Synergistic approach for treatment of chicken coccidiosis using berberine – A plant natural product

Malik, Tauseef Ahmad; Kamili, Azra N.; Chishti, M. Z.; Tanveer, Syed; Ahad, Shazia; Johri, R.K.

doi:10.1016/j.micpath.2016.01.012

Cited by 21 publications

(11 citation statements)

References 51 publications

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“…Berberine effectively controls coccidial infection and Clostridium perfringens-associated necrotic enteritis in broilers [18,19,20]. In addition, berberine is a good alternative feed additive and has been used safely in starter, grower, and finisher feeds for broilers, as the commercial poultry industry has faced increasing pressure to reduce the use of antimicrobial growth promoters.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Berberine on Broiler P-glycoprotein Expression and Function: In Situ and In Vitro Studies

Zhang

Guo

Huang

et al. 2019

IJMS

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Overcoming P-glycoprotein (P-gp) efflux is a strategy to improve the absorption and pharmacokinetics of its substrate drugs. Berberine inhibits P-gp and thereby increases the bioavailability of the P-gp substrate digoxin in rodents. However, the effects of berberine on P-gp in chickens are still unclear. Here, we studied the role of berberine in modulating broilers P-gp expression and function through both in situ and in vitro models. In addition, molecular docking was applied to analyze the interactions of berberine with P-gp as well as with chicken xenobiotic receptor (CXR). The results showed that the mRNA expression levels of chicken P-gp and CXR decreased in the ileum following exposure to berberine. The absorption rate constant of rhodamine 123 increased after berberine treatment, as detected using an in situ single-pass intestinal perfusion model. Efflux ratios of P-gp substrates (tilmicosin, ciprofloxacin, clindamycin, ampicillin, and enrofloxacin) decreased and the apparent permeability coefficients increased after co-incubation with berberine in MDCK-chAbcb1 cell models. Bidirectional assay results showed that berberine could be transported by chicken P-gp with a transport ratio of 4.20, and this was attenuated by verapamil (an inhibitor of P-gp), which resulted in a ratio of 1.13. Molecular docking revealed that berberine could form favorable interactions with the binding pockets of both CXR and P-gp, with docking scores of −7.8 and −9.5 kcal/mol, respectively. These results indicate that berberine is a substrate of chicken P-gp and down-regulates P-gp expression in chicken tissues, thereby increasing the absorption of P-gp substrates. Our findings suggest that berberine increases the bioavailability of other drugs and that drug-drug interactions should be considered when it is co-administered with other P-gp substrates with narrow therapeutic windows.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Berberine on Broiler P-glycoprotein Expression and Function: In Situ and In Vitro Studies

Zhang

Guo

Huang

et al. 2019

IJMS

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“…Natural products, such as cider vinegar, are also emerging as alternative strategies to control avian coccidiosis. [65][66][67][68] Fungal Nephritis…”

Section: Parasitic Nephritismentioning

confidence: 99%

Clinical Management of Avian Renal Disease

Cojean

Larrat

Vergneau‐Grosset

2020

Veterinary Clinics of North America: Exotic Animal Practice

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“…The various Eimeria species infect different areas of the chicken intestinal tract [ 5 , 6 ], thus Eimeria species may exhibit differences in susceptibility to alternative products, such as herbal products, essential oils, organic minerals, and probiotics [ 25 ]. One of the alternative products is berberine that has potential anticoccidial properties as demonstrated within a limited range, namely in E. tenella infected-chickens [ 14 , 24 ]. Therefore, this study investigated whether berberine differentially affects chickens infected with various species of Eimeria , E. acervulina , E. maxima , E. tenella , E. mitis , and E. praecox .…”

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confidence: 99%

“…2 ), indicating that berberine had anticoccidial activity only in vivo. Interestingly, previous studies of E. tenella showed that berberine-treated, E. tenella -infected chickens significantly reduced fecal oocyst shedding [ 14 , 24 ]. It is speculated that anticoccidial activity of berberine may be partly mediated by generation of a redox imbalance and depolarization of mitochondrial membrane [ 28 ], by impairment of intracellular development and multiplication of Eimeria [ 12 ] or by inhibition of telomerase activity [ 21 ].…”

mentioning

confidence: 99%

“…Several natural products have been examined for potential therapeutic and prophylactic effects against Eimeria, one of which is berberine [9,[11][12][13][14]. Berberine is a yellow isoquinoline alkaloid extracted from the stems and roots of various plants, such as Berberis, Hydrastis canadensis, and Coptidis rhizoma, and is used in Chinese medicine to treat gastrointestinal diseases [15,16].…”

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Anticoccidial Activity of Berberine against Eimeria-Infected Chickens

et al. 2021

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Avian coccidiosis has a major economic impact on the poultry industry, it is caused by 7 species of Eimeria, and has been primarily controlled using chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. We assessed anticoccidial effects of berberine-based diets in broiler chickens following oral infection with 5 Eimeria species (E. acervulina, E. maxima, E. tenella, E. mitis, and E. praecox). When 0.2% berberine, a concentration that does not affect weight gain, was added to the diet, the 4 groups infected with E. acervulina, E. tenella, E. mitis, or E. praecox showed significant reductions in fecal oocyst shedding (P<0.05) compared to their respective infected and untreated controls. In chickens treated 0.5% berberine instead of 0.2% and infected with E. maxima, fecal oocyst production was significantly reduced, but body weight deceased, indicating that berberine treatment was not useful for E. maxima infection. Taken together, these results illustrate the applicability of berberine for prophylactic use to control most Eimeria infections except E. maxima. Further studies on the mechanisms underlying the differences in anticoccidial susceptibility to berberine, particularly E. maxima, are remained.

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Synergistic approach for treatment of chicken coccidiosis using berberine – A plant natural product

Cited by 21 publications

References 51 publications

Inhibitory Effect of Berberine on Broiler P-glycoprotein Expression and Function: In Situ and In Vitro Studies

Inhibitory Effect of Berberine on Broiler P-glycoprotein Expression and Function: In Situ and In Vitro Studies

Clinical Management of Avian Renal Disease

Anticoccidial Activity of Berberine against Eimeria-Infected Chickens

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