Synergistic effects of cAMP–dependent protein kinase A and AMP-activated protein kinase on lipolysis in kinsenoside-treated C3H10T1/2 adipocytes

Lee, Yuan Chii G.; Sue, Yuh Mou; Lee, Ching Kuo; Huang, Huei Mei; He, Jhin Jyun; Wang, Yu Shiou; Juan, Shu Hui

doi:10.1016/j.phymed.2018.06.043

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…Furthermore, inhibiting the cAMP/PKA signaling pathway reversed the effects of DHA on stimulating lipolysis and reducing lipid accumulation, implying that the cAMP/PKA signaling pathway may participate in DHA-induced adipocyte lipolysis in grass carp. These findings are consistent with the study on kinsenoside-treated adipocytes, that H89 eliminated PKA-dependent lipid reduction by inhibiting the phosphorylation of HSL and perilipin activation ( Lee et al., 2019 ). Further analyses revealed that 4-PBA treatment inhibited DHA-induced ER stress and DHA-induced activation of the cAMP/PKA signaling pathway, suggesting that the activation of ER stress promoted lipolysis might be via the cAMP/PKA signaling pathway.…”

Section: Discussionsupporting

confidence: 92%

DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella)

Huang¹,

Bian²,

Ji³

et al. 2023

Animal Nutrition

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Section: Discussionsupporting

confidence: 92%

DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella)

Huang¹,

Bian²,

Ji³

et al. 2023

Animal Nutrition

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“…The relative abundance of the target protein was measured using the ImageJ software and normalized to the intensity of the β-actin band. The subsequent procedures are described in a previous study 23 .…”

Section: Methodsmentioning

confidence: 99%

l-Carnitine reduces reactive oxygen species/endoplasmic reticulum stress and maintains mitochondrial function during autophagy-mediated cell apoptosis in perfluorooctanesulfonate-treated renal tubular cells

Lee

Chou

Chen

et al. 2022

Sci Rep

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We previously reported that perfluorooctanesulfonate (PFOS) causes autophagy-induced apoptosis in renal tubular cells (RTCs) through a mechanism dependent on reactive oxygen species (ROS)/extracellular signal-regulated kinase. This study extended our findings and determined the therapeutic potency of l-Carnitine in PFOS-treated RTCs. l-Carnitine (10 mM) reversed the effects of PFOS (100 µM) on autophagy induction and impaired autophagy flux. Furthermore, it downregulated the protein level of p47Phox, which is partly related to PFOS-induced increased cytosolic ROS in RTCs. Moreover, l-Carnitine reduced ROS production in mitochondria and restored PFOS-impeded mitochondrial function, leading to sustained normal adenosine triphosphate synthesis and oxygen consumption and reduced proton leakage in a Seahorse XF stress test. The increased inositol-requiring enzyme 1α expression by PFOS, which indicated endoplasmic reticulum (ER) stress activation, was associated with PFOS-mediated autophagy activation that could be attenuated through 4-phenylbutyrate (5 mM, an ER stress inhibitor) and l-Carnitine pretreatment. Therefore, by reducing the level of IRE1α, l-Carnitine reduced the levels of Beclin and LC3BII, consequently reducing the level of apoptotic biomarkers including Bax and cleaving PARP and caspase 3. Collectively, these results indicate that through the elimination of oxidative stress, extracellular signal–regulated kinase activation, and ER stress, l-Carnitine reduced cell autophagy/apoptosis and concomitantly increased cell viability in RTCs. This study clarified the potential mechanism of PFOS-mediated RTC apoptosis and provided a new strategy for using l-Carnitine to prevent and treat PFOS-induced RTC apoptosis.

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“…Hepatocytes and adipocytes, the principal regulators controlling lipid droplets anabolism and catabolism, are verified to be the main sites targeted by medications aimed to manage dyslipidemia. A number of studies have supported the versatile lipid-lowering abilities of KD, as exemplified by decreasing blood total cholesterol and triglyceride (TG) content, reducing hepatocellular TG level and enhancing activity of adipocyte-related lipolysis ( Du et al, 2001 ; Liu et al, 2013 ; Cheng et al, 2015 ; Lee et al, 2019 ). Cumulative evidence indicates that pro-inflammatory stimuli had the capacity of inducing fatty acid and cholesterol biosynthesis, TG lipolysis, and very low-density lipoprotein and free fatty acid secretion while suppressing fatty acid oxidation and cholesterol excretion in cell types responsible for lipid metabolism ( Khovidhunkit et al, 2004 ; Glass and Olefsky, 2012 ).…”

Section: Potentials Of Kd Against Disease Developmentmentioning

confidence: 99%

Advances in the therapeutic application and pharmacological properties of kinsenoside against inflammation and oxidative stress-induced disorders

et al. 2022

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Extensive research has implicated inflammation and oxidative stress in the development of multiple diseases, such as diabetes, hepatitis, and arthritis. Kinsenoside (KD), a bioactive glycoside component extracted from the medicinal plant Anoectochilus roxburghii, has been shown to exhibit potent anti-inflammatory and anti-oxidative abilities. In this review, we summarize multiple effects of KD, including hepatoprotection, pro-osteogenesis, anti-hyperglycemia, vascular protection, immune regulation, vision protection, and infection inhibition, which are partly responsible for suppressing inflammation signaling and oxidative stress. The protective action of KD against dysfunctional lipid metabolism is also associated with limiting inflammatory signals, due to the crosstalk between inflammation and lipid metabolism. Ferroptosis, a process involved in both inflammation and oxidative damage, is potentially regulated by KD. In addition, we discuss the physicochemical properties and pharmacokinetic profiles of KD. Advances in cultivation and artificial synthesis techniques are promising evidence that the shortage in raw materials required for KD production can be overcome. In addition, novel drug delivery systems can improve the in vivo rapid clearance and poor bioavailability of KD. In this integrated review, we aim to offer novel insights into the molecular mechanisms underlying the therapeutic role of KD and lay solid foundations for the utilization of KD in clinical practice.

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Synergistic effects of cAMP–dependent protein kinase A and AMP-activated protein kinase on lipolysis in kinsenoside-treated C3H10T1/2 adipocytes

Cited by 10 publications

References 31 publications

DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella)

DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella)

l-Carnitine reduces reactive oxygen species/endoplasmic reticulum stress and maintains mitochondrial function during autophagy-mediated cell apoptosis in perfluorooctanesulfonate-treated renal tubular cells

Advances in the therapeutic application and pharmacological properties of kinsenoside against inflammation and oxidative stress-induced disorders

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