Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria

Kumari, Madhuree; Pandey, Shipra; Giri, Ved Prakash; Bhattacharya, Arpita; Shukla, Richa; Mishra, Aradhana; Nautiyal, Chandra Shekhar

doi:10.1016/j.micpath.2016.11.012

Cited by 87 publications

(61 citation statements)

References 44 publications

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“…In a study with Aspergillus oryzae, silver nanoparticles were also synthesized in the dark forming peak at 420 nm and FTIR spectrum showed presence of similar functional groups with our study, i.e., N-H vibrations and C-H stretch. Some additional functional groups were also present like C=O, C=C, C-C, and C=N [34,35].…”

Section: Discussionmentioning

confidence: 99%

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Shahzad

Saeed

Iqtedar

et al. 2019

Journal of Nanomaterials

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The biogenesis of silver nanoparticles by fungi is an ecologically clean and nontoxic method compared to other physical and chemical methods. Thus, we aimed to discuss the mycosynthesis of extracellular size-controlled AgNPs. After comprehensive screening, Aspergillus fumigatus BTCB10 (KY486782) was selected for the synthesis of AgNPs of controlled size. Characterization was performed by UV-Vis spectrophotometer, Zetasizer, X-Ray Diffraction (XRD), FTIR (Fourier-transform infrared), Atomic Force Microscopy (AFM), and Scanning Electron Microscopy (SEM) along with functional assays—antibacterial and MTT assays. Data suggested that under optimized conditions, i.e., temperature 25°C, AgNO3 concentration 1 mM, biomass 7 g, fungal culture age 7 days, pH 6, ratio of cell-free filtrate (CFF)/silver nitrate (3 : 2), NaCl 20%, and under dark light, the smallest size AgNPs of 0.681 nm with 100% monodispersity was obtained as evident by a zeta potential of -23.4 mV, UV-Vis band at 400 nm, and the presence of O-H and C=O groups confirmed by ATR-FTIR; XRD revealed the crystalline nature of AgNPs; additionally, cube-shaped AgNPs were revealed by Scanning Electron Microscopy (SEM). Moreover, synthesized AgNPs exhibited antibacterial activity against multidrug-resistant bacterial strains, notably, Klebsiella pneumoniae BTCB04, Acinetobacter BTCB05, Pseudomonas aeruginosa BTCB01, and Escherichia coli BTCB03, while maximum 7-fold was observed with Acinetobacter BTCB05. AgNPs demonstrated no cytotoxic activity against HepG2 cells; however, in combination with cisplatin, antiproliferative and cytotoxic effects became more evident and significant in comparison to control and as single agent. Taken together, the data suggested that economical and smallest size AgNPs can be biosynthesized from Aspergillus fumigatus BTCB10 and be used as antibacterial and antiproliferative agents in combination with current drugs against clinically relevant multiple drug-resistant bacterial and tumoral cells. Further studies are required to confirm their effects employing in vivo disease models.

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Section: Discussionmentioning

confidence: 99%

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Shahzad

Saeed

Iqtedar

et al. 2019

Journal of Nanomaterials

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“…87 In another study, spherical, rectangular, penta, and hexagonal AgNPs of different dimensions were biosynthesized, and the results showed that the spherical AgNPs possessed more antimicrobial effect than other shaped AgNPs. 88 The electron charge transfer properties are also affected by different types of AgNPs. Regardless of the size required, narrow size distributions and more "spherical" shapes can improve sample quality.…”

Section: Adjustment Of Physical Propertiesmentioning

confidence: 99%

Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies

Qing

Cheng

et al. 2018

IJN

761

362

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Infection, as a common postoperative complication of orthopedic surgery, is the main reason leading to implant failure. Silver nanoparticles (AgNPs) are considered as a promising antibacterial agent and always used to modify orthopedic implants to prevent infection. To optimize the implants in a reasonable manner, it is critical for us to know the specific antibacterial mechanism, which is still unclear. In this review, we analyzed the potential antibacterial mechanisms of AgNPs, and the influences of AgNPs on osteogenic-related cells, including cellular adhesion, proliferation, and differentiation, were also discussed. In addition, methods to enhance biocompatibility of AgNPs as well as advanced implants modifications technologies were also summarized.

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“…In our study, the more spherical-like particles such as the intracellular runs 1 (pH 9, 1 mM), 3 (pH 9, 3 mM), and 7 (pH 6, 3 mM) displayed a high antimicrobial effect, in accordance with several studies. [50][51][52] While the size of the NP is one of the most influential aspects in terms of antimicrobial activity, it is not the only one: The amount of Ag that the NP contains can also influence the antimicrobial activity, as can the shape of the particle. Some studies have reported an increased antimicrobial activity in NPs with triangular shapes, due to the fact that the sharp corners of the NP can penetrate the cellular membrane more easily.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

<em>Botryococcus braunii</em> as a bioreactor for the production of nanoparticles with antimicrobial potentialities

Arévalo-Gallegos¹,

García‐Pérez²,

Carrillo-Nieves³

et al. 2018

IJN

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BackgroundMicroalgae produce metabolites with notable potentialities to act as reducing and capping agents for the synthesis of silver nanoparticles (AgNPs) in a process widely recognized as an eco-friendly and cheaper alternative for the generation of nanoparticles (NPs).MethodsIn the present work, AgNPs were synthesized using live Botryococcus braunii cultures. Two biosynthesis routes were explored: (1) intracellular and (2) extracellular at pH levels of 6–9 using 1–5 mM silver nitrate concentrations.ResultsThe generation of NPs was confirmed via ultraviolet–visible spectroscopy. The morphological characteristics were observed using scanning electron microscopy which revealed that the newly developed AgNPs were mostly spherical in sizes starting from 168 nm. The characteristic peaks in a typical Fourier transform infrared spectroscopy suggested that the exopolysaccharides were the possible reducing and capping agents. The antimicrobial spectrum of the newly developed AgNPs was tested against bacterial strains, both Gram-negative, Gram-positive, and yeast, ie, Escherichia coli (American Type Culture Collection [ATCC] 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and the yeast Candida albicans (ATCC 10231), respectively. The antimicrobial activity tests showed a stronger inhibition against Gram-negative bacteria. Statistically, the NPs biosynthesized at pH values of 6 and 8 displayed a higher antimicrobial activity.ConclusionOur findings showed that B. braunii is capable of generating AgNPs with antimicrobial potential.

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Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria

Cited by 87 publications

References 44 publications

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies

<em>Botryococcus braunii</em> as a bioreactor for the production of nanoparticles with antimicrobial potentialities

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