Synthesis and characterization of Zn-Doped hydroxyapatite: scaffold application, antibacterial and bioactivity studies

Abstract

In this study, the antimicrobial and scaffold of zinc-substituted hydroxyapatite, (Zn-HAp) synthesized via chemical co-precipitation technique was investigated. The structure of the synthesized Zn-HAp was investigated with X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Scanning electron microscope (SEM), Energy dispersive X-spectroscopy (EDAX), transmission electron microscope (TEM), Thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS). Bioactivity study was performed in simulated body fluid (SBF), while the antimicrobial activity was studied using disc diffusion method. The XRD structure revealed that Zn ion incorporation up to 10% led to the second phase hydroxyapatite (HAp) formation, while higher concentration diminished the apatite structure. The presence of phosphate ions, carbonates ions, and hydroxyl groups in the apatite powder was ascertained by the FT-IR evaluation. SEM evaluation showed that the apatite contains fine particles with nearly round shape with interconnected pores and decreasing Ca/P ratio with increasing Zn ion concentration. TEM results showed particulate polycrystalline apatite with crystallite size ranging from 68 nm in pure HAp to 41 nm in 20% Zn-doped HAp indicating a decrease in the crystal size with increasing Zn ion in the samples. The bioactivity study showed spherical deposition around the porous region of the scaffold HAp suggesting the growth of apatite in SBF media after 7 days of incubation, while antibacterial activity studies showed zones of inhibition with an increase in zinc ions concentrations.

Osteogenic potential of human mesenchymal stem cells on eggshells‐derived hydroxyapatite nanoparticles for tissue engineering

“…However, after a certain time interval (60 min) no further enhancement in the intensity of these peaks was observed, indicating that 60 min is the optimum time for the reaction via the sonication process. The appearance of the broad absorption peaks between 3,483 and 3,561 cm −1 indicates the moisture moiety (Figure a; Ofudje, Adeogun, Idowu, & Kareem, ; Ofudje et al, ). The existence of the absorption peaks in FTIR spectra at 1031, 949, 600, 563, and 516 cm −1 suggests the existence of phosphate moiety in the synthesized powder (Nasiri‐Tabrizi, Fahami, & Ebrahimi‐Kahrizsangi, ).…”

Fabrication of Silver- and Zinc-Doped Hydroxyapatite Coatings for Enhancing Antimicrobial Effect

“…Recently, in their study, van Hengel et al [4] have shown that surfaces functionalized with zinc and silver ions exhibit antimicrobial activity, even against bacterial strains of methicillin-resistant Staphylococcus aureus (MRSA). The use of metallic ions (Silver and Zinc) as antimicrobial agents give to hydroxyapatite the ability to fight against gram-positive (Staphylococcus aureus, Streptococcus mutans, Bacillus cereus) [1,7,17,18], gram-negative (Escherichia coli, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum) [7,10] bacterial strains and fungi (Candida albicans) [1].…”

<p>Advanced Mg, Zn, Sr, Si Multi-Substituted Hydroxyapatites for Bone Regeneration</p>

“…Due to Zn beneficial effects on bone formation, the synthesis of substituted HAP with Zn (HAP-Zn) has been carried out by using chemical precipitation methods for different Zn substitution levels into the HAP lattice. 16,[47][48][49][50] Silicon, Si, was found to be essential in bone growth and development. 51 This discovery has inspired the development of bioceramics based on Si-substituted hydroxyapatite (HAP-Si), [52][53][54][55][56][57] primarily by using precipitation routes.…”