“… 43 Because of the modification of polyethylene glycol (PEG) by the film-forming material, PEG molecules form a protective hydrophilic layer, which helps to avoid recognition by the immune system, thereby reducing the uptake of PEG-coated nanoparticles by macrophages, allowing NPs to have a long circulation time in the body, and reducing the phagocytosis of the reticuloendothelial system in the body. 10 , 44 Through the detection of a series of characterizations of the prepared NPs, we conclude that the NPs we prepared are regularly round in shape, have a small overall particle size, can better circulate in the body and enter the site of tumor tissue, and can perform subsequent in vitro and in vivo performance detection experiments. Figure 1 In vitro characteristics of iRGD-ICG-10-HCPT-PFP-NPs and ICG-10-HCPT-PFP-NPs ( A ) morphology of iRGD-ICG-10-HCPT-PFP-NPs under a light microscope, scale bar: 20 μm ( B ) morphology of iRGD-ICG-10-HCPT-PFP-NPs under the white light of a CLSM, scale bar: 20 μm ( C ) morphology of iRGD-ICG-10-HCPT-PFP-NPs under a red fluorescence channel of a CLSM, scale bar: 100 μm ( D ) structure of iRGD-ICG-10-HCPT-PFP-NPs under TEM, scale bar: 200 nm ( E ) particle size of iRGD-ICG-10-HCPT-PFP-NPs ( F ) zeta potential of iRGD-ICG-10-HCPT-PFP-NPs ( G ) particle size of ICG-10-HCPT-PFP-NPs ( H ) potential of ICG-10-HCPT-NPs.…”
Section: Resultsmentioning
confidence: 88%
“…43 Because of the modification of polyethylene glycol (PEG) by the film-forming material, PEG molecules form a protective hydrophilic layer, which helps to avoid recognition by the immune system, thereby reducing the uptake of PEG-coated nanoparticles by macrophages, allowing NPs to have a long circulation time in the body, and reducing the phagocytosis of the reticuloendothelial system in the body. 10,44 Through the detection https://doi.org/10.2147/IJN.S325891…”
Purpose
Prepare a multifunctional ultrasound molecular probe, cell-penetrating peptide-modified 10-hydroxycamptothecin-loaded phase-transformation lipid nanoparticles (iRGD-ICG-10-HCPT-PFP-NPs), and to combine iRGD-ICG-10-HCPT-PFP -NPs with low-intensity focused ultrasound (LIFU) for precision theranostics against hepatocellular carcinoma (HCC).
Materials and Methods
The morphology of nanoparticles (NPs) and iRGD-ICG-10-HCPT-PFP-NPs was detected. In vitro, we examined targeting ability by flow cytometry and confocal laser scanning microscopy (CLSM), assessed penetration ability into hepatoma cells, and assessed killing ability. In vivo, we examined the targeting ability of the NPs with a photoacoustic (PA) imager and fluorometer (FL), while LIFU irradiation was used to trigger the release of chemotherapeutic drugs, which had a therapeutic effect on tumors.
Results
The particle size of iRGD-ICG-10-HCPT-PFP-NPs was 298.4 ± 10.42 nm. In vitro, iRGD-ICG-10-HCPT-PFP-NPs bound more to SK-Hep1 cells than ICG-10-HCPT-PFP-NPs. iRGD-ICG-10-HCPT-PFP-NPs could achieve PA/ultrasound imaging. The percentage of antiproliferative and apoptotic cells in the iRGD-ICG-10-HCPT-PFP-NPs+LIFU group was significantly higher. In vivo, iRGD-ICG-10-HCPT-PFP-NPs can target tumor sites and achieve PA/ultrasound imaging. The tumor volume in the iRGD-ICG-10-HCPT-PFP-NPs+LIFU group was significantly smaller, and the antiproliferative and proapoptotic effects were higher.
Conclusion
We successfully prepared a novel molecular probe that has good targeting, can perform ultrasound/PA dual-modality imaging, and can penetrate deep into tumors to achieve better therapeutic tumor effects, providing a new idea and method for theranostics of HCC.
“… 43 Because of the modification of polyethylene glycol (PEG) by the film-forming material, PEG molecules form a protective hydrophilic layer, which helps to avoid recognition by the immune system, thereby reducing the uptake of PEG-coated nanoparticles by macrophages, allowing NPs to have a long circulation time in the body, and reducing the phagocytosis of the reticuloendothelial system in the body. 10 , 44 Through the detection of a series of characterizations of the prepared NPs, we conclude that the NPs we prepared are regularly round in shape, have a small overall particle size, can better circulate in the body and enter the site of tumor tissue, and can perform subsequent in vitro and in vivo performance detection experiments. Figure 1 In vitro characteristics of iRGD-ICG-10-HCPT-PFP-NPs and ICG-10-HCPT-PFP-NPs ( A ) morphology of iRGD-ICG-10-HCPT-PFP-NPs under a light microscope, scale bar: 20 μm ( B ) morphology of iRGD-ICG-10-HCPT-PFP-NPs under the white light of a CLSM, scale bar: 20 μm ( C ) morphology of iRGD-ICG-10-HCPT-PFP-NPs under a red fluorescence channel of a CLSM, scale bar: 100 μm ( D ) structure of iRGD-ICG-10-HCPT-PFP-NPs under TEM, scale bar: 200 nm ( E ) particle size of iRGD-ICG-10-HCPT-PFP-NPs ( F ) zeta potential of iRGD-ICG-10-HCPT-PFP-NPs ( G ) particle size of ICG-10-HCPT-PFP-NPs ( H ) potential of ICG-10-HCPT-NPs.…”
Section: Resultsmentioning
confidence: 88%
“…43 Because of the modification of polyethylene glycol (PEG) by the film-forming material, PEG molecules form a protective hydrophilic layer, which helps to avoid recognition by the immune system, thereby reducing the uptake of PEG-coated nanoparticles by macrophages, allowing NPs to have a long circulation time in the body, and reducing the phagocytosis of the reticuloendothelial system in the body. 10,44 Through the detection https://doi.org/10.2147/IJN.S325891…”
Purpose
Prepare a multifunctional ultrasound molecular probe, cell-penetrating peptide-modified 10-hydroxycamptothecin-loaded phase-transformation lipid nanoparticles (iRGD-ICG-10-HCPT-PFP-NPs), and to combine iRGD-ICG-10-HCPT-PFP -NPs with low-intensity focused ultrasound (LIFU) for precision theranostics against hepatocellular carcinoma (HCC).
Materials and Methods
The morphology of nanoparticles (NPs) and iRGD-ICG-10-HCPT-PFP-NPs was detected. In vitro, we examined targeting ability by flow cytometry and confocal laser scanning microscopy (CLSM), assessed penetration ability into hepatoma cells, and assessed killing ability. In vivo, we examined the targeting ability of the NPs with a photoacoustic (PA) imager and fluorometer (FL), while LIFU irradiation was used to trigger the release of chemotherapeutic drugs, which had a therapeutic effect on tumors.
Results
The particle size of iRGD-ICG-10-HCPT-PFP-NPs was 298.4 ± 10.42 nm. In vitro, iRGD-ICG-10-HCPT-PFP-NPs bound more to SK-Hep1 cells than ICG-10-HCPT-PFP-NPs. iRGD-ICG-10-HCPT-PFP-NPs could achieve PA/ultrasound imaging. The percentage of antiproliferative and apoptotic cells in the iRGD-ICG-10-HCPT-PFP-NPs+LIFU group was significantly higher. In vivo, iRGD-ICG-10-HCPT-PFP-NPs can target tumor sites and achieve PA/ultrasound imaging. The tumor volume in the iRGD-ICG-10-HCPT-PFP-NPs+LIFU group was significantly smaller, and the antiproliferative and proapoptotic effects were higher.
Conclusion
We successfully prepared a novel molecular probe that has good targeting, can perform ultrasound/PA dual-modality imaging, and can penetrate deep into tumors to achieve better therapeutic tumor effects, providing a new idea and method for theranostics of HCC.
“…The Zeta potential of the LA-PEG-G aggregates was −35.80 mV in the aqueous solution, which indicated that the surface of the LA-PEG-G aggregates was negatively charged in aqueous solution. A high Zeta potential allows the LA-PEG-G aggregates to maintain good dispersion in aqueous solution [24,25] . The PEG in the LA-PEG-G molecules can help the LA-PEG-G aggregates escape from the trap of Kupffer cells and macrophages in the spleen through scavenger receptors without activating MPS, which help the blood circulation of the LA-PEG-G aggregates in the body [25] .…”
Section: Synthesis Characterization and Aggregationmentioning
LA–PEG–G molecule was prepared through the covalent conjugation of linoleic acid, polyethylene glycol and guanosine. LA–PEG–G molecule can self-assemble into spherical aggregate in aqueous solution. The size of the LA–PEG–G aggregate was approximately 132.1 ± 13.718 nm, and the Zeta potential was −36.3 ± 0.9644 mv. The loading rate of LA–PEG–G aggregates to Dox was 84.62 ± 1.810%. In vitro release experiments showed that Dox release from the LA–PEG–G–Dox nanomedicine was slow-release. Cell experiments showed that LA–PEG–G aggregates have low cytotoxicity, and the relative cancer cells survival rate decreased with the increasing of time and LA–PEG–G–Dox nanomedicine concentration. In vivo experiments showed that LA–PEG–G–Dox nanomedicine can inhibit tumor growth, and the LA–PEG–G aggregate could also delay the tumor growth which is very important to play the synergistic antitumor effect with the anticancer drug. Serum biochemistry showed that LA–PEG–G–Dox nanomedicine which reduced the Dox toxicity had very good antitumor effect. The tumor tissue sections indicated that LA–PEG–G–Dox nanomedicine can effectively induce tumor cell apoptosis, and finally cause tissue necrosis to achieve tumor treatment.
“…The amphiphilic lipopeptides can form aggregates in aqueous solutions, 8 and the water-insoluble small molecule antibiotics can be encapsulated into aggregates to form lipopeptide@antibiotic nanomedicine, which can improve the biological function of water-insoluble small molecule antibiotics, such as the poor solubility, poor bioavailability and short half-life which caused the side effects and limited the therapeutic effects. 9 So, the lipopeptide@antibiotic nanomedicine is also a very promising multifunctional therapy which combines AMPs with bacterial membrane disturbing ability with antibiotics of known mode of action to enhance antibacterial activity, 10 and these multi-mode actions of lipopeptide@antibiotic nanomedicine provide no or minimal chances to develop resistance in these pathogenic bacteria.…”
It is urgent to develop new antimicrobial drugs to overcome bacterial resistance which is a serious threat to human health. Antimicrobial peptides (AMPs) which are ideal substitutes for traditional antibiotics...
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