This research includes synthesis of new heterocycles containing disubstituted Tetrazole derivatives. Imine compounds were synthesized by reaction of primary aromatic amines with various substituted benzaldehydes in the presence of glacial acetic acid as catalyst in absolute ethanol to obtain new imine compounds O 1-O 5. The novel five-membered heterocycles as Tetrazole derivatives O 6-O 10 were obtained from treatment of each new imine compounds with sodium azide compound. Newly synthesized compounds were identified via spectral methods (FT-IR, 1 H-NMR and 13 C-NMR) spectra and some physical properties. O 6 is the best derivative that has significantly (p<0.01) recorded a stronger influence to inhibit the growth of Candida guilliermondii at an average of the zone of inhibition 14.0 mm. While, O 9 derivative recorded the lowest zone of inhibition 7.3 mm toward the same clinical fungal pathogen. The present work may be helpful in designing more potential antibacterial and antifungal agents for therapeutic use in the future.