Synovium in the pathophysiology of osteoarthritis

Monemdjou, Roxana; Fahmi, Hassan; Kapoor, Mohit

doi:10.2217/thy.10.72

Cited by 10 publications

(14 citation statements)

References 34 publications

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“…Production of IL-6 in disease-affected joint tissue is a response from IL-1β and TNF-α [8]. IL-1β is mostly produced by synovial macrophages and chondrocytes in the OA joint [9]. The synovial intimal cells, termed synoviocytes, are believed to be responsible for the production of synovial fluid components, for absorption from the joint cavity, and for blood/synovial fluid exchanges.…”

Section: Discussionmentioning

confidence: 99%

“…Interlukin-1β (IL-1β) is considered as one of the most important and most influential cytokines in its involvement in the OA pathogenesis. IL-1β is mostly produced by synovial macrophages and chondrocytes in the OA joint [9]. These cytokines can induce inflammatory reactions and catabolic influences independently [8].…”

Section: Interleukin 1βmentioning

confidence: 99%

“…These cytokines can induce inflammatory reactions and catabolic influences independently [8]. The role of IL-1β in pathogenesis is to suppress type II collagen production and proteoglycan and stimulate synovial cells to release matrix metalloproteinase (MMP) [9]. Cytokine IL-1β also releases proteoglycan from the extracellular matrix towards synovial fluid, in addition IL-1β can inhibit the synthesis of collagen type II, IX, and XI, stimulate abnormal proteoglycan production, and reduce the expression of natural MMPs inhibitors called tissue inhibitors of metalloproteinase (TIMP) [17].…”

Section: Interleukin 1βmentioning

confidence: 99%

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The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV

Rahmadian¹,

Manjas²,

Silvia³

et al. 2019

Proceedings of the Proceedings of the 1st EAI International Conference on Medical and Health Research, ICoMHER November 13-14th

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Osteoarthritis is a degenerative joint disease involving activated synovial tissue due to inflammation. Cytokines seem to enhance the matrix components of lead to destruction of cartilage. Mesenchymal stem cells (MSCs) are widely used for cell-based nowadays approaches, thus activated cytokines, IL-1β andIL-6 as a result of synovial inflammation that are left to be investigated.This is an in vitro study of IV-IL and IL-6 on IV synoviocytes-derived osteoarthritis treated with MSCs. Synovium was harvested from 3 patients with grade IV OA who were diagnosed by orthopedic surgeon and TKR underwent. Cells were isolated using explants culture method and cultured in DMEM with 10% fetal bovine serum, 1% penicillin streptomycin, and amphotericin B. Cells were incubated at 370C and 5% CO2. After third passages, cells were co-cultured with MSCs. Expression of IL-1β and IL-6 are observed using realtime polymerase chain reaction (PCR) after 24 hours and 48 hours. The expression of IL-1β is marked up after 24 hours of cocultured with MSCs (57.72 ± 18.37) compared to control and shows a tendency to downgrade after 48 hours of event, statictically not significant. However, IL-6 showed a higher expression in synoviocytes after 24 hours of co-cultured with MSCs, and was significantly down regulated after 48 hours. Both IL-1 β and IL-6 showed higher expressions on 24 hours co-culturing synoviocytes with MSCs. Not IL-1β, expression of IL-6 is significantly decreased after 48 hours MSC treatment, implying that MSV grade IV syndicarthritis-derived osteoarthritis.

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Section: Discussionmentioning

confidence: 99%

Section: Interleukin 1βmentioning

confidence: 99%

Section: Interleukin 1βmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV

Rahmadian¹,

Manjas²,

Silvia³

et al. 2019

Proceedings of the Proceedings of the 1st EAI International Conference on Medical and Health Research, ICoMHER November 13-14th

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“…TIMP-1 is a protective mediator and appears to be exceptionally high in OA in synovial fluid. TIMP-1 and TIMP-2 are both present in the SM of OA [13], [14], [15]. The expression of mRNA from TIMP by chondrocytes in OA cartilage is higher than TIMP by normal cartilage chondrocytes.…”

Section: Pathogenesismentioning

confidence: 92%

“…These cytokines also increase the expression of inflammatory mediators and the activity of several proteolytic enzymes such as MMPs and aggrecanase that play a role in degrading joint cartilage [10], [13], [20], [21], [22]. Molecules included in pro-inflammatory cytokines include IL-1β (IL-1β), TNFα, IL-6, IL-15, IL-17, and IL-18 [7], [13], [14], [16], [23] ( Figure 1).…”

Section: Pathogenesismentioning

confidence: 99%

Clinical Application Prospect of Human Synovial Tissue Stem Cells from Osteoarthritis Grade IV Patients in Cartilage Regeneration

Rahmadian

Adly

Dilogo

et al. 2021

Open Access Maced J Med Sci

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Osteoarthritis (OA) is a joint problem that continues to increase in prevalence as life expectancy increases. OA can affect any joint, especially those that support body weight such as the knee and hip joint. Although both primary and secondary OA have the same clinical symptoms, it can be caused by different etiologies. OA is no longer considered a degenerative disease, although age is still a major factor. Various attempts have been made to regenerate joint cartilage damaged by OA. The use of stem cells in OA therapy is a very promising opportunity. Stem cells are undifferentiated biological cells and are multipotent to differentiate into specific cells. In principle, local stem cells are the best source of stem cells to regenerate the surrounding tissue. The synovial membrane is a tissue in the joint that can regenerate. After synovectomy surgery, repair, and growth of synovial tissue occur rapidly. Synovial tissue as a source of stem cells only provides a limited amount. One source of synovial tissue that can be used is tissue taken from the total knee replacement process in grade 4 OA patients. However, it is necessary to prove the potential of synovial tissue stem cells originating from old-age donors.

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Mechanically-induced osteophyte in the rat knee

Venne

Tse

Pang

et al. 2020

Osteoarthritis and Cartilage

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Objectives: Osteophytes are common anatomical signs of advanced osteoarthritis. It remains unclear whether they develop from physio-molecular, and/or mechanical stimuli. This study examined the effects of mechanical impact on the knee joint periosteum leading to osteophyte formation. Design: Eighteen mature rats received one single impact load of 53 N (30 MPa) to the periosteum of the experimental medial femoral condyles. Contralateral knees were used as controls. Animals were sacrificed at 24 h, 3, 6 and 9 weeks post-impact. Distal femurs were harvested and prepared for histology. Hematoxylin and Eosin, and Masson's trichrome stained slides were examined by light microscopy. Nuclear density was quantified to assess the tissue reaction. Results: 24 h: The synovium membrane, fibrous and cambium periosteum were damaged. Blood infiltration pooled in the impacted medial collateral ligament (MCL) region. Week 3: A cartilaginous tissue spur, chondrophyte, was found in every rat at the impacted site of the MCL. Chondrophytes were composed of fibrocartilage and cartilage matrix, with signs of cartilage mineralization and remodelling activity. Week 6: Chondrophytes presented signs of more advanced mineralisation, recognized as osteophytes. Week 9: Osteophytes appeared to be more mineralized with almost no cartilage tissue. Conclusions: Osteophytes can be induced with a single mechanical impact applied to the periosteum in rat knees. These data indicate that a moderate trauma to the periosteal layer of the joint may play a role in osteophyte development.

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Synovium in the pathophysiology of osteoarthritis

Cited by 10 publications

References 34 publications

The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV

The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV

Clinical Application Prospect of Human Synovial Tissue Stem Cells from Osteoarthritis Grade IV Patients in Cartilage Regeneration

Mechanically-induced osteophyte in the rat knee

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