Synergistic inhibition of sunitinib and ethaselen against human colorectal cancer cells proliferation

Zheng, Xiaoqing; Zhang, Yunhan; Zhang, Lei; Xu, Wei; Ma, Weiwei; Sun, Ruoxuan

doi:10.1016/j.biopha.2016.06.040

Cited by 30 publications

(16 citation statements)

References 35 publications

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“…CRC is the third most common and one of the most frequent cause of cancer-related death worldwide [ 1 ]. Increased cell survival capability is critical during CRC tumorigenesis and progression [ 21 ]. Previously, people mainly focused their attentions on proteins with oncogenic or tumor-suppressing roles, such as KRAS, p53, and adenomatous polyposis coli (APC) [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA HEIH Promotes Colorectal Cancer Tumorigenesis via Counteracting miR-939‒Mediated Transcriptional Repression of Bcl-xL

et al. 2018

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PurposeStudies have found that long noncoding RNA HEIH (lncRNA-HEIH) is upregulated and facilitates hepatocellular carcinoma tumor growth. However, its clinical significances, roles, and action mechanism in colorectal cancer (CRC) remains unidentified.Materials and MethodslncRNA-HEIH expression in CRC tissues and cell lines was measured by quantitative real-time polymerase chain reaction. Cell CountingKit-8, ethynyl deoxyuridine incorporation assay, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and nude mice xenografts assays were performed to investigate the roles of lncRNA-HEIH. RNA pull-down, RNA immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays were performed to investigate the action mechanisms of lncRNA-HEIH.ResultsIn this study, we found that lncRNA-HEIH is significantly increased in CRC tissues and cell lines. lncRNA-HEIH expression is positively associated with tumor size, invasion depth, and poor prognosis of CRC patients. Enhanced expression of lncRNA-HEIH promotes CRC cell proliferation and decreases apoptosis in vitro, and promotes CRC tumor growth in vivo. Whereas knockdown of lncRNA-HEIH inhibits CRC cell proliferation and induces apoptosis in vitro, and suppresses CRC tumor growth in vivo. Mechanistically, lncRNA-HEIH physically binds to miR-939. The interaction between lncRNA-HEIH and miR-939 damages the binding between miR-939 and nuclear factor κB (NF-κB), increases the binding of NF-κB to Bcl-xL promoter, and promotes the transcription and expression of Bcl-xL. Moreover, Bcl-xL expression is positively associatedwith lncRNA-HEIH in CRC tissues. Blocking the interaction between lncRNA-HEIH and miR-939 abolishes the effects of lncRNA-HEIH on CRC tumorigenesis.ConclusionThis study demonstrated that lncRNA-HEIH promotes CRC tumorigenesis through counteracting miR-939‒mediated transcriptional repression of Bcl-xL, and suggested that lncRNA-HEIH may serve as a prognostic biomarker and therapeutic target for CRC.

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Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA HEIH Promotes Colorectal Cancer Tumorigenesis via Counteracting miR-939‒Mediated Transcriptional Repression of Bcl-xL

et al. 2018

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“…Combination of cisplatin and ethaselen produced similar results in a leukemic cisplatin resistant cell line with substantial synergistic effects. Ethaselen with Sunitinib (multitargeted tyrosine kinase inhibitor) combination has exhibited similar synergistic effects against spreading of colorectal cancer cells (Zheng et al 2016). In spite of ethaselen encouraging anticancer activity, its solubility in physiological media is not ideal, thus preventing its application without appropriate formulation.…”

Section: Ethaselenmentioning

confidence: 99%

Selenium and selenoproteins: it’s role in regulation of inflammation

2020

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Selenium is an essential immunonutrient which holds the human's metabolic activity with its chemical bonds. The organic forms of selenium naturally present in human body are selenocysteine and selenoproteins. These forms have a unique way of synthesis and translational coding. Selenoproteins act as antioxidant warriors for thyroid regulation, male-fertility enhancement, and anti-inflammatory actions. They also participate indirectly in the mechanism of wound healing as oxidative stress reducers. Glutathione peroxidase (GPX) is the major selenoprotein present in the human body, which assists in the control of excessive production of free radical at the site of inflammation. Other than GPX, other selenoproteins include selenoprotein-S that regulates the inflammatory cytokines and selenoprotein-P that serves as an inducer of homeostasis. Previously, reports were mainly focused on the cellular and molecular mechanism of wound healing with reference to various animal models and cell lines. In this review, the role of selenium and its possible routes in translational decoding of selenocysteine, synthesis of selenoproteins, systemic action of selenoproteins and their indirect assimilation in the process of wound healing are explained in detail. Some of the selenium containing compounds which can acts as cancer preventive and therapeutics are also discussed. These compounds directly or indirectly exhibit antioxidant properties which can sustain the intracellular redox status and these activities protect the healthy cells from reactive oxygen species induced oxidative damage. Although the review covers the importance of selenium/selenoproteins in wound healing process, still some unresolved mystery persists which may be resolved in near future.

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“…It has also been used for the treatment of some other malignancies, including colon cancer, breast cancer, neuro-endocrine cancer, and lung cancer in preclinical and clinical presentations [5][6][7]. It is a novel, multi-targeted receptor and tyrosine kinase inhibitor that impedes signaling through platelet-derived growth factor receptors (PDGFRs), vascular endothelial growth factor receptors (VEGFRs), and stem cell factor receptor (KIT) [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Characteristics and anticancer properties of Sunitinib malate-loaded poly-lactic-co-glycolic acid nanoparticles against human colon cancer HT-29 cells lines

Alshetaili¹,

Anwer²,

Alshahrani³

et al. 2018

Trop. J. Pharm Res

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Purpose: To develop poly-lactic-co-glycolic acid (PLGA)-based nanoparticles (NPs) for the delivery of sunitinib malate (STM) to colon cancer cells. Methods: Three different formulations (F1-F3) were developed by nano-precipitation technique using various concentrations of PLGA. The NPs were evaluated for particle size, polydispersity index, zeta potential, drug entrapment, and drug loading, using differential scanning calorimetry (DSC), Fouriertransform infrared spectroscopy (FTIR), x-ray diffraction (XRD), and scanning electron microscopy (SEM). Furthermore, in vitro drug release and anticancer studies were carried out on the formulations. Results: Among the three NPs, optimized NP (F3) of STM was chosen for in vitro anti-cancer study against H-29 human colon cancer cells lines based on its particle size (132.9 nm), PDI (0.115), zeta potential (-38.12 mV), entrapment efficiency (52.42 %), drug loading (5.24 %), and drug release (91.26 % in 48 h). A significant anti-cancer activity of the optimized NPs was observed, relative to free STM. Conclusion: These findings suggest that STM-loaded NPs possess significant anti-cancer activity against human colon cancer HT-29 cells lines.

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Synergistic inhibition of sunitinib and ethaselen against human colorectal cancer cells proliferation

Cited by 30 publications

References 35 publications

Long Noncoding RNA HEIH Promotes Colorectal Cancer Tumorigenesis via Counteracting miR-939‒Mediated Transcriptional Repression of Bcl-xL

Long Noncoding RNA HEIH Promotes Colorectal Cancer Tumorigenesis via Counteracting miR-939‒Mediated Transcriptional Repression of Bcl-xL

Selenium and selenoproteins: it’s role in regulation of inflammation

Characteristics and anticancer properties of Sunitinib malate-loaded poly-lactic-co-glycolic acid nanoparticles against human colon cancer HT-29 cells lines

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