2010
DOI: 10.1016/j.procbio.2010.05.004
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Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11

Abstract: a b s t r a c tWe produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activity per gram of dry solids at 96 h. This fermented solid was lyophilized and delipidated, packed into a column and used to catalyze the synthesis of biodiesel through the ethanolysis of soybean oil in … Show more

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Cited by 104 publications
(60 citation statements)
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“…To achieve this, they suggested an injection of cooldry air and replacing moisture at different points in the packedbed. Salum et al [108] cultivated Burkholderia cepacia LTEB11 on a mixture of sugarcane bagasse and sunflower seed meal. They found that the fermentation solid could be used to catalyse the ethanolysis of soybean oil to produce biodiesel in a fixed packed-bed bioreactor (a co-solvent-free system).…”
Section: Groupmentioning
confidence: 99%
“…To achieve this, they suggested an injection of cooldry air and replacing moisture at different points in the packedbed. Salum et al [108] cultivated Burkholderia cepacia LTEB11 on a mixture of sugarcane bagasse and sunflower seed meal. They found that the fermentation solid could be used to catalyse the ethanolysis of soybean oil to produce biodiesel in a fixed packed-bed bioreactor (a co-solvent-free system).…”
Section: Groupmentioning
confidence: 99%
“…In this context, the manufacturing of lipases by SSF is an interesting approach to decrease the enzyme price and may be used to make commodities like biodiesel, which is achieved by a more price competitive enzymatic route. After fermentation, the biocatalyst can be extracted from the solid medium for further use or, as reported recently, the biodiesel synthesis reactions can be performed by the direct addition of the fermented solid into the reaction medium [160][161][162][163][164]. Recently, Aguieiras et al [165] studied a two enzyme hydroesterification strategy to synthetize biodiesel employing cheap biocatalysts in both reactions.…”
Section: Biodieselmentioning
confidence: 99%
“…Lipases can esterify the free fatty acids contained in frying oils and animal fats, which allow being more technically feasible than chemical route. Additionally, glycerol from biodiesel production can be easily recovered without complex treatment, the enzyme may be recycled, and the process requires less energy and has significant reduction in the amount of waste generated [36,37].…”
Section: Biodiesel Production By Enzymatic Routementioning
confidence: 99%