Synthesis of Stable Silica-Dye Hybrid Nanomaterial as DNA Carrier

Abstract

A new method is proposed for the fabrication of fluorescence-labeled and amine-modified silica nanoparticles for application as nonviral vectors in gene delivery. Highly monodisperse, stable fluorescent silica nanoparticles were prepared using 2,5-bis(5-tert-butyl-2-benzoxazolyl)thiophene and the water-in-oil microemulsion method. The green-fluorescent-protein gene can be easily combined onto the positively charged surfaces of nanoparticles to form a nanoparticle-DNA complex. The nanoparticle-DNA complex successfully passed through various barriers into the HeLa and HEK 293 K cells. The cytotoxicity of the PEI-coated and BBOT-encapsulated silica nanoparticles on both the HeLa and HEK 293T cell lines was found to be at an acceptable level for use as gene carriers when the particle concentration was below 125 microg/ml. The fluorescence intracellular images confirm the successful delivery of the nanoparticle-DNA complex and gene expression. The present work suggests the potential use of dye-incorporated silica nanoparticles in nonviral gene delivery.

Nanomedicine as an innovative therapeutic strategy for pediatric lung diseases

“…43,44 Kumar et al 45 prepared cationic SiO 2 nanoparticles which had a size of 10-100 nm, and this non-toxic material has excellent in vivo gene transfer ability in mouse lung. Kim's group 16 synthesized a stable silica-dye hybrid nanomaterial as DNA carriers. It can successfully deliver DNAs and express a gene.…”

“…If nanoparticles could be delivered to the lungs, they can avoid mucociliary clearance, and the high mass‐to‐surface ratio is helpful for the therapy of lung‐specific diseases and systemic delivery . To be suitable for pulmonary drug delivery, nanocarriers can be synthesized as liposomes, polymer micelles, dendrimers, and inorganic materials, and can be loaded with protein, peptide, DNA, siRNA, antibody, anticancer drugs, and so on. To ensure clinically localized therapy of the target organ and avoid accumulation of nanomaterials in healthy organs, the nanocarriers should be engineered such that they slowly degrade, can react to stimuli and be site specific .…”

Inorganic-Organic Hybrid Nanomaterials for Therapeutic and Diagnostic Imaging Applications

“…SNPs are also promising materials as DNA carriers for gene therapy [ 17 , 23 , 24 ]. Recently, the potential of cationic SNPs was investigated for in vivo gene transfer [ 25 ].…”

Fluorescent Silica Nanoparticles for Medical Imaging

“…2,[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] Other smaller molecules (proteins, antibodies, cofactors) require specialized conjugation to the SiNP. 38,[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] Even the addition of magnetic NP conjugation to the FSNP 3,4,62 greatly assists in the recognition and tracking of different organic molecules.…”