Synthesis and characterization of S-PCL-PDMAEMA for co-delivery of pDNA and DOX

Lo, Yu-Lun; Chen, Guojing; Feng, Tzu-Hwa; Li, Ming-Han; Wang, Lifang

doi:10.1039/c3ra46914j

Cited by 25 publications

(22 citation statements)

References 40 publications

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“…Although researches recently show that polymer materials containing segments of PCL and PDMAEMA can be potentially used in drug delivery applications [13,15,[23][24][25][26][27][28], there is not a general consensus about the effect of their structure on their performance. The aim of this study is to establish relationships between the composition and molecular weight of triblock copolymers PDMAEMA-b-PCL-b-PDMAEMA with the colloidal properties of micelles obtained from their assembly in aqueous medium.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and micellization properties of triblock copolymers PDMAEMA-b-PCL-b-PDMAEMA and their applications in the fabrication of amphotericin B-loaded nanocontainers

Diaz

Pérez

2014

Colloid Polym Sci

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In this study, we report the synthesis of PDMAEMA-b-PCL-b-PDMAEMA via ATRP starting from two dibromide-end polycaprolactone (PCL) of 2 and 10 kDa. The copolymerization was confirmed by nuclear magnetic resonance and gel permeation chromatography. The micellar properties of copolymers with different compositions were studied at pH 5.0, 6.0, 7.0, and 7.5. According to results, properties such as critical micellar concentration (CMC), hydrophobicity of micelle cores, and particle size strongly depend on the length of PCL. The pH shows an important effect on the size of the colloidal aggregates. Micelles obtained from copolymers with the lowest polymerization degree of both segments showed to be more appropriate for the encapsulation of amphotericin B (AmB).

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Section: Introductionmentioning

confidence: 99%

Synthesis and micellization properties of triblock copolymers PDMAEMA-b-PCL-b-PDMAEMA and their applications in the fabrication of amphotericin B-loaded nanocontainers

Diaz

Pérez

2014

Colloid Polym Sci

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“…PCL is a highly-crystallized polymer and its crystallinity increases with increasing molecular weight. This fact slows the drug release [ 22 ]. Moreover, the molecular weight of PCL of ca.…”

Section: Resultsmentioning

confidence: 99%

“…Poly( ε -caprolactone) (PCL) is an US Food and Drug Administration (FDA) approved hydrophobic polymer. It is biocompatible, biodegradable nontoxic and widely used in various biomedical applications [ 21 , 22 ]. Compared with poly (lactic acid) (PLA) and poly (glycolic acid) (PGA), PCL-based polymers have excellent properties due to its crystallinity and hydrophobicity [ 23 ], and its degradation products do not substantially lead into acidic environments [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Amphiphilic, Biodegradable, Biocompatible, Thermo-Responsive ABA Triblock Copolymers Based on PCL and PEG Analogues via a Combination of ROP and RAFT: Synthesis, Characterization, and Sustained Drug Release from Self-Assembled Micelles

Wang

Shang

et al. 2018

Polymers

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Well-defined novel, linear, biodegradable, amphiphilic thermo-responsive ABA-type triblock copolymers, poly[2-(2-methoxyethoxy) ethyl methacrylate-co-oligo(ethylene glycolwere synthesized via a combination of ring-opening polymerization (ROP) of ε-caprolactone (εCL) and reversible addition-fragmentation chain transfer polymerization (RAFT) of MEO 2 MA and OEGMA comonomers. The chemical structures and compositions of these copolymers were characterized using Fourier transform infrared spectroscopy (FT-IR) and proton nuclear magnetic resonance ( 1 H NMR). The molecular weights of the copolymers were obtained using gel permeation chromatography (GPC) measurements. Thermo-responsive micelles were obtained by self-assembly of copolymers in aqueous medium. The temperature sensitivity and micelllization behavior of amphiphilic triblock copolymers solutions were studied by transmittance, fluorescence probe, surface tension, dynamic light scattering (DLS) and transmission electron microscopy (TEM). A hydrophobic drug, anethole, was encapsulated in micelles by using the dialysis method. The average particle sizes of drug-loaded micelles were determined by dynamic light scattering measurement. In vitro, the sustained release of the anethole was performed in pH 7.4 phosphate-buffered saline (PBS) at different temperatures. Results showed that the triblock copolymer's micelles were quite effective in the encapsulation and controlled release of anethole. The vial inversion test demonstrated that the triblock copolymers could trigger the sol-gel transition which also depended on the temperature, and its sol-gel transition temperature gradually decreased with increasing concentration. The hydrogel system could also be used as a carrier of hydrophobic drugs in medicine.

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“…111 In fact, this is the most investigated biomedical application for star BCPs prepared by combination of ROP and ATRP, with several examples describing the encapsulation of hydrophobic molecules, such as doxorubicin (DOX) or paclitaxel (PTX), or even genetic material by different core-shell systems for anticancer therapy. [112][113][114][115][116][117][118][119][120][121] For example, Li and co-workers synthesized 3-and 6-arm PCL-b-PHEMA (PHEMA: Poly(hydroxyethyl methacrylate)) star BCPs by "core-first," starting with the ROP of CL followed by the ATRP of HEMA, as effective carriers for PTX delivery. 113 Results showed that drug loading and drug encapsulation efficiencies of the micelles formed in aqueous medium were influenced by both the number of arms and composition of the stars.…”

Section: Degradable Star Bcps For Drug Deliverymentioning

confidence: 99%

Amphiphilic well‐defined degradable star block copolymers by combination of ring‐opening polymerization and atom transfer radical polymerization: Synthesis and application as drug delivery carriers

Oliveira

Mendonça

Simões

et al. 2021

Journal of Polymer Science

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Atom transfer radical polymerization (ATRP) is one of the most popular advanced polymerization techniques in macromolecular science, allowing the synthesis of tailor-made polymers with controlled molecular weight, architecture, composition, and functionality. The combination of ATRP and ringopening polymerization (ROP) provides a straightforward route for the preparation of polymers exhibiting both targeted and well-defined features and biodegradability, which is very interesting for the development of new materials for biomedical applications. Among the different types of polymer architectures, amphiphilic star block copolymers (BCPs) represent a very attractive one, due to their high degree of functionality at the molecular surface, low hydrodynamic volume and higher encapsulation ability, compared to molecular systems based on linear polymers. This review article highlights the research focused on the synthesis of amphiphilic well-defined degradable star BCPs by combination of ROP and ATRP, with particular focus on the development of polymers for biomedical applications, such as anticancer drug delivery, diagnosis therapy, or photodynamic therapy, which is the most investigated field regarding these polymers.

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Synthesis and characterization of S-PCL-PDMAEMA for co-delivery of pDNA and DOX

Abstract: Similar numbers of repeating units of PCL and pDMAEMA were controlled between L-PCL-PDMAEMA and S-PCL-PDMAEMA. DOX-loaded S-PCL-PDMAEMA micelles showed a better cell-killing effect than DOX-loaded L-PCL-PDMAEMA in four cell lines. The co-delivery of DOX and pDNA was confirmed by CLSM.

Cited by 25 publications

References 40 publications

Synthesis and micellization properties of triblock copolymers PDMAEMA-b-PCL-b-PDMAEMA and their applications in the fabrication of amphotericin B-loaded nanocontainers

Synthesis and micellization properties of triblock copolymers PDMAEMA-b-PCL-b-PDMAEMA and their applications in the fabrication of amphotericin B-loaded nanocontainers

Novel Amphiphilic, Biodegradable, Biocompatible, Thermo-Responsive ABA Triblock Copolymers Based on PCL and PEG Analogues via a Combination of ROP and RAFT: Synthesis, Characterization, and Sustained Drug Release from Self-Assembled Micelles

Amphiphilic well‐defined degradable star block copolymers by combination of ring‐opening polymerization and atom transfer radical polymerization: Synthesis and application as drug delivery carriers

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