Synergetic effect of functional cadmium&amp;ndash;tellurium quantum dots conjugated with gambogic acid for HepG2 cell-labeling and proliferation inhibition

Xu, Peipei; Li, Jingyuan; Shi, Lixin; Selke, Matthias; Chen, Baoan; Wang, Xuemei

doi:10.2147/ijn.s51622

Cited by 30 publications

(17 citation statements)

References 27 publications

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“…12 Among them, nanocarriers showed promising potential in minimizing P-gp-mediated MDR by extending the circulation time of drugs in vivo, delivering large amount of drugs to the targeting tissue, and releasing a high concentration of drugs inside the cells after they translocate on the cell membrane. [13][14][15] In our previous report, gambogic acid (GA)-loaded cysteamine-modified cadmium tellurium quantum dots (GA-Cys-CdTe QDs) showed promising ability to improve drug accumulation in K562 cells and minimize MDR of the drug-resistant K562/A02 cells. 16 GA not only shows significant anticancer activity in vitro and in vivo, but also is an attractive agent for the chemosensitization of cancer cells.…”

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confidence: 99%

Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo

Zhou¹,

Wang²,

Chen³

et al. 2016

IJN

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To minimize the side effects and the multidrug resistance (MDR) arising from daunorubicin (DNR) treatment of malignant lymphoma, a chemotherapy formulation of cysteamine-modified cadmium tellurium (Cys-CdTe) quantum dots coloaded with DNR and gambogic acid (GA) nanoparticles (DNR-GA-Cys-CdTe NPs) was developed. The physical property, drug-loading efficiency and drug release behavior of these DNR-GA-Cys-CdTe NPs were evaluated, and their cytotoxicity was explored by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide assay. These DNR-GA-Cys-CdTe NPs possessed a pH-responsive behavior, and displayed a dose-dependent antiproliferative activity on multidrug-resistant lymphoma Raji/DNR cells. The accumulation of DNR inside the cells, revealed by flow cytometry assay, and the down-regulated expression of P-glycoprotein inside the Raji/DNR cells measured by Western blotting assay indicated that these DNR-GA-Cys-CdTe NPs could minimize the MDR of Raji/DNR cells. This multidrug delivery system would be a promising strategy for minimizing MDR against the lymphoma.

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confidence: 99%

Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo

Zhou¹,

Wang²,

Chen³

et al. 2016

IJN

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“…[24] TCMs have been rarely investigated, and a few TCMs have satisfied chemotherapeutic requirements with effective antitumor efficacy and negligible toxicity. [242526] Wogonin, a representative TCM with negligible toxicity to normal cells,[12] inhibits the proliferation of human cancer cells; however, the underlying molecular mechanisms remain unclear. Studies have mostly identified the association of wogonin with cell cycle arrest and apoptosis,[10] inhibition of tumor angiogenesis,[27] inhibition of tumor cell metastasis by targeting inflammatory microenvironments,[3] and antitumor immunity effect.…”

Section: Discussionmentioning

confidence: 99%

“…MNPs-Fe 3 O 4 have been successfully loaded with doxorubicin,[1531] wogonin,[1032] and gambogic acid. [1425] All demonstrated that MNPs-Fe 3 O 4 could increase the intracellular drug accumulation for cancer cells, thus improving the anticancer effects of the drug. In addition, MNPs-Fe 3 O 4 can be applicable for cancer thermotherapy.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic Mechanism of Human Leukemia K562/A02 Cells Induced by Magnetic Ferroferric Oxide Nanoparticles Loaded with Wogonin

Peng

Wang

et al. 2016

Chinese Medical Journal

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Background:Traditional Chinese medicine wogonin plays an important role in the treatment of leukemia. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. Drugs coated with MNPs are becoming a promising way for better leukemia treatment. This study aimed to assess the possible molecular mechanisms of wogonin-coated MNP-Fe3O4 (Wog-MNPs-Fe3O4) as an antileukemia agent.Methods:After incubated for 48 h, the antiproliferative effects of MNPs, wogonin, or Wog-MNPs-Fe3O4 on K562/A02 cells were determined by methyl thiazolyl tetrazolium (MTT) assay. The apoptotic rates of K562/A02 cells treated with either wogonin or Wog-MNPs-Fe3O4 were determined by flow cytometer (FCM) assay. The cell cycle arrest in K562/A02 cells was determined by FCM assay. The elementary molecular mechanisms of these phenomena were explored by Western blot and reverse transcriptase polymerase chain reaction (RT-PCR).Results:With cell viabilities ranging from 98.76% to 101.43%, MNP-Fe3O4 was nontoxic to the cell line. Meanwhile, the wogonin and Wog-MNPs-Fe3O4 had little effects on normal human embryonic lung fibroblast cells. The cell viabilities of the Wog-MNPs-Fe3O4 group (28.64–68.36%) were significantly lower than those of the wogonin group (35.53–97.28%) in a dose-dependent manner in 48 h (P < 0.001). The apoptotic rate of K562/A02 cells was significantly improved in 50 μmol/L Wog-MNPs-Fe3O4 group (34.28%) compared with that in 50 μmol/L wogonin group (23.46%; P < 0.001). Compared with those of the 25 and 50 μmol/L wogonin groups, the ratios of G0/G1-phase K562/A02 cells were significantly higher in the 25 and 50 μmol/L Wog-MNPs-Fe3O4 groups (all P < 0.001). The mRNA and protein expression levels of the p21 and p27 in the K562/A02 cells were also significantly higher in the Wog-MNPs-Fe3O4 group compared with those of the wogonin group (all P < 0.001).Conclusions:This study demonstrated that MNPs were the effective drug delivery vehicles to deliver wogonin to the leukemia cells. Through increasing cells arrested at G0/G1-phase and inducing apoptosis of K562/A02 cells, MNPs could enhance the therapeutic effects of wogonin on leukemia cells. These findings indicated that MNPs loaded with wogonin could provide a promising way for better leukemia treatment.

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Section: Cellular Delivery Of Qdsmentioning

confidence: 99%

Continuing progress toward controlled intracellular delivery of semiconductor quantum dots

Breger

Delehanty

Medintz

2014

WIREs Nanomed Nanobiotechnol

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The biological applications of luminescent semiconductor quantum dots (QDs) continue to grow at a nearly unabated pace. This growth is driven, in part, by their unique photophysical and physicochemical properties which have allowed them to be used in many different roles in cellular biology including: as superior fluorophores for a wide variety of cellular labeling applications; as active platforms for assembly of nanoscale sensors; and, more recently, as a powerful tool to understand the mechanisms of nanoparticle mediated drug delivery. Given that controlled cellular delivery is at the intersection of all these applications, the latest progress in delivering QDs to cells is examined here. A brief discussion of relevant considerations including the importance of materials preparation and bioconjugation along with the continuing issue of endosomal sequestration is initially provided for context. Methods for the cellular delivery of QDs are then highlighted including those based on passive exposure, facilitated strategies that utilize peptides or polymers and fully active modalities such as electroporation and other mechanically based methods. Following on this, the exciting advent of QD cellular delivery using multiple or combined mechanisms is then previewed. Several recent methods reporting endosomal escape of QD materials in cells are also examined in detail with a focus on the mechanisms by which access to the cytosol is achieved. The ongoing debate over QD cytotoxicity is also discussed along with a perspective on how this field will continue to evolve in the future.

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Synergetic effect of functional cadmium–tellurium quantum dots conjugated with gambogic acid for HepG2 cell-labeling and proliferation inhibition

Cited by 30 publications

References 27 publications

Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo

Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo

Apoptotic Mechanism of Human Leukemia K562/A02 Cells Induced by Magnetic Ferroferric Oxide Nanoparticles Loaded with Wogonin

Continuing progress toward controlled intracellular delivery of semiconductor quantum dots

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