Synthesis of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate)/chitosan/silver nanocomposite material with enhanced antimicrobial activity

Abstract

This work aims to shed light in the fabrication of poly(3-hydroxybutyrate-co-44%-4-hydroxybutyrate)[P(3HB-co-44%4HB)]/chitosan-based silver nanocomposite material using different contents of silver nanoparticle (SNP); 1-9 wt%. Two approaches were applied in the fabrication; namely solvent casting and chemical crosslinking via glutaraldehyde (GA). A detailed characterization was conducted in order to yield information regarding the nanocomposite material. X-ray diffraction analysis exhibited the nature of the three components that exist in the nanocomposite films: P(3HB-co-4HB), chitosan, and SNP. In term of mechanical properties, tensile strength, and elongation at break were significantly improved up to 125% and 22%, respectively with the impregnation of the SNP. The melting temperature of the nanocomposite materials was increased whereas their thermal stability was slightly changed. Scanning electron microscopy images revealed that incorporation of 9 wt% of SNP caused agglomeration but the surface roughness of the material was significantly improved with the loading. Staphylococcus aureus and Escherichia coli were completely inhibited by the nanocomposite films with 7 and 9 wt% of SNP, respectively. On the other hand, degradation of the nanocomposite materials outweighed the degradation of the pure copolymer. These bioactive and biodegradable materials stand a good chance to serve the vast need of biomedical applications namely management and care of wound as wound dressing.

Facile fabrication of polyethylene/silver nanoparticle nanocomposites with silver nanoparticles traps and holds early antibacterial effect

“…In terms of antibacterial packaging applications, this may have been significant for the early prevention of certain pathogenic bacteria with AgNP‐containing nanocomposites. High amounts of AgNPs at 5 and 9 wt % in other related PE and poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate)/chitosan nanocomposites prevented E. coli growth up to 99% in comparison to the neat PE after 24 h . Salmonella and Bacillus species were strongly inhibited in the presence of AgNP with an amount of approximately 1.2 wt % in a gelatin film and epoxy/clay nanocomposites .…”

Low molecular weight chitosan-coated silver nanoparticles are effective for the treatment of MRSA-infected wounds

“…Human fibroblast HS27 cells were from Shanghai Bioleaf Biotech (Shanghai, People's Republic of China) and cultured in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/mL streptomycin under a humidified 5% CO 2 Detection of cell apoptosis assay by hoechst/propidium iodide staining Human fibroblast HS27 cells were cultured to 80% confluence and treated with 30 μg/mL of three types of AgNPs for 24 hours. After washing three times with phosphatebuffered saline, the cells were added to 10 μL Hoechst 33258 (5 μg/mL) and incubated at 37°C for 10 minutes, followed by adding 5 μL PI (15 μg/mL) in the dark at room temperature for 10 minutes.…”

“…[1][2][3][4] However, the toxicology of AgNPs remains largely unknown. 5,6 Moreover, AgNPs can be absorbed into the bloodstream via different routes of administration, leading to deposition of silver in many organs, including the liver and spleen, and potential organ damage after a certain amount of accumulation.…”

Cell-compatible PHB/silk fibroin composite nanofibermat for tissue engineering applications

“…Moreover, PHB is known to be biocompatible to various cell lines and no report exists of its toxicity in medical applications (Suwantong et al, 2007;da Silva et al, 2016). These properties make it suitable for a variety of applications in the health industry, such as controlled release systems (Daranarong et al, 2014), surgical sutures (Fedorov et al, 2007), wound dressings (Rennukka et al, 2014), and orthopedic uses (Alves et al, 2011). However, the intrinsic brittleness and high cost of PHB limits its use in tissue engineering applications.…”