Synergistic Antibacterial Activity of Capsella bursa-pastoris and Glycyrrhiza glabra Against Oral Pathogens

Abstract

Background: Oral infections and dental caries are still considered as serious public health problem and inflict a costly burden to health care services around the world especially in developing countries. Objectives: In the present study, we evaluated the antibacterial activity of Capsella bursa-pastoris alone and also combined with Glycyrrhiza glabra against Streptococcus mutans, S. sanguis, Actinomyces viscosus, Enterococcus faecalis as oral pathogens. Materials and Methods:The antimicrobial activities of an ethanol extract of C. bursa-pastoris alone and in combination with G. glabra were in vitro tested against six reference strains of oral pathogenic bacteria. The antimicrobial activities of the extracts were examined using disc diffusion method and the minimum inhibitory concentration (MIC) determined by both broth and Agar dilution methods and minimum bactericidal concentration (MBC) by broth dilution methods. Results: In this study, C. bursa-pastoris extract showed good antibacterial activity against six bacteria in using in of the mentioned methods. No strain in this study showed resistance against this extract. Antibacterial activity of mixed extract including C .bursa-pastoris and G. glabra was evaluated and showed that mixed extract was more effective against all bacteria than any of the cases alone that indicate the synergistic effect between these two extracts. Conclusions: C. bursa-pastoris and its mixture with G. glabra are suggested as appropriate candidates to control dental caries and endodontic infections.

Shepherd’s Purse Polyphenols Exert Its Anti-Inflammatory and Antioxidative Effects Associated with Suppressing MAPK and NF-κB Pathways and Heme Oxygenase-1 Activation

“…It can not only supply the nutrients but also provide many phytochemicals which play as a functional factor for human health, especially for these people with chronic disease or in the state of subhealth, such as chronic low-grade inflammation; these functional foods may be the best choice to improve their healthy rather than treatment with drugs [39, 40]. Previous studies have proved that shepherd's purse ( Capsella bursa-pastoris ) exerts multihealthy benefits, such as antimicrobial [41], anti-inflammatory [13], cardiovascular, reproductive, anticancer [16, 42], hepatoprotective, sedative, and other pharmacological effects [43]. Therefore, the chemical components of shepherd's purse ethanol extract were first characterized by UPLC-QTOF-MS/MS, and then the anti-inflammatory effects and its underlying mechanisms of SPE in LPS-induced RAW 264.7 inflammation model and in vivo mouse model were also systemically investigated in this study.…”

Antimicrobial Potential of Wild Edible Herbaceous Species

“…Ethanolic extracts were also found active against Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, Enterococcus faecalis, Staphylococcus aureus, E. coli (Soleimanpour et al 2013), and against Pseudomonas aeruginosa and Klebsiella pneumoniae (Hasan et al 2013). …”

Antibacterial effects of Pluchea indica Less leaf extract on E. faecalis and Fusobacterium nucleatum (in vitro)

“…Ester aromatic ring from the gallo and pirogallo groups will bind to protein transport cell envelope of the bacteria, which then will caused protein leakage so that causing damage to cell wall of bacteria and caused bacteria dead. [19][20][21] In addition, nonspecific bonding also occurs through hydrogen bonding of the groups, which can caused damage to the cytoplasmic membrane of the bacteria, so the membrane functions as a selective permeability barrier, carrier active transport function, as well as control of the internal composition of the cell, will be disrupted. Therefore, if the function of the cytoplasmic membrane integrity is damaged, macromolecules and ions will be out of the cell, and then the cell will be damaged and dead.…”