Synthesis of inorganic materials with complex form

Mann, Stephen; Ozin, Geoffrey A.

doi:10.1038/382313a0

Cited by 1,143 publications

(702 citation statements)

References 38 publications

Supporting

Mentioning

685

Contrasting

Unclassified

Order By: Relevance

“…Living organisms produce complex composite materials that exhibit an amazing hierarchical assembly of bioorganic molecules and inorganic ions ranging from the nano-to macroscopic scale (Mann & Ozin 1996;Addadi & Weiner 1997;Pouget et al 2007). These highly organized structures often exhibit excellent physical and/or chemical properties that outperform artificial materials, and, in comparison with the usual synthetic methods, the intricate architectures of these biominerals can be formed under conditions that are incredibly mild.…”

Section: Overviewmentioning

confidence: 99%

Formation of magnetite by bacteria and its application

Arakaki

Nakazawa

Nemoto

et al. 2008

J. R. Soc. Interface.

220

176

View full text Add to dashboard Cite

Magnetic particles offer high technological potential since they can be conveniently collected with an external magnetic field. Magnetotactic bacteria synthesize bacterial magnetic particles (BacMPs) with well-controlled size and morphology. BacMPs are individually covered with thin organic membrane, which confers high and even dispersion in aqueous solutions compared with artificial magnetites, making them ideal biotechnological materials. Recent molecular studies including genome sequence, mutagenesis, gene expression and proteome analyses indicated a number of genes and proteins which play important roles for BacMP biomineralization. Some of the genes and proteins identified from these studies have allowed us to express functional proteins efficiently onto BacMPs, through genetic engineering, permitting the preservation of the protein activity, leading to a simple preparation of functional protein-magnetic particle complexes. They were applicable to high-sensitivity immunoassay, drug screening and cell separation. Furthermore, fully automated single nucleotide polymorphism discrimination and DNA recovery systems have been developed to use these functionalized BacMPs. The nano-sized fine magnetic particles offer vast potential in new nano-techniques.

show abstract

Section: Overviewmentioning

confidence: 99%

Formation of magnetite by bacteria and its application

Arakaki

Nakazawa

Nemoto

et al. 2008

J. R. Soc. Interface.

220

176

View full text Add to dashboard Cite

show abstract

“…[6][7][8][9][10][11][12][13] However, it is still challenging to manipulate their self-assembly in a controlled way and to analyze their assembled products precisely at the molecular level. 14,15 In this study, we have generated two different individual mutants of a protein cage with functional groups either inside or outside of the cage ( Figure 1A).…”

mentioning

confidence: 99%

Controlled Assembly of Bifunctional Chimeric Protein Cages and Composition Analysis Using Noncovalent Mass Spectrometry

et al. 2008

View full text Add to dashboard Cite

Protein cage architectures, such as viral capsids, ferritins, and heat shock proteins (Hsp), have been extensively used as model systems to study the self-assembly of macromolecular complexes [1][2][3][4][5] and as nanoreactors for materials synthesis. [6][7][8][9][10][11][12][13] However, it is still challenging to manipulate their self-assembly in a controlled way and to analyze their assembled products precisely at the molecular level. 14,15 In this study, we have generated two different individual mutants of a protein cage with functional groups either inside or outside of the cage ( Figure 1A). We chemically modified different cages and reconstructed chimeric cages with a controlled ratio of two subunit types ( Figure 1B). Using mass spectrometry, we were able to determine the compositions of the ensemble population and also of the individual chimeric cages within the population at the molecular level. A model based on a binomial distribution suggested chimeric cages are assembled by random incorporation of the two individual subunits.Mass spectrometry has been used to monitor multicomponent systems, because it can simultaneously resolve individual molecular masses present in a mixture. [16][17][18] Using a combination of electrospray ionization (ESI) 19 and a time-of-flight (TOF) mass analyzer, it is possible to determine the masses of individual protein components of a noncovalently associated macromolecular complex as well as the mass of the intact macromolecular complex without disturbing the structures. 16,[20][21][22][23] The Dps (DNA binding protein from starved cells) from the Gram-positive bacterium Listeria innocua (Li) is a member of the ferritin superfamily and prevents oxidative damage of DNA by accumulating iron atoms within its central cavity to produce an iron oxide core similar to that of ferritins. 24,25 The LiDps consists of 12 identical 18 kDa subunits that self-assemble into a hollow protein cage having tetrahedral 23 symmetry ( Figure 1A). 24 The LiDps has an outer diameter of 9 nm and an inner cavity diameter of 5 nm with 0.8 nm pores at the 3-fold axis where molecules can pass through to the interior ( Figure 1A). 24 The LiDps has been used as a template for nanomaterials synthesis of metal oxides of iron 26 and cobalt 27 as well as cadmium sulfide 28 and platinum 29 with or without modifications. The small number of subunits, robustness at high temperature, 26,27 and intrinsic biomineralizing capability 25 of the LiDps protein cage make it an attractive modifiable nanoreactor for nanomaterials syntheses. In addition, the defined small cavity size 24 allows synthesis of extremely small nanostructured materials. 29 Two individual cysteine mutants, one exposed on the interior surface and the other on the exterior surface, were generated to adapt the LiDps for selective chemical modifications. The serine residue at position 138 located in the middle of helix E where it is directed toward the inside cavity was substituted with cysteine (S138C) 29 ( Figure 1A, blue). Alternatively, for th...

show abstract

“…Calcite microcrystals have been grown on a wide variety of templates, including Kevlar, [54][55][56] nylon, [57] self-assembled monolayers, proteins, etc. [51,[58][59][60][61][62][63][64][65][66][67][68] Reminiscent of collagen processing, [69] the approach delineated herein represents an example of hierarchical self-assembly in which each stage involves building blocks of increasing size such that the resulting supramolecular structure spans over six orders of magnitude, from nanometer-sized covalent building blocks to millimeter calcite wires. [70] We have demonstrated that streptavidin (the linker) combined with a linear tetrabiotinylated connector, the [Fe(Biot 2 -terpy) 2 ] 2+ complex, spontaneously self-assemble into a one-dimensional metal-organic protein framework.…”

mentioning

confidence: 99%