Synthesis and Biocompatibility of Maleic Anhydride Copolymers: 1. Maleic Anhydride-Vinyl Acetate, Maleic Anhydride-Methyl Methacrylate and Maleic Anhydride-Styrene

Abstract

A series of maleic anhydride (MA)–vinyl acetate (VA), MA–methyl methacrylate (MM) and MA–styrene (S) copolymers were prepared and characterized. By employing various amounts of initiator, MA–VA, MA–MM and MA–S copolymers with molecular weights ranging between 18 000 and 219 000 were obtained. The ‘in vivo’ and ‘in vitro’ tests performed on K562 cellular cultures (human chronic myeloid leukaemia) and also on Westar rats (inoculated with the Walker 256 carcinosarcome) showed that, as a function of the molecular weight, the copolymers synthesized had a 50% in vitro cytotoxicity and a mean tumour regression of a maximum of 68%. © 1997 SCI.

Biological Effects and Antitumor Activity Induced by Benzocaine Conjugated Anionic Polymers

“…The biochemical screening included: (a) protein profile [17,18]: serum proteins electrophoresis, total serum protein (Lowry method) and serum creatinine (alkaline picrate or Jaffé reaction); (b) lipoprotein profile [22,23]: lipoprotein electrophoresis, the major serum lipids (phospho-vanillin reaction), serum triglycerides (phenyl hydrazine reaction), total serum cholesterol (colorimetric reaction with acetic anhydride, acetic acid and sulfuric acid), serum esterified cholesterol (precipitated with digitonin), LDL-cholesterol (precipitated with heparin in the presence of the divalent ions) and HDL-cholesterol (precipitated with trichloroacetic acid and measured as cholesterol in the supernatant of serum after precipitation of the other lipoproteins with heparin); (c) glucose and enzyme profile [22,23]: serum glucose (otoluidine method), serum glutamic oxalacetic transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum lactate dehydrogenase (LDH) and serum amylase -according to Randox kit.…”

“…Pyran copolymer was obtained from Hercules, Inc. Delaware and the maleic anhydride copolymers with vinyl acetate (MA-VA), styrene (MA-St) and methyl methacrylate (MA-MM) were synthesized at the Macromolecular Chemistry Institute, Iasi, Romania, according to reported procedures [17][18][19] as well as the maleic anhydride copolymers that were modified with benzocaine. For the biological assays the polymers were dissolved in normal saline solution and administered ip, for 7 days, at a dose of 20 mg/kg/day.…”

“…The unmodified MA-VA copolymers series increased the SGPT and decreased the SGOT and serum amylase with increasing molecular weight; also, the low molecular weight fraction (12,900) increased the lactate dehydrogenase (LDH). In the modified series, the high molecular weight fraction (23,000) increased the SGOT, SGPT and both fractions increased the LDH; only the low molecular weight fraction (18,200) increased the serum amylase. The unmodified MA-St copolymer increased the SGPT level and both series decreased SGOT; the modified copolymer increased the serum LDH.…”

“…This pyran as well as specific molecular weight fractions of MVE (1-6) have been investigated extensively from the standpoint of both its chemical structure and its antitumor activity [7][8][9][10][11]. Based on the structural studies of DIVEMA, a large number of additional copolymers based on maleic anhydride were synthesized and characterized [12][13][14][15][16][17][18]. Many of these copolymers were evaluated for their antitumor properties by the NIH.…”

Synthesis and properties of starch-g-poly(maleic anhydride-co-vinyl acetate)

“…The sample was weighed, kept in NaOH (30 ml, 0.09674 M) at 60°C for 3 h, and titrated with HCl (0.07289 M) in the presence of thymol blue. Then the carboxylic unit percentage (CUP) of SMV could be calculated according to Equation (2) [19]: (2) where C, V and m were the concentration, volume and weight of NaOH, HCl and SMV respectively. The average CUP was taken from four samples.…”