Synergistic Protective Effect of Konjac Mannan Oligosaccharides and Bacillus subtilis on Intestinal Epithelial Barrier Dysfunction in Caco-2 Cell Model and Mice Model of Lipopolysaccharide Stimulation

Abstract

As the first line of defense against intestinal bacteria and toxins, intestinal epithelial cells are always exposed to bacteria or lipopolysaccharide (LPS), whereas pathogenic bacteria or LPS can cause intestinal epithelial cell damage. Previous studies have shown that konjac mannan oligosaccharides (KMOS) have a positive effect on maintaining intestinal integrity, and Bacillus subtilis (BS) can promote the barrier effect of the intestine. However, it is still unknown whether KMOS and BS have a synergistic protective effect on the intestines. In this study, we used the LPS-induced Caco-2 cell injury model and mouse intestinal injury model to study the synergistic effects of KMOS and BS. Compared with KMOS or BS alone, co-treatment with KMOS and BS significantly enhanced the activity and antioxidant capacity of Caco-2 cell, protected mouse liver and ileum from LPS-induced oxidative damage, and repaired tight junction and mucus barrier damage by up-regulating the expression of Claudin-1, ZO-1 and MUC-2. Our results demonstrate that the combination of KMOS and BS has a synergistic repair effect on inflammatory and oxidative damage of Caco-2 cells and aIIeviates LPS-induced acute intestinal injury in mice.

Lactobacillus acidophilus LA85 ameliorates cyclophosphamide-induced immunosuppression by modulating Notch and TLR4/NF-κB signal pathways and remodeling the gut microbiota

“…25 LPS is an endotoxin secreted by intestinal microorganisms associated with the permeability of the intestine. 26 d -LA is a bacterial metabolite that can enter the circulation in the body through damaged mucosa. 27 Therefore, serum DAO, LPS, and d -LA levels are widely used to evaluate the permeability of the intestinal mucosa.…”

Association of Gut Microbiota With Intestinal Ischemia/Reperfusion Injury

“…The gut is the core organ of human immunity, in which 60-70% of the immune cells of the human body are located in the gut to resist the invasion of foreign germs and toxins ( Hou et al., 2020 ; Fan et al., 2021 ; Liu et al., 2021 ). In the daily diet and metabolism, the intestinal tract gathers the most important garbage and toxins in the human body, and will eventually be excreted through the feces, which is also an important channel for the excretion of more than 80% of the toxins in the human body ( Zhao et al., 2021 ; Butt and Jenab, 2021 ; Chen et al., 2021 ). In addition, more than 97.5% of the nutrients necessary for the human body are obtained from the intestinal absorption ( Tiffany et al., 2021 ); 95% of the core important hormones of the human body are produced by the intestinal tract, and it is currently known that the human gastrointestinal tract can secrete more than 35 kinds of hormones ( Mayneris-Perxachs et al., 2020 ; Lin et al., 2021 ; Qi et al., 2021 ).…”

In vitro assessment of the probiotic properties of an industrial preparation containing Lacticaseibacillus paracasei in the context of athlete health

“…In the intestinal mucosa, oxidative stress and inflammation are interconnected, and contribute to epithelial barrier damage ( Chen et al, 2021 ). Potentially, the AApf ability of reducing ROS in enterocytes, together with the observed anti-inflammatory activity and epithelial barriers preservation, could contribute to protect the integrity of the intestinal mucosa under the stress conditions generated by the strenuous physical activity.…”