Synthesis, structure, magnetic and biological activity studies of bis-hydrazone derived Cu(ii) and Co(ii) coordination compounds

Abstract

Four coordination compounds of formulae [Cu(II)2(H2L(1))(HL(1))](ClO4)3·H2O (1), [Cu(II)2(H2L(2))(CH3OH)2](ClO4)2·2CH3OH (2), [Co(II)2(H2L(1))2](ClO4)4 (3) and [Co(II)2(H2L(2))2]·2H2O (4) were synthesized via self-assembly of succinohydrazone derived ligands (H2L(1) = N',N'-4-bis(2-pyridyl)succinohydrazide, H4L(2) = N',N'-4-bis(2-hydroxybenzylidene)succinohydrazide) and Cu(2+) and Co(2+) ions, respectively. The compounds were characterized by crystal structure determination, magnetic measurements and biological activities. Compounds 1, 3 and 4 have discrete double helicate structures, whereas compound 2 is a one-dimensional chain. Magnetic studies show antiferromagnetic exchange interactions in 2 with a J value of -67.1 cm(-1) and antiferromagnetic spin-canting in compound 3 originates through supramolecular H-bonding. For compound 3, a clear bifurcation was observed in zero field cooled (ZFC) and field cooled (FC) measurement at a temperature of 3.5 K and field of 0.1 T, implying long range magnetic ordering below this temperature. Interestingly, all of compounds 1-4 show significant changes in their absorption (hypo- and hyperchromism) in the presence of SS-DNA, inferring interaction between the compounds and DNA. In addition, compounds 1-4 significantly exhibited nuclease activities on both RNA and pUC19 plasmid DNA. Moreover, the nuclease activity was further enhanced in the presence of oxidant (H2O2) and suggests the possible role of reactive oxygen species in DNA nicking ability of compounds 1-4. Furthermore, compounds 1, 2 and 4 exhibited significant cytotoxicity against mammalian cancer cell lines (HeLa, A549 and MDAMB-231). In addition, our results from Annexin/PI staining and DNA fragmentation assays revealed that these compounds are capable of inducing apoptosis and have potential to act as anticancer drugs.

A systematic assessment of chemical, genetic, and epigenetic factors influencing the activity of anticancer drug KP1019 (FFC14A)

“…HeLa cells were procured from ATCC (Manassas, VA, USA) and cultured in standard Dulbecco's Modified Eagles' Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1X solution of antibiotic-antimycotic (Thermo Fisher, India) and maintained at 37°C in a humidified atmosphere containing 5% (v/v) CO 2 . For testing the cytotoxicity (antiproliferative) potential of KP1019, Rapamycin (RAP), and metal cation chlorides (Al 3+ , Ca 2+ , Cd 2+ , Co 2+ , Cu 2+ , Fe 2+ , Mg 2+ , Mn 2+ , Na + , Ni 2+ , and Zn 2+ ) in alone or combination, we performed standard MTT [3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide] cell viability assay as described earlier [ 118 ]. Briefly, HeLa cells were seeded in a 96-well plate at a density of 7000 cells/well and left undisturbed for 24h.…”

Self‐Assembly of Functional Discrete Three‐Dimensional Architectures in Water

“…In contrast to the three-dimensional architectures just covered, M 2 L 3 helicates and flexicates consisting of two metal ions and three or four bridging ligands,typically do not have any significant internal binding space. [100][101][102][103][104] Flexicates are defined as distinct from helicates as they do not possess arigid linker that mechanically couples the chirality at the metal centres.I nstead they employ flexible linkers in combination with stereochemically programmed metal centres to generate diastereomerically pure complexes. [105] Despite the absence of any internal binding space,metalorganic helicates and flexicates,h ave shown promising medicinal properties,insome cases attributed to their ability to bind strongly to DNAa saresult of their complementary size and shape.Like many inorganic complexes with demonstrable medicinal properties,biological studies with helicates and flexicates are frequently performed in aqueous solutions containing trace DMSO or ethanol, and solubility in pure aqueous solution has-to the best of our knowledge-not been reported.…”

“…In contrast to the three‐dimensional architectures just covered, M 2 L 3 helicates and flexicates consisting of two metal ions and three or four bridging ligands, typically do not have any significant internal binding space . Flexicates are defined as distinct from helicates as they do not possess a rigid linker that mechanically couples the chirality at the metal centres.…”

Synthesis, crystal structure, DNA/bovine serum albumin binding and antitumor activity of two transition metal complexes with 4‐acylpyrazolone derivative

“…[32,33] On account of the strong stacking interaction of an aromatic chromophore with the base pairs of DNA, the intercalative mode of binding generally causes hypochromism along with a red shift. [34][35][36][37] Figure 4 presents the absorption spectra of the compounds in the absence or presence of HS-DNA. The absorption spectra of the complexes display two absorption peaks at 245 and 355 nm for complex 1 and at 255 and 341 nm for complex 2.…”