Systematic characterisation of secondary metabolites from <i>Ixeris sonchifolia</i> by the combined use of HPLC‐TOFMS and HPLC‐ITMS

Shi, Peiying; Zhang, Yufeng; Qu, Haibin; Fan, Xiaohui

doi:10.1002/pca.1253

Cited by 32 publications

(21 citation statements)

References 21 publications

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“…The different processing methods of RA are responsible for the extent of antibacterial activity. In contrast to other processed products of RA, the salt and wheat bran-broiled products display the strongest antibacterial activity (Han et al, 2008;Shi et al, 2011). However, the mechanism of the change of pharmacological effects of processed RA is unknown.…”

Section: Introductionmentioning

confidence: 99%

Determination of the chemical constituents of the different processed products of Anemarrhena asphodeloides Rhizomes by high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Peng

Zhao

Lin

et al. 2015

Biomedical Chromatography

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In this work, high-performance liquid chromatography (HPLC) coupled with a hybrid quadrupole time of-flight mass spectrometry (Q-TOF-MS/MS) was used to study chemical compositions of different processed products of Rhizoma Anemarrhenae (RA). A Grace Alltima(TM) C18 column (250 × 4.6 mm, 5 µm) was used for separation. Mobile phase consisted of 0.1% formic acid and acetonitrile, using gradient elution. ESI-MS data was acquired in both positive and negative mode. The experiment was established on the basis of a series of reference substances (two xanthone and seven saponins) to qualitatively identify the chemical compounds of different processed products of RA by MS analysis. There was no difference in the type of chemical constituents between different processed products of RA. A total of 25 compounds were identified, including four xanthones, 21 steroidal saponins and eight pairs of isomers.

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Section: Introductionmentioning

confidence: 99%

Determination of the chemical constituents of the different processed products of Anemarrhena asphodeloides Rhizomes by high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Peng

Zhao

Lin

et al. 2015

Biomedical Chromatography

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“…The fragment ions of m/z 169, 151 and 125 were generated owing to the loss of C 6 H 10 O 5 , C 6 H 10 O 5 –H 2 O, and C 6 H 10 O 5 –CO 2 from the parent ion. All of the data indicated that compound 1 was gluco‐syringic acid (Shi, Lu, et al, ; Shi, Zhang, et al, ). Compound 2 ( t R = 8.254 min) released an [M − H] − ion of m/z 134.0472.…”

Section: Resultsmentioning

confidence: 99%

“…MS/MS spectral analysis of compound 3 showed that the product ion had an m/z value of 151, which was 18 Da lower than the parent ion, and was formed from the compound of m/z 169 by the loss of H 2 O. In addition, compound 3 showed that another product ion of m/z 125 was formed from the compound of m/z 169 by losing CO 2 (Shi, Lu, et al, ; Shi, Zhang, et al, ). Compound 4 ( t R = 26.106 min) yielded an [M − H] − ion of m/z 353.0874.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, traditional Chinese medicine (TCM) has attracted considerable attention in many countries owing to its high therapeutic effectiveness with few side effects against many diseases (Normile, ; Wu, Ding, Zhu, Yu, & Yan, ). TCM is known for its multiple constituents, exerting pharmacological actions against multiple targets, and is used as a compound mixture for therapy (Hao et al, ; Shi, Lu, et al, ; Shi, Zhang, Fan, & Qu, ; Tong, Dong, Chen, & Zhen, ; Wen et al . , ; Zhang, Ma, Wang, & Wang, ; Zhang, Wang, Ma, Zhu, & Wang, ).…”

Section: Introductionmentioning

confidence: 99%

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Screening free radical scavengers in Xiexin Tang by HPLC‐ABTS‐DAD‐Q‐TOF/MS

Wang

Guo

et al. 2017

Biomedical Chromatography

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Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown that XXT has therapeutic effects on patients with arteriosclerosis owing to its antioxidant activity. However, there is little information about the relationship between the chemical composition of XXT and its antioxidant activity. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in XXT. The 15 compounds identified were gluco-syringic acid, adenine, gallic acid, biflorin, cularine, 6-C-arabinose-8-C-glucose-chrysin, 6-C-glucose-8-C-arabinose-chrysin, baicalin, rhein-8-O-β-d-glucopyranoside, coptisine, epiberberine, jatrorrhizine, norwogonin, 5,7,2'-trihydroxy-6- methoxyflavone and baicalein. In addition, the data showed that the antioxidant activity of peaks 4, 6, and 11 was lower in XXT than in its constituent herbs, while the activity of peaks 1, 2, 3, 5, 7, 8, 10, 12, 13, 14 and 15 was higher in XXT. Compound 5 had the strongest antioxidant activity in XXT, while compound 1 showed the strongest antioxidant activity among its constituent herb. The differences between antioxidant activities of major components of XXT and those of its constituent herbs might be due to the interaction of crude drugs that changes the solubility of active components during the decoction process. The results show that the HPLC-ABTS-DAD-Q-TOF/MS method can successfully combine on-line mass spectrometry with activity detection system. It is a useful tool for the rapid detection and identification of antioxidants, and for quantitative analysis of individual antioxidants in complex mixtures such as plant extracts. Furthermore, this method does not require extensive extract purification and fraction collection.

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“…The chemical compounds of this plant are sesquiterpene lactones, organic acids, flavonoids, and triterpenes [6][7][8][9]. Ixerin Z and 11,13α-dihydroixerin Z are the major sesquiterpene lactones [10], which have been reported to have various activities for instance, anti-inflammatory, anti-microbial, and anti-tumor activities [11,12].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Purification of Sesquiterpene Lactones from <i>Ixeris sonchifolia</i> (Bunge) Hance by High-Speed Counter- Current Chromatography and Semi-Preparative High Performance Liquid Chromatography

Cai¹,

Zhang²,

Li³

et al. 2015

Trop. J. Pharm Res

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Purpose: To isolate and purify sesquiterpene lactones from Ixeris sonchifolia (Bunge) Hance by highspeed counter-current chromatography (HSCCC).Methods: I. sonchifolia was extracted with water and then loaded on a glass column (10 × 1500 cm containing 3000g D101 macroporous resin) where various concentrations of aqueous ethanol (0, 10, 30, 50, and 95 %) were used to elute the column successively. The 50 % ethanol fraction was purified by HSCCC using a solvent system comprised of ethyl acetate : n-butanol: methanol: water (4: 6: 1: 20, v/v) , and semi-preparative high performance liquid chromatography (HPLC). The chemical structures of the components obtained were further confirmed by high-resolution mass spectroscopy (MS) and nuclear magnetic resonance spectroscopy (NMR).Results: Three compounds, including ixerin Z1 (0.7 mg), ixerin Z (11.4 mg),and 11, with purity of 96.2, 98.2, and 98.4 %, respectively, were

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Systematic characterisation of secondary metabolites from Ixeris sonchifolia by the combined use of HPLC‐TOFMS and HPLC‐ITMS

Cited by 32 publications

References 21 publications

Determination of the chemical constituents of the different processed products of Anemarrhena asphodeloides Rhizomes by high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Determination of the chemical constituents of the different processed products of Anemarrhena asphodeloides Rhizomes by high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Screening free radical scavengers in Xiexin Tang by HPLC‐ABTS‐DAD‐Q‐TOF/MS

Isolation and Purification of Sesquiterpene Lactones from <i>Ixeris sonchifolia</i> (Bunge) Hance by High-Speed Counter- Current Chromatography and Semi-Preparative High Performance Liquid Chromatography

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