Synthesis and Characterization of some Novel Quinoxaline-2, 3-Dione Derivatives: A Preliminary Investigation on their Activity Against a Human Epithelial Carcinoma Cell Line

Jubie, Selvaraj; Rajamanickam, Gayathri D.; Srividya, Ammayappan Rajam; Rajagopal, Kalirajan; Prabitha, Prabakaran; Sankar, S.; Elango, K

doi:10.2174/157018011794839385

Cited by 10 publications

(4 citation statements)

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“…The total cell protein content was determined by sulphoradamine B (SRB) assay [ 19 ]. The monolayer cell culture was trypsinized and the cell count adjusted to 1.0 x 10 5 cell/ml using medium (MEM) containing 10 % newborn calf serum.…”

Section: Methodsmentioning

confidence: 99%

Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect

Selvaraj¹,

Dhanabal²,

Chaitanya³

2015

BMC Complement Altern Med

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BackgroundIsolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines.MethodsGamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as its methyl ester. The isolated methyl gamma linolenate was characterized by IR, 1H NMR, 13C NMR and mass spectral analysis and the data were consistent with the structure.ResultsThe percentage yield of isolated methyl gamma linolenate is found to be 71 % w/w, which is a very good yield in comparison to other conventional methods. It was subjected to in-vitro cytotoxic screening on A-549 lung cancer cell lines using SRB assay and result was compared with standard rutin.ConclusionIt may be concluded that the Flash chromatography system plays a major role in improving the yield for theisolation of methyl gamma linoleate from Spirulina platensis and the isolated molecule is a potent cytotoxicagent towards human lung carcinoma cell lines, however it may be further taken up for an extensive study.

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Section: Methodsmentioning

confidence: 99%

Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect

Selvaraj¹,

Dhanabal²,

Chaitanya³

2015

BMC Complement Altern Med

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“…The total cell protein content was determined by Sulphoradamine B (SRB) assay [12,13]. The monolayer cell culture was trypsinized and the cell count adjusted to 1.0 × 10 5 cell/mL using medium (MEM) containing 10% new born calf serum.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and characterization of some novel fatty acid analogues: A preliminary investigation on their activity against human lung carcinoma cell line

et al. 2013

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BackgroundPreparation of some novel heterocyclic compounds with long alkyl and alkenyl chain of cytotoxic activity.MethodsGamma linolenic acid, a poly unsaturated fatty acid and stearic acid, a saturated fatty acid were isolated from the microalga Spirulina platensis. Some novel gamma linolenic acid and stearic acid analogues having 1,3,4-oxadiazole and 1,2,4-triazole were synthesized and characterized by IR, 1H NMR, 13C NMR and mass spectral analysis. Cytotoxicity of these compounds was evaluated by the growth inhibition of A-549 cells in-vitro.ResultsCompound 1 and 3 showed comparable cytotoxicity against the human lung carcinoma A-549 cell lines.

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“…The cytotoxicity screening was performed according to the previous reported method (Jubie et al, 2011a) .…”

Section: Biological Assaymentioning

confidence: 99%

Synthesis and evaluation of coumate analogues as estrogen receptor inhibitors for breast cancer therapy

Selvaraj¹,

Basheer²,

Yadav³

et al. 2019

ijrps

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A sulphatase inhibitor 667 COUMATE is in clinical trials for estrogen-positive breast cancer therapy for postmenopausal women, while there are a number of similar sulphatase inbitors are under development. Schiff's bases are versatile pharmacophores in which the N atom involves in hydrogen bonding with active cell centers interfering in normal cell biology. A library of novel coumate analogues with Schiff bases were designed, and structural based drug design was performed with human estrogen receptor (PDB ID: 2IOG) (Already reported). Based on the in-silico outcomes, seven coumate-schiff bases were synthesized. The compounds were obtained in good yield. The novelty had been ascertained by sci inder software. The synthesized molecules were consistent with their assigned spectra such as IR, Mass and NMR spectral data which con irmed their formation. The cytotoxicity study was performed by MTT assay for all the synthesized compounds. Most of the compounds have good IC 50 values (below 100 µg/ml).Two of the synthesized compounds COU-2 and COU-5 have shown good IC 50 values such as 19µg/ml and 39µg/ml, respectively. They suppressed the proliferation of estrogen receptor overexpressed MCF-7 cells.

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Synthesis and Characterization of some Novel Quinoxaline-2, 3-Dione Derivatives: A Preliminary Investigation on their Activity Against a Human Epithelial Carcinoma Cell Line

Cited by 10 publications

References 0 publications

Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect

Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect

Synthesis and characterization of some novel fatty acid analogues: A preliminary investigation on their activity against human lung carcinoma cell line

Synthesis and evaluation of coumate analogues as estrogen receptor inhibitors for breast cancer therapy

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