Systematical characterization of YUCCA gene family in five cotton species, and potential functions of YUCCA22 gene in drought resistance of cotton

Wang, Xiaoyan; Chen, Baizhi; Ma, Changkai; Qiao, Kaikai; Li, Zhanshuai; Wang, Jingshun; Peng, Renhai; Fan, Shuli; Ma, Qin

doi:10.1016/j.indcrop.2021.113290

Cited by 8 publications

(10 citation statements)

References 59 publications

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“…The second true leaves of GhROP3-silenced and negative control plants before and after drought treatment for 10 days were selected. The relative water content (RWC) was measured by the oven drying method as described by Wang et al [42]. The contents of proline (Pro), hydrogen peroxide (H 2 O 2 ), and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in the leaves were determined according to the experimental steps mentioned in the instruction manual of the physiological index determination kit (Solarbio, Beijing, China).…”

Section: Drought Treatment and Determination Of Physiological And Bio...mentioning

confidence: 99%

“…Similarly, the ORF of GhGGB (MK105923) was amplified by PCR, digested, and inserted into a pGADT7 yeast expression vector to construct a pGADT7-GhGGB vector. The preparation, pGBKT7-GhROP3 self-activation detection, co-transformation, and culture of AH109 yeast competent cells were performed by previously described methods [42].…”

Section: Yeast Two-hybrid Assaymentioning

confidence: 99%

“…GhROP3 and GhGGB were cloned into the pCambia1300-cEYFP and pCambia1300-nEYFP vectors, respectively. The transformation of Agrobacterium tumefaciens EHA105, the co-injection of tobacco leaves, and fluorescence detection were performed by previously described methods [42].…”

Section: Bimolecular Fluorescence Complementation Experimentsmentioning

confidence: 99%

“…The evolutionary tree analysis results showed that GhROP3 belonged to class I ROP proteins and might be involved in prenylation modification reactions, which were speculated to have interactions with GhGGB proteins (Figure 1B). co-injection of tobacco leaves, and fluorescence detection were performed by previously described methods [42].…”

Section: Phylogenetic Analysis Of Ghrop3mentioning

confidence: 99%

“…The YUCCA (YUC) genes encode flavin monooxygenases involved in auxin biosynthesis [70]. GhYUC22 downregulation reduced the sensitivity of cotton to drought by reducing the content of IAA and increasing the content of ABA and the expression of related genes [42]. Auxin/Indoleacetic acid (Aux/IAA) proteins repress auxin-responsive transcription through inactivating ARF function [71].…”

Section: Ghrop3 Played a Negative Role In The Response Of Plants To D...mentioning

confidence: 99%

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A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.)

Lei

Dai

et al. 2022

Plants

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As a plant-specific Rho-like small G protein, the ROP (Rho-related GTPase of plants) protein regulates the growth and development of plants and various stress responses in the form of molecular switches. Drought is a major abiotic stress that limits cotton yield and fiber quality. In this study, virus-induced gene silencing (VIGS) technology was used to analyze the biological function of GhROP3 in cotton drought stress tolerance. Meanwhile, we used yeast two-hybrid and bimolecular fluorescence complementation assays to examine the interaction between GhROP3 and GhGGB. GhROP3 has a high expression level in cotton true leaves and roots, and responds to drought, high salt, cold, heat stress, and exogenous abscisic acid (ABA) and auxin (IAA) treatments. Silencing GhROP3 improved the drought tolerance of cotton. The water loss rates (WLR) of detached leaves significantly reduced in silenced plants. Also, the relative water content (RWC) and total contents of chlorophyll (Chl) and proline (Pro) of leaves after drought stress and the activities of three antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) significantly increased, whereas the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) significantly reduced. In the leaves of silenced plants, the expression of genes related to ABA synthesis and its related pathway was significantly upregulated, and the expression of decomposition-related GhCYP707A gene and genes related to IAA synthesis and its related pathways was significantly downregulated. It indicated that GhROP3 was a negative regulator of cotton response to drought by participating in the negative regulation of the ABA signaling pathway and the positive regulation of the IAA signaling pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhROP3 protein interacted with the GhGGB protein in vivo and in vitro. This study provided a theoretical basis for the in-depth investigation of the drought resistance–related molecular mechanism of the GhROP3 gene and the biological function of the GhGGB gene.

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Section: Drought Treatment and Determination Of Physiological And Bio...mentioning

confidence: 99%

Section: Yeast Two-hybrid Assaymentioning

confidence: 99%

Section: Bimolecular Fluorescence Complementation Experimentsmentioning

confidence: 99%

Section: Phylogenetic Analysis Of Ghrop3mentioning

confidence: 99%

Section: Ghrop3 Played a Negative Role In The Response Of Plants To D...mentioning

confidence: 99%

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A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.)

Lei

Dai

et al. 2022

Plants

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Defense Mechanism of Capsicum annuum L. Infected with Pepper Mild Mottle Virus Induced by Vanisulfane

Shi

et al. 2022

J. Agric. Food Chem.

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Pepper mild mottle virus (PMMoV), an RNA virus, is one of the most devastating pathogens in pepper crops and has a significant influence on global crop yields. PMMoV poses a major threat to the global shortage of pepper plants and other Solanaceae crops due to the lack of an effective antiviral agent. In this study, we have developed a plant immune inducer (vanisulfane), as a "plant vaccine" that boosts plant immunity against PMMoV, and studied its resistance mechanism. The protective activity of vanisulfane against PMMoV was 59.4%. Vanisulfane can enhance the activity of defense enzymes and improve the content of chlorophyll, flavonoids, and total phenols for removing harmful free radicals from plants. Furthermore, vanisulfane was found to enhance defense genes. Label-free quantitative proteomics would tackle disease resistance pathways of vanisulfane. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, differentially abundant proteins (DAPs) are mainly involved in starch and sucrose metabolism, photosynthesis, MAPK signaling pathway, and oxidative phosphorylation pathway. These results are crucial for the discovery of new pesticides, understanding the improvement of plant immunity and the antiviral activity of plant immune inducers.

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GhSTP18, a member of sugar transport proteins family, negatively regulates salt stress in cotton

Qiao

Wang

et al. 2023

Physiologia Plantarum

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The sugar transporter protein (STP) family has been shown to play important roles in plant growth, development, and stress response. However, it has not been studied in cotton compared to other major crops. In this study, we identified 90 STP genes from four cotton species, performed bioinformatic analysis, and focused on the role of GhSTP18 in salt stress. According to our results, cotton STP proteins were divided into four subgroups according to the phylogenetic tree. A synteny analysis suggested that whole‐genome duplication (WGD) and segmental duplication were key drivers in the expansion of the STP gene family. The transcriptomic data analysis showed that 29 GhSTP genes exhibited sink‐specific expression. Quantitative real time‐polymerase chain reaction (qRT‐PCR) analyses revealed that expression of GhSTP18 was induced by salt treatment, heat treatment, cold treatment, and drought treatment, and continuously increased during a salt stress time course. Notably, GhSTP18 encodes a plasma membrane‐localized galactose transporter. Suppression of GhSTP18 transcription by a virus‐induced gene silencing (VIGS) assay reduced sensitivity to salt stress in cotton, indicating that GhSTP18 negatively regulates plant salt tolerance. These results provide an important reference and resource for further studying and deploying STP genes for cotton improvement.

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Systematical characterization of YUCCA gene family in five cotton species, and potential functions of YUCCA22 gene in drought resistance of cotton

Cited by 8 publications

References 59 publications

A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.)

A Small Gtp-Binding Protein GhROP3 Interacts with GhGGB Protein and Negatively Regulates Drought Tolerance in Cotton (Gossypium hirsutum L.)

Defense Mechanism of Capsicum annuum L. Infected with Pepper Mild Mottle Virus Induced by Vanisulfane

GhSTP18, a member of sugar transport proteins family, negatively regulates salt stress in cotton

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