-Canales

Muñoz-Paleta

et al. 2015

Journal of Leukocyte Biology

Self Cite

View full text Add to dashboard Cite

CD13 is a membrane-bound ectopeptidase, highly expressed on monocytes, macrophages, and dendritic cells. CD13 is involved in diverse functions, including degradation of peptide mediators, cellular adhesion, migration, viral endocytosis, signaling, and positive modulation of phagocytosis mediated by FcγRs and other phagocytic receptors. In this work, we explored whether besides acting as an accessory receptor, CD13 by itself is a primary phagocytic receptor. We found that hCD13 mediates efficient phagocytosis of large particles (erythrocytes) modified so as to interact with the cell only through CD13 in human macrophages and THP-1 monocytic cells. The extent of this phagocytosis is comparable with the phagocytosis mediated through the canonical phagocytic receptor FcγRI. Furthermore, we demonstrated that hCD13 expression in the nonphagocytic cell line HEK293 is sufficient to enable these cells to internalize particles bound through hCD13. CD13-mediated phagocytosis is independent of other phagocytic receptors, as it occurs in the absence of FcγRs, CR3, and most phagocytic receptors. Phagocytosis through CD13 is independent of its enzymatic activity but is dependent on actin rearrangement and activation of PI3K and is partially dependent on Syk activation. Moreover, the cross-linking of CD13 with antibodies rapidly induced pSyk in human macrophages. Finally, we observed that antibody-mediated cross-linking of hCD13, expressed in the murine macrophage-like J774 cell line, induces production of ROS. These results demonstrate that CD13 is a fully competent phagocytic receptor capable of mediating internalization of large particles.

show abstract

Section: Syk Activation Is Involved In Cd13-mediated Phagocytosismentioning

confidence: 74%

CD13 mediates phagocytosis in human monocytic cells

Licona‐Limón

Garay-Canales

Muñoz-Paleta

et al. 2015

Journal of Leukocyte Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…One hypothesis to explain this result would be that the opsonized particles activate the macrophages and that this activation somehow protects them from the toxic effects of silica. When Fc receptors are crosslinked by binding to opsonized particles, Syk and Src tyrosine kinases are phosphorylated and the cell becomes activated leading to an oxidative burst and expression of TNF and MIP-2 (42,43). To test this hypothesis, cells were activated with antibodycoated latex beads and then exposed to silica.…”

Section: Discussionmentioning

confidence: 99%

The Phagocytosis of Crystalline Silica Particles by Macrophages

Gilberti

Joshi²,

Knecht³

2008

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

Silicosis is a chronic lung disease induced by the inhalation of crystalline silica. Exposure of cultured macrophages to crystalline silica leads to cell death; however, the mechanism of cell-particle interaction, the fate of particles, and the cause of death are unknown. Time-lapse imaging shows that mouse macrophages avidly bind particles that settle onto the cell surface and that cells also extend protrusions to capture distant particles. Using confocal optical sectioning, silica particles were shown to be present within the cytoplasmic volume of live cells. In addition, electron microscopy and elemental analysis showed silica in internal cellular sections. To further examine the phagocytosis process, the kinetics of particle uptake was quantified using an assay in which cells were exposed to ovalbumin (OVA)-coated particles, and an anti-OVA antibody was used to distinguish surface-bound from internalized particles. Fc receptor-mediated uptake of antibody-coated silica particles was nearly complete within 5 minutes. In contrast, no OVA-coated particles were internalized at this time. After 30 minutes, 30% of bound silica was internalized and uptake continued slowly thereafter. OVA-coated latex beads, regardless of surface charge, were internalized at a similarly slow rate. These results demonstrate that macrophages internalize silica and that nonopsonized phagocytosis occurs by a temporally, and possibly mechanistically, distinct pathway from Fc receptor-mediated phagocytosis. Eighty percent of macrophages die within 12 hours of silica exposure. Neither OVA coating nor tetramethylrhodamine isothiocyanate labeling has any effect on cell death. Interestingly, antibody coating dramatically reduces silica toxicity. We hypothesize that the route of particle entry and subsequent phagosome trafficking affects the toxicity of internalized particles.

show abstract

“…The cells were observed using a confocal laser scanning microscopy system (Leica, TCS‐SP2, Wetzlar, Germany) using a λ = 488 nm Argon‐Crypton laser for FITC. The monocytic cell line U‐937, that expresses all members of FcγRs on its membrane (36, 37), was used as control for the entire experimental procedure.…”

Section: Methodsmentioning

confidence: 99%