Synergistic Antibacterial Potential of Greenly Synthesized Silver Nanoparticles with Fosfomycin Against Some Nosocomial Bacterial Pathogens

Abstract

Introduction A considerable number of morbidities and fatalities occur worldwide as a result of the multidrug resistant microorganisms that cause a high prevalence of nosocomial bacterial infections. Hence, the current investigation was conducted to evaluate the antibacterial potency of green fabricated silver nanoparticles (AgNPs) against four different nosocomial pathogens. Methods The flower extract of Hibiscus sabdariffa mediated green fabrication of AgNPs and their physicochemical features were scrutinized using different techniques. Antimicrobial activity of the biogenic AgNPs and their synergistic patterns with fosfomycin antibiotic were evaluated using disk diffusion assay. Results and Discussion UV spectral analysis affirmed the successful formation of AgNPs through the detection of broad absorption band at 395 and 524 nm, indicating the surface plasmon resonance of the biofabricated AgNPs. In this setting, the biofabricated AgNPs demonstrated average particle size of 58.682 nm according to transmission electron microscope (TEM) micrographs. The detected hydrodynamic diameter was higher than that noticed by TEM analysis, recording 72.30 nm in diameter and this could be attributed to the action of capping agents, which was confirmed by Fourier Transform Infrared (FT-IR) analysis. Disk diffusion assay indicated the antibacterial potency of biogenic AgNPs (50 μg/disk) against Enterobacter cloacae , Methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli strains with relative inhibition zone diameters of 12.82 ± 0.36 mm, 14.54 ± 0.15 mm, 18.35 ± 0.24 mm and 21.69 ± 0.12 mm, respectively. In addition, E. coli was found to be the most susceptible strain to the biogenic AgNPs. However, the highest synergistic pattern of AgNPs-fosfomycin combination was detected against K. pneumonia strain recording relative synergistic percentage of 64.22%. In conclusion, the detected synergistic efficiency of AgNPs and the antibiotic fosfomycin highlight the potential for utilizing this combination in the biofabrication of effective antibacterial agents against nosocomial pathogens.

Chemical Composition, Antioxidant, Anticancer, and Antibacterial Activities of Roots and Seeds of Ammi visnaga L. Methanol Extract

“…It is noteworthy that Chloramphenicol (20 μg/mL) and 0.1% DMSO served as the positive and negative controls, respectively. MBC determinations were confirmed by spreading the culture from wells that showed no growth onto nutrient agar plates, following the methodology described by [ 90 ].…”

Synergistic Antibacterial Proficiency of Green Bioformulated Zinc Oxide Nanoparticles with Potential Fosfomycin Synergism against Nosocomial Bacterial Pathogens

“…The following bacterial pathogens, comprising Salmonella typhimurium (ATCC 14023), Klebsiella pneumoniae (ATCC 700603), Enterobacter cloacae (ATCC 13047), methicillin-resistant Staphylococcus aureus (ATCC 43300), Escherichia coli (ATCC 25922), and Acinetobacter baumannii (ATCC 43498), were used to determine their sensitivity to the bioprepared ZnO-NPs. Disc diffusion assay was employed to detect the antimicrobial efficacy of the bioinspired ZnO-NPs against the concerned pathogens [ 40 ]. Firstly, the microbial suspension was prepared from fresh bacterial colonies using 0.85% sterilized saline solution then the turbidity was accustomed using 0.5 McFarland standard to acquire viable cell count of 1.0 × 10 8 CFU/mL.…”

Biofabrication of nanoparticles: sources, synthesis, and biomedical applications

“…For biomedical applications in particular, it is essential to characterize them thoroughly to determine whether they are suitable for the intended use. This is accomplished by utilizing several techniques and equipment that can provide the required information ( Aljeldah et al, 2023 ).…”

“…Monodisperse samples have PDI values below 0.05, while samples with a wide range of particle sizes are more likely to have PDI values above 0.7. The high negative charge of the biogenic NPs indicated that the surface of these nanoparticles was negatively charged ( Aljeldah et al, 2023 ). Using Brookhaven Zeta Plus and dynamic light scattering (DLS) measurements, the hydrodynamic size of the phytofabricated NPs and PDI were assessed using nanoparticle-tracking analysis.…”