Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes

Datta, Atin R.; Wentz, B A; Shook, Darlene L.; Trucksess, Mary W

doi:10.1128/aem.54.12.2933-2937.1988

Cited by 81 publications

(34 citation statements)

References 16 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, DNA-based assay systems to detect Listeria spp. in food have also been reported (3,9,19,21). However, these techniques take about 2 to 4 days for detection.…”

mentioning

confidence: 99%

Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua

et al. 1991

View full text Add to dashboard Cite

BALB/c mice were immunized with crude cell surface proteins of Listeria monocytogenes V7. Approximately 1,680 hybridomas were generated after two fusions, and the monoclone C1lE9 was selected and used for further characterization. The monoclonal antibody (MAb) produced by CllE9 was immunoglobulin subclass G2b with K light chains. Dot and colony blot results indicated that MAb C11E9 was reactive to all the L. monocytogenes (34 of 34) and Listeria innocua (6 of 6) isolates without any cross-reaction to other organisms tested. Western blot (immunoblot) analysis of crude cell surface proteins in native polyacrylamide gel electrophoresis (PAGE) indicated that MAb Cl1E9 reacts with a single band in each species, with a molecular mass of 174 kDa for L. monocytogenes and 182 kDa for L. innocua. The MAb reacted with one major protein band in Western blot from acid-urea PAGE for both L. monocytogenes and L. innocua. Isoelectric focusing results indicated two immunoreactive protein bands with pls of 8.1 and 7.4 for L. monocytogenes. Sodium dodecyl sulfate (SDS)-PAGE and Western blot analysis indicated several proteins with molecular masses of 76, 66, 56, and 52 kDa for L. monocytogenes and 66, 56, and 52 kDa for L. innocua. Reaction of MAb CllE9 to washed live cells indicated the possible binding of antibody to cell surface antigen. These cell surface antigens could be removed by 1 N HCI plus 9 M urea, 2% SDS-0.5% jI-mercaptoethanol, or 4 M guanidine-HCl. The epitope of MAb C1lE9 binding site was shown to be protein in nature. Periodic acid-Schiff staining and glycoprotein immunoassay indicated that carbohydrate was absent in the epitope. The cellular locations of the MAb C11E9-reactive antigens were calculated to be 76 and 90% outside and 24 and 10% inside the cell membranes of L. monocytogenes and L. innocua, respectively, for 12to 14-h cultures.

show abstract

“…Similarly, DNA-based assay systems to detect Listeria spp. in food have also been reported (3,9,19,21). However, these techniques take about 2 to 4 days for detection.…”

mentioning

confidence: 99%

Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua

et al. 1991

View full text Add to dashboard Cite

show abstract

“…Detection methods for Listeria monocytogenes have been based on genes encoding virulence factors including listeriolysin 0 (Mengaud et al 1988) and invasion-associated protein (Kohler et al 1990). These have used either hybridization probes (Datta et al 1988) or in uitro amplification of fragments by the polymerase chain reaction (PCR) (Furrer et al 1991).…”

mentioning

confidence: 99%

Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction

Powell

Gooding

Garrett

et al. 1994

Lett Appl Microbiol

137

View full text Add to dashboard Cite

show abstract

“…Approaches to the detection of Listeria sp. by such methods have used standard hybridization techniques either to detect genes coding for factors thought to be involved in pathogenicity such as a-haemolysin (Datta et al 1987(Datta et al , 1988, listeriolysin 0 (Mengaud et al 1988), msp (major secreted polypeptide of L. monocytoyenes, Flamm et al 1989) and L. monocytogenes DTH factor (Notermans et al 1989) or to detect specific 16s ribosomal RNA (rRNA) sequences (Klinger et al 1988;Stackenbrandt & large numbers of non-target micro-organisms (Wernars & Notermans 1990).…”

mentioning

confidence: 99%

Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction

Border

Howard

Plastow

et al. 1990

Lett Appl Microbiol

185

110

View full text Add to dashboard Cite

Five oligonucleotide sequences are described that were used as primers in the polymerase chain reaction (PCR) to amplify specific sequences from Listeria DNA. When all five primers were used in combination, three PCR products were possible; a Listeria specific product that occurs with DNA from any Listeria sp., a Listeria monocytogenes specific product that occurs only in the presence of DNA from this organism and a universal product that is found using DNA from any bacterial source. The occurrence of these PCR products was used as a diagnostic test on bacteria isolated from various food samples to detect Listeria sp. and L. monocytogenes.

show abstract

Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes

Cited by 81 publications

References 16 publications

Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua

Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua

Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction

Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction

Contact Info

Product

Resources

About