T-Lymphocyte Profile and Total and Virus-Specific Immunoglobulin Concentrations in the Cervix of HIV-1-Infected Women

Quayle, Alison J.; Kourtis, Athena P.; Cu‐Uvin, Susan; Politch, Joseph A.; Yang, Huixia; Bowman, Frederick P.; Shah, Meha; Anderson, Deborah J.; Crowley-Nowick, Peggy A.; Duerr, Ann

doi:10.1097/qai.0b013e31802c5b3a

Cited by 20 publications

(16 citation statements)

References 24 publications

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“…We also observed no difference in CD4 + memory T cell α4β7 expression between the FRT and CVS of BLT mice (P = 0.13) (Supplemental Figure 3). In summary, the majority of lymphocytes in the FRT and CVS of BLT mice were memory T cells expressing CCR5, which is remarkably similar to what is observed in the FRT of humans (32,33,37).…”

Section: Reconstitution Of the Frt Of Blt Mice With Human Cd4supporting

confidence: 62%

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

Olesen

Swanson

Kovářová

et al. 2016

Journal of Clinical Investigation

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Section: Reconstitution Of the Frt Of Blt Mice With Human Cd4supporting

confidence: 62%

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

Olesen

Swanson

Kovářová

et al. 2016

Journal of Clinical Investigation

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“…In the present study, we observed that percentages of DR+38+CD4+ T-cells were six-times higher in the cervix than in the blood of healthy HIV-1 seronegative women, consistent with two other small studies. 22–23 Reasons for higher proportions of activated CD4+ T-cells in the cervix are unknown, although, higher percentages of memory T-cell populations are found in the cervix than blood. 24–25 It has been proposed that elevated immune activation in the cervix is a result of antigenic stimulation.…”

Section: Discussionmentioning

confidence: 99%

CCR5 Expression Is Elevated on Endocervical CD4+ T Cells in Healthy Postmenopausal Women

Meditz

Moreau

MaWhinney

et al. 2012

JAIDS Journal of Acquired Immune Deficiency Syndromes

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Background New HIV-1 infections are increasing in older American women largely through heterosexual transmission. Activated CD4+ T-cells and CCR5 expression are linked to HIV-1 susceptibility, but whether these parameters are altered in the cervix of older women is unknown. Methods Whole blood and in some instances endocervical brush samples were collected from healthy premenopausal (n=22) and postmenopausal women (n=24). Percentages of HLA-DR(DR)+CD38(38)+CD4+ T-cells, and HIV-1 chemokine coreceptor expression were determined by flow cytometry. Results Percentages of DR+38+CD4+ T-cells were 6-times greater in cervix (median, 6.4%) than blood (median, 1.1%; p<0.001), but did not differ within each compartment between premenopausal and postmenopausal women (p=0.2). Postmenopausal women had greater percentages of CCR5+CD4+ and CCR5+DR+38+CD4+ T-cells compared to premenopausal women in cervix (median, 70% vs. 42%, p=0.005; and 80% vs. 57%; p<0.05, respectively) and blood (medians, 22% vs. 13%, and 76% vs. 62%, respectively; p<0.001). Postmenopausal women had more CCR5 molecules on cervical DR+38+CD4+ T-cells (median, 3,176) than premenopausal women (median, 1,776; p=0.02). Age and percent CCR5+CD4+ and CCR5+DR+38+CD4+-cells were linearly related in cervix (r2=0.47, p<0.001 and r2=0.25, p=0.01, respectively) and blood (r2=0.20, p=0.001 and r2=0.31, respectively; p<0.001), but confounding of age with menopause could not be excluded. Cervical CXCR4 expression did not differ substantially between premenopausal and postmenopausal women. Conclusions Elevated cervical CCR5 expression in postmenopausal women may increase their risk for HIV-1 acquisition. Studies are needed to confirm whether elevated CCR5 expression confers increased HIV-1 susceptibility in postmenopausal women, and if it is related to hormonal or nonhormonal effects of aging.

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“…Endocervical secretion samples were obtained by a 360-degree rotation in the os, and vaginal secretion samples were collected by rotating the swab along the vaginal vault. Afterward, a cytobrush (Cooper Surgical) was inserted in the endocervical os to collect cervical cells, placed in Roswell Park Memorial Institute 1640 medium supplemented with gentamycin and amphotericin B, and processed within 4 h of collection [25,37]. Swab samples collected for genital HIV level analyses were stored in 1 mL of RNAlater solution at 4ЊC until processed for storage at Ϫ80ЊC, as described elsewhere [35].…”

Section: Population and Clinical Data Eleven Women Attending A Newmentioning

confidence: 99%

Elevated Cervical White Blood Cell Infiltrate Is Associated with Genital HIV Detection in a Longitudinal Cohort of Antiretroviral Therapy–Adherent Women

et al. 2010

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T-Lymphocyte Profile and Total and Virus-Specific Immunoglobulin Concentrations in the Cervix of HIV-1-Infected Women

Abstract: HIV infection induces substantial changes in the immune profile of the female genital tract. Further study of the implications of these findings for HIV acquisition and transmission is needed.

Cited by 20 publications

References 24 publications

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

CCR5 Expression Is Elevated on Endocervical CD4+ T Cells in Healthy Postmenopausal Women

Elevated Cervical White Blood Cell Infiltrate Is Associated with Genital HIV Detection in a Longitudinal Cohort of Antiretroviral Therapy–Adherent Women

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