Synthesis and characterization of conductive neural tissue engineering scaffolds based on urethane-polycaprolactone

Nazarpak, Masoumeh Haghbin; Entekhabi, Elahe; Najafi, Farhood; Rahmani, M.R.; Solati‐Hashjin, Mehran

doi:10.1080/00914037.2018.1513933

Cited by 11 publications

(6 citation statements)

References 49 publications

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“…[365] Polydopamine and RGD layers facilitated cell adhesion while PCL/graphene layers enabled local electrical activity. Using graphene as the conductive element, these multilayered conduits achieved around two orders of magnitude higher conductivity (around 6 × 10 −3 S cm −1 ) than similar multilayered conduits based on PPy [316] or PANI. [366] When seeded with Schwann cells prior to implantation, these graphene-based conduits improved peripheral nerve repair.…”

Section: Porous 3d and Tubular Scaffoldsmentioning

confidence: 93%

“…Song et al [ 316] DBS-doped 2D PPy films and 3D PPy tubular scaffolds Human iPSC-derived NPC 40 V m −1 for 1 h Applied stimulation increased the expression of neurotrophic factors from cells seeded in 3D scaffold. However, there was higher cell viability on 2D films.…”

Section: Icr Mouse (1 D Postnatal) Hippocampal Nscsmentioning

confidence: 99%

“…[315] In a recent study, tubular scaffolds of DBS-doped PPy promoted increased expression of neurotrophic factors from a seeded human induced pluripotent stem cell (iPSC)-derived NPCs under an applied EF. [316] Despite this advantage over electrical stimulation through 2D films, cell viability was reduced on 3D scaffolds. More recently, tubular guidance scaffolds based on PPy have been fabricated with more complex geometries, such as single-and multichanneled tubes, and shown to support sciatic nerve regeneration in rats.…”

Section: Polypyrrole (Ppy)mentioning

confidence: 99%

“…[ 325 ] Blending of PANI with PCL has resulted in biodegradable scaffolds with relatively high conductivity (4.2 S cm −1 ). [ 326 ] Applied electrical stimulation to PC12 cells seeded onto this PANI/PCL scaffold increased cell proliferation and expression of neurotrophic factors; however, it is not clear how these measures compare to PC12 cells on standard culture substrates. Furthermore, PC12 cells derived from rat pheochromocytomas outside of the CNS are unlikely to behave as human NS/PCs.…”

Section: Electrically Conductive Polymers (Cps)mentioning

confidence: 99%

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Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells

Bierman-Duquette

Safarians

Huang

et al. 2021

Adv Healthcare Materials

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Conductive biomaterials provide an important control for engineering neural tissues, where electrical stimulation can potentially direct neural stem/progenitor cell (NS/PC) maturation into functional neuronal networks. It is anticipated that stem cell-based therapies to repair damaged central nervous system (CNS) tissues and ex vivo, "tissue chip" models of the CNS and its pathologies will each benefit from the development of biocompatible, biodegradable, and conductive biomaterials. Here, technological advances in conductive biomaterials are reviewed over the past two decades that may facilitate the development of engineered tissues with integrated physiological and electrical functionalities. First, one briefly introduces NS/PCs of the CNS. Then, the significance of incorporating microenvironmental cues, to which NS/PCs are naturally programmed to respond, into biomaterial scaffolds is discussed with a focus on electrical cues. Next, practical design considerations for conductive biomaterials are discussed followed by a review of studies evaluating how conductive biomaterials can be engineered to control NS/PC behavior by mimicking specific functionalities in the CNS microenvironment. Finally, steps researchers can take to move NS/PC-interfacing, conductive materials closer to clinical translation are discussed.

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Section: Porous 3d and Tubular Scaffoldsmentioning

confidence: 93%

Section: Icr Mouse (1 D Postnatal) Hippocampal Nscsmentioning

confidence: 99%

Section: Polypyrrole (Ppy)mentioning

confidence: 99%

Section: Electrically Conductive Polymers (Cps)mentioning

confidence: 99%

See 2 more Smart Citations

Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells

Bierman-Duquette

Safarians

Huang

et al. 2021

Adv Healthcare Materials

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show abstract

“…Nowadays, many researchers use conductive materials to combine electrical signals with physical and mechanical cues to improve the adhesion, proliferation, migration, and differentiation of stem cells in neural tissue engineering. There are numerous fillers to fabricate electrical or ionic conductive polymers, including different types of carbon, intrinsic conductive polymers, and some polar polymers [30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Ionic conductive nanocomposite based on poly(l-lactic acid)/poly(amidoamine) dendrimerelectrospun nanofibrous for biomedical application

Memarian¹,

Solouk²,

Bagher³

et al. 2021

Biomed. Mater.

Self Cite

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The modification of poly (l-lactic acid) (PLLA) electrospun nanofibrous scaffolds was carried out by blending with second-generation poly amidoamine (PAMAM) for enhancement of their ionic conductivity. The samples containing PLLA and various amounts of PAMAM (1%, 3%, 5%, and 7% by wt.) were fabricated by electrospinning techniques. The electrospun fibers were characterized using scanning electron microscopy (SEM), porosity, Fourier-transform infrared (FTIR) spectroscopy, differential scanning calorimetry, contact angle measurement, water uptake measurement, mechanical properties, and electrical properties. Furthermore, in vitro degradation study and cell viability assay were investigated in biomaterial applications. Creating amide groups through aminolysis reaction was confirmed by FTIR analysis successfully. The results reveal that adding PAMAM caused an increase in fiber diameter, crystallinity percentage, hydrophilicity, water absorption, elongation-at-break, and OE-mesenchymal stem cell viability. It is worth mentioning that this is the first report investigating the conductivity of PLLA/PAMAM nanofiber. The results revealed that by increasing the amount of PAMAM, the ionic conductivity of scaffolds was enhanced by about nine times. Moreover, the outcomes indicated that the presence of PAMAM could improve the limitations of PLLA like hydrophobicity, lack of active group, and poor cell adhesion.

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Porous Scaffolds Made From Starch Nanoparticle‐Stabilized Medium Internal Phase Emulsion Polymerization: Effect of Pore Size and Polyaniline on Cell Growth

Kavousi,

Akbari‐Birgani,

Pour‐Esmaeil

et al. 2024

Polymers for Advanced Techs

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Polymeric three‐dimensional (3D) porous scaffolds were prepared through medium internal phase emulsion (MIPE) templating. Their potential applicability as substrates for culturing human breast cancer (MCF‐7) cells and human neuroblastoma (SH‐SY5Y) cells was investigated. The crosslinked starch nanoparticle‐stabilized oil‐in‐water MIPEs containing acrylamide/methylenebisacrylamide/poly(ethylene oxide) (PEO) in the continuous phase cured in 1 and 6 h to produce 3D porous scaffolds called, respectively, PolyMIPE‐SP and PolyMIPE‐SP after the removal of the internal oil phase. Field emission scanning electron microscopy (FE‐SEM) micrographs revealed uniform interconnected porous structures with average pore sizes of 46.74 and 7.13 μm for PolyMIPE‐LP and PolyMIPE‐SP, respectively. Cell viability and cell culture studies exhibited that the biocompatibility, proliferation, and growth of SH‐SY5Y and MCF‐7 cells on PolyMIPE‐LP were better than those on PolyMIPE‐SP due to the proper placement of cells in large pores. Then, deposited conductive polyaniline (PANI) nanoparticles on the pore surface of PolyMIPE‐LP induced a rough surface with a nanoprotrusion structure and balanced hydrophobicity/hydrophilicity, enhancing cell growth in the scaffold. The cyclic voltammograms revealed that the conductivity of PolyMIPE‐LP/PANI scaffolds depends directly on the swelling degree of the scaffold. Consequently, these findings make PolyMIPE‐LP/PANI a suitable bioactive substrate for electroactive cell studies, chemo‐photothermal therapy of cancer cells, and drug testing platforms.

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Synthesis and characterization of conductive neural tissue engineering scaffolds based on urethane-polycaprolactone

Cited by 11 publications

References 49 publications

Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells

Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells

Ionic conductive nanocomposite based on poly(l-lactic acid)/poly(amidoamine) dendrimerelectrospun nanofibrous for biomedical application

Porous Scaffolds Made From Starch Nanoparticle‐Stabilized Medium Internal Phase Emulsion Polymerization: Effect of Pore Size and Polyaniline on Cell Growth

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