T-RFLP combined with principal component analysis and 16S rRNA gene sequencing: an effective strategy for comparison of fecal microbiota in infants of different ages

Wang, Mei; Ahrné, Siv; Antonsson, Martin; Molin, Göran

doi:10.1016/j.mimet.2004.06.002

Cited by 103 publications

(81 citation statements)

References 38 publications

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“…bifidobacteria, clostridia and Bacteroides spp. regardless of the infant's geographical origin and methods used for the detection (65,(75)(76)(77)(78)(79)(80) . Immediately after birth, the rectal microbiota of vaginally delivered babies resembles their own mother's vaginal microbiota, whereas the rectal microbiota of babies delivered by Caesarean section resembles that of the skin (81) .…”

Section: Agementioning

confidence: 99%

Human gut microbiota: does diet matter?

2014

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The human oro-gastrointestinal (GI) tract is a complex system, consisting of oral cavity, pharynx, oesophagus, stomach, small intestine, large intestine, rectum and anus, which all together with the accessory digestive organs constitute the digestive system. The function of the digestive system is to break down dietary constituents into small molecules and then absorb these for subsequent distribution throughout the body. Besides digestion and carbohydrate metabolism, the indigenous microbiota has an important influence on host physiological, nutritional and immunological processes, and commensal bacteria are able to modulate the expression of host genes that regulate diverse and fundamental physiological functions. The main external factors that can affect the composition of the microbial community in generally healthy adults include major dietary changes and antibiotic therapy. Changes in some selected bacterial groups have been observed due to controlled changes to the normal diet e.g. high-protein diet, high-fat diet, prebiotics, probiotics and polyphenols. More specifically, changes in the type and quantity of non-digestible carbohydrates in the human diet influence both the metabolic products formed in the lower regions of the GI tract and the bacterial populations detected in faeces. The interactions between dietary factors, gut microbiota and host metabolism are increasingly demonstrated to be important for maintaining homeostasis and health. Therefore the aim of this review is to summarise the effect of diet, and especially dietary interventions, on the human gut microbiota. Furthermore, the most important confounding factors (methodologies used and intrinsic human factors) in relation to gut microbiota analyses are elucidated.

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Section: Agementioning

confidence: 99%

Human gut microbiota: does diet matter?

2014

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“…Microarray experiments indicated that the overall viral community composition changed between 1 and 2 weeks of age, a phenomenon also observed for the infant intestinal microbiota. 41,42 A metagenomic study of the RNA-containing viral community in human feces of healthy individuals indicated the prevalence of plant pathogenic RNA viruses, presumably acquired via dietary sources. 43 Letarov and Kulikov 12 suggested that ingestion of these particles with food occurs at a much higher rate than the internal production of RNA-containing phages.…”

Section: Bacteriophage Abundance and Diversity In The Human Intestinementioning

confidence: 99%

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et al. 2013

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“…4 The microbiota acquired in early life have longterm implications for host metabolism and gastrointestinal (GI), immune and neurological function. 5,6 Reduced diversity or dysbiosis are linked to childhood and later life disorders, including necrotizing enterocolitis, 7 eczema, 8 asthma, 9 inflammatory bowel diseases, 10 irritable bowel syndrome, 11 obesity, 12 diabetes 13 and autism. 14 Diet is one of the major determinants of GI microbial diversity.…”

Section: Introductionmentioning

confidence: 99%

The role of early life nutrition in the establishment of gastrointestinal microbial composition and function

2017

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The development of the human infant intestinal microbiota is a sequential process that begins in utero and continues during the first 2 to 3 years of life. Microbial composition and diversity are shaped by host genetics and multiple environmental factors, of which diet is a principal contributor. An understanding of this process is of clinical importance as the microbiota acquired in early life influence gastrointestinal, immune and neural development, and reduced microbial diversity or dysbiosis during infancy is associated with disorders in infancy and later childhood. The goal of this article was to review the published literature that used culture-independent methods to describe the development of the gastrointestinal microbiota in breast-and formula-fed human infants as well as the impact of prebiotic and probiotic addition to infant formula, and the addition of solid foods.

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T-RFLP combined with principal component analysis and 16S rRNA gene sequencing: an effective strategy for comparison of fecal microbiota in infants of different ages

Cited by 103 publications

References 38 publications

Human gut microbiota: does diet matter?

Human gut microbiota: does diet matter?

Movers and shakers

The role of early life nutrition in the establishment of gastrointestinal microbial composition and function

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