Systematic toxicity assessment of CdTe quantum dots in Drosophila melanogaster

Nanocomposites have significantly contributed to biomedical science due to less aggregation behavior and enhanced physicochemical properties. This study synthesized a MnFe2O4@poly(tBGE-alt-PA) nanocomposite for the first time and physicochemically characterized it. The obtained hybrid nanomaterial was tested in vivo for its toxicological properties before use in drug delivery, tissue engineering fields, and environmental applications. The composite was biocompatible with mouse fibroblast cells and hemocompatible with 2% RBC suspension. This nanocomposite was tested on Drosophila melanogaster due to its small size, well-sequenced genome, and low cost of testing. The larvae’s crawling speed and direction were measured after feeding. No abnormal path and altered crawling pattern indicated the nonappearance of abnormal neurological disorder in the larva. The gut organ toxicity was further analyzed using DAPI and DCFH-DA dye to examine the structural anomalies. No apoptosis and necrosis were observed in the gut of the fruit fly. Next, adult flies were examined for phenotypic anomalies after their pupal phases emerged. No defects in the phenotypes, including the eye, wings, abdomen, and bristles, were found in our study. Based on these observations, the MnFe2O4@poly(tBGE-alt-PA) composite may be used for various biomedical and environmental applications.

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“…The proportion of surviving flies in each concentration was used to create the graph. 36,37 2.2.9. Average Body Weight Analysis.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Kumar

Bauri

Sahu

et al. 2023

ACS Appl. Bio Mater.

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“…Although the mechanism of genotoxicity has yet to be fully elucidated, induction of oxidative stress caused by ROS buildup is thought to be the foremost source of NP-induced toxicity. Among the possible underlying effect in the toxicity was oxidative stress due to ROS induction (Alaraby et al, 2020;Baeg et al, 2018;Demir, 2020;Paithankar et al, 2022;Priyadarsini et al, 2019). Induced ROS levels trigger inflammation response and DNA and protein damage (Cui et al, 2010;Ng et al, 2017) Several studies concerning boron compounds have revealed that some boron compounds showed genotoxic effects in GADD45a assay (Scott & Walmsley, 2015) and DNA strand breaks (Emanet et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Demir

2022

J of Applied Toxicology

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Boron trioxide nanoparticles (B 2 O 3 NPs) have recently been widely used in a range of applications including electronic device technologies, acousto-optic apparatus fields, and as nanopowder for the production of special glasses. We propose Drosophila melanogaster as a useful in vivo model system to study the genotoxic risks associated with NP exposure. In this study, we have conducted a genotoxic evaluation of B 2 O 3 NPs (size average 55.52 ± 1.41 nm) and its ionic form in D. melanogaster. B 2 O 3 NPs were supplied to third instar larvae at concentrations ranging from 0.1-10 mM. Toxicity, intracellular oxidative stress (reactive oxygen species, ROS), phenotypic alterations, genotoxic effect (via the wing somatic mutation and recombination test, SMART), and DNA damage (via Comet assay) were the end-points evaluated. B 2 O 3 NPs did not cause any mutagenic/recombinogenic effects in all tested non-toxic concentrations in Drosophila SMART. Negative data were also obtained with the ionic form. Exposure to B 2 O 3 NPs and its ionic form (at two highest concentrations, 2.5 and 5 mM) was found to induce DNA damage in Comet assay. Additionally, ROS induction in hemocytes and phenotypic alterations were determined in the mouths and legs of Drosophila. This study is the first study reporting genotoxicity data in the somatic cells of Drosophila larvae, emphasizing the importance of D. melanogaster as a model organism in investigating the different biological effects in a concentrationdependent manner caused by B 2 O 3 NPs and its ionic form. The obtained in vivo results contribute to improvement the genotoxicity database on the B 2 O 3 NPs.

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“…extract-treated vials every day. Mortality was recorded in each vial until the last fly died on every alternative day [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

Kushalan

D’Souza

Aloysius

et al. 2022

IJERPH

Self Cite

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Curculigo orchioides is used in Indian and Chinese traditional medicinal systems for various health benefits. However, its toxicological effects are mostly unknown. This study assesses the potential toxicity of aqueous leaf (A.L.) extract of C. orchioides using Drosophila melanogaster as an experimental model. Preliminary phytochemical tests were followed by the Fourier transform infrared (FTIR) tests to identify the functional group in the A.L. extract of C. orchioides. Drosophila larvae/adults were exposed to varying concentrations of C. orchioides A.L. extract through diet, and developmental, lifespan, reproduction, and locomotory behaviour assays were carried out to assess the C. orchioides toxicity at organismal levels. The cellular toxicity of A.L. extract was examined by analysing the expression of heat shock protein (hsps), reactive oxygen species (ROS) levels, and cell death. The FTIR analysis showed the presence of functional groups indicating the presence of secondary metabolites like saponins, phenolics, and alkaloids. Exposure to A.L. extract during development resulted in reduced emergence and wing malformations in the emerged fly. Furthermore, a significant reduction in reproductive performance and the organism’s lifespan was observed when adult flies were exposed to A.L. extract. This study indicates the adverse effect of C. orchioides A.L. extract on Drosophila and raises concerns about the practice of indiscriminate therapeutic use of plant extracts.

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Systematic toxicity assessment of CdTe quantum dots in Drosophila melanogaster

Cited by 23 publications

References 55 publications

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

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Systematic toxicity assessment of CdTe quantum dots in Drosophila melanogaster

Cited by 23 publications

References 55 publications

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

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Product

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In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use