Synergistic combination of cryoprotectants for high freeze-dried survival rate and viable cell counts of Streptococcus thermophilus

Combinedly increased viability of Lactiplantibacillus plantarum grx16 by co‐encapsulation of cryoprotectants and porous starch within calcium alginate capsules

“…Due to casein nanoparticles contained in skim milk of the cryoprotectants could cover the probiotic surface, stabilising the cell membrane/wall constituents, and form porous structure after freeze‐drying, the surface of freeze‐dried CAC‐Pro capsules became coarse and loose (Fig. 1B2, B3), which would be conducive to the rehydration of these capsules (Lu et al ., 2017; Di et al ., 2022).…”

Synergistic combination of cryoprotectants improved freeze‐dried survival rate and viable counts of Lactiplantibacillus plantarum

“…The freeze‐drying method used was as described previously (Di et al . 2022). The ampoule bottles with bacteria were pre‐freezing (−60°C) for 12 h. Simultaneously, viable cell count before freeze‐drying was determined.…”

“…Single cryoprotectant usually cannot achieve good protective effects. Therefore, it is necessary to synergistically combine multiple types of cryoprotectants to enhance the protective effect (Chin et al 2021;Ta et al 2021;Wang et al 2021;Di et al 2022). However, the combination principle of cryoprotectants is not yet clear.…”