Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures

Abstract

We created nanometer-scale transmembrane channels in lipid bilayers using self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrates and spans a lipid membrane, and a barrel-shaped cap that adheres to the membrane in part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we find similarities to the response of natural ion channels, such as conductances on the order of 1 nS and channel gating. More pronounced gating was seen for mutations in which a single DNA strand of the stem protruded into the channel. In single-molecule translocation experiments, we highlight one of many potential applications of the synthetic channels, namely as single DNA molecule sensing devices.

Application of nucleic acid–lipid conjugates for the programmable organisation of liposomal modules

“…11) [136,137]. Langecker and co-workers designed a DNA origami membrane plug with a central channel that penetrates through the membrane [137].…”

Biomimetic Hybrid Nanocontainers with Selective Permeability

“…The negatively charged DNA pores carry hydrophobic membrane anchors for membrane insertion. DNA pores have so far only been placed into lipid bilayer membranes,6a–6f and it is not known whether they also anchor into polymersomes to form membrane‐puncturing nanosized holes.…”

Beyond the smiley face: applications of structural DNA nanotechnology

“…Transmembrane channel structures in lipid bilayers were created via a transmembrane ‘stem’ that pierced the length of the lipid membrane was created along with a barrel-shaped ‘cap’ [28]. The ‘cap’ was attached to the membrane through 26 cholesterol moieties [28].…”

“…Transmembrane channel structures in lipid bilayers were created via a transmembrane ‘stem’ that pierced the length of the lipid membrane was created along with a barrel-shaped ‘cap’ [28]. The ‘cap’ was attached to the membrane through 26 cholesterol moieties [28]. The physiological functionality of natural ion channels was mimicked, such that conductances of one nanosiemens and channel gating were achieved [28].…”

“…The ‘cap’ was attached to the membrane through 26 cholesterol moieties [28]. The physiological functionality of natural ion channels was mimicked, such that conductances of one nanosiemens and channel gating were achieved [28]. Similarly, a transmembrane molecular valve which controls gated transport of material across a phospholipid bilayer has been created [27].…”