Synergistic Antibacterial Potential of 6-Pentyl-α-pyrone Lactone and Zinc Oxide Nanoparticles against Multidrug-Resistant Enterobacterales Isolated from Urinary Tract Infections in Humans

Abstract

Urinary tract infection (UTI) is one of the most common bacterial infections in the world, which is associated with high morbidity and mortality rates. Enterobacterales species are considered the most causative agent for UTI, especially uropathogenic Escherichia coli (UPEC). Here, we investigated the antibacterial activity of the green fungal metabolite, 6-pentyl α pyrone lactone, alone or in combination with zinc oxide nanoparticles (ZnONPs) against multidrug-resistant Enterobacterales recovered from UTI. The results revealed that 57.27% of human urine samples were positive for Enterobacterales, where E. coli was the most prevalent bacterial pathogen (66.67%). Of note, 98.41% of Enterobacterales isolates were multidrug-resistant (MDR) with multiple antimicrobial resistance (MAR) indices ranged from 0.437 to 1. Fifty percent of the examined isolates were positive for the integrase gene; 60% out of them harbored class 2 integron, whereas the other 40% carried class 1 integrons. The broth microdilution assay ensured that the 6-pentyl-α-pyrone lactone had a reasonable antimicrobial effect against the examined isolates (Minimum inhibitory concentration (MIC) values of 16–32 μg/mL). However, ZnONPs showed a strong antimicrobial effect against the investigated isolates with MIC values ranging from 0.015 to 32 μg/mL. Interestingly, the MICs decreased 5–12 fold and 3–11 fold for 6-pentyl-α-pyrone lactone and ZnONPs, respectively, against examined isolates after their combination. This is the first report suggesting the use of 6-pentyl α pyrone lactone and ZnONPs combination as a promising candidate against MDR Enterobacterales recovered from UTI.

Antibacterial activity of bioactive compounds extracted from red kidney bean (Phaseolus vulgaris L.) seeds against multidrug-resistant Enterobacterales

“…However, a moderate sensitivity level was observed for chloramphenicol (47.37%), followed by ciprofloxacin and tigecycline (40.35 and 42.11%, respectively). In previous reports, high resistance levels were detected for the abovementioned antimicrobial agents (Abd El-Aziz and Gharib, 2015;Elmonir et al, 2021;Kotb et al, 2022).…”

Innovative Insights into In Vitro Activity of Colloidal Platinum Nanoparticles against ESBL-Producing Strains of Escherichia coli and Klebsiella pneumoniae

“…Potent antimicrobial effects, alongside other favorable properties, have been reported for metallic nanoparticles [4,12,56,57]. Although there are many reports regarding the investigation of the antibacterial properties of metallic nanoparticles and application of them, only a minority have included resistant bacterial species [7,58].…”

Antimicrobial Activity of Lactones

“…The antimicrobial effect of ZnONPs against the investigated isolates is shown in the MIC values ranging from 0.015 to 32 µg/mL. Nevertheless, the MICs decreased 5–12-fold for 6-pentyl-α-pyrone lactone ( 40 ) and 3–11-fold for ZnONPs, after their combination [ 62 ].…”