Targeted Acquisition of Fusarium oxysporum f. sp. niveum Toxin-Deficient Mutant and Its Effects on Watermelon Fusarium Wilt

Xie, Xing‐Guang; Huang, Chun-Yan; Cai, Zhendong; Chen, Yan; Dai, Chuan‐Chao

doi:10.1021/acs.jafc.9b02172

Cited by 12 publications

(6 citation statements)

References 44 publications

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“…cucumerium and F. oxysporum f. sp. niveum, 4,5 and stem blight of asparagus caused by Phomopsis asparagi (P. aragi). 6 At the same time, some marine phytoplankton species (microalgae), such as Amphidinium carterae (A. carterae), Heterocapsa circularisquama (H. circularisquama), Heterosigma akashiwo (H. akashiwo), and Prorocentrum donghaiense (P. donghaiense), can often cause red tides that do harm to the development of mariculture.…”

Section: ■ Introductionmentioning

confidence: 99%

Antifungal and Antimicroalgal Trichothecene Sesquiterpenes from the Marine Algicolous Fungus Trichoderma brevicompactum A-DL-9-2

Shi

Liu

et al. 2020

J. Agric. Food Chem.

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Eight new trichothecene derivatives, trichodermarins G-N (1−8), and two new cuparene derivatives, trichocuparins A (9) and B (10), as well as six known trichothecenes (11−16) were isolated from the fungal strain Trichoderma brevicompactum A-DL-9-2 obtained from the inner tissue of the marine red alga Chondria tenuissima. The structures and relative configurations of 1−10 were assigned by NMR and MS data, and the absolute configurations of 1, 2, and 9 were established by X-ray diffraction. Compound 8 features an aminosugar unit bond to the trichothecene framework for the first time, while 9 and 10 represent the first occurrence of cuparene sesquiterpenes in Trichoderma. All the isolates were assayed for growth inhibition of five phytopathogenic fungi (Botrytis cinerea, Cochliobolus miyabeanus, Fusarium oxysporum f. sp. cucumerium, Fusarium oxysporum f. sp. niveum, and Phomopsis asparagi) and four marine phytoplankton species (Amphidinium carterae, Heterocapsa circularisquama, Heterosigma akashiwo, and Prorocentrum donghaiense). Several of them exhibited significant inhibitory activities against the fungi and phytoplankton tested of which trichodermin (12) showed the highest antifungal and antimicroalgal activities with MIC and IC 50 values being 4.0 and 0.82 μg/mL, respectively.

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Section: ■ Introductionmentioning

confidence: 99%

Antifungal and Antimicroalgal Trichothecene Sesquiterpenes from the Marine Algicolous Fungus Trichoderma brevicompactum A-DL-9-2

Shi

Liu

et al. 2020

J. Agric. Food Chem.

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“…Another approach is using non-pathogenic F. oxysporum, which antagonizes the pathogenic Fo or primes host immune response. Therefore, understanding the pathogenesis and virulence of Fo is pivotal in controlling Fusarium wilt in the field [6].…”

Section: Introductionmentioning

confidence: 99%

Degenerated Virulence and Irregular Development of Fusarium oxysporum f. sp. niveum Induced by Successive Subculture

Chang

Lin

Wan

et al. 2020

JoF

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Successive cultivation of fungi on artificial media has been reported to cause the sectorization, which leads to degeneration of developmental phenotype, and virulence. Fusarium oxysporum f. sp. niveum (Fon), the causal agent of watermelon Fusarium wilt, forms degenerated sectors after successive cultivation. In the present research, we demonstrated that subculture with aged mycelia increased the incidence of degenerations. To further investigate the differences between the Fon wild type (sporodochial type, ST) and variants (MT: mycelial type and PT: pionnotal type), developmental phenotypes and pathogenicity to watermelon were examined. Results in variants (PT2, PT3, PT11, and MT6) were different from ST with mycelia growth, conidia production and chlamydospore formation. Virulence of degenerated variants on susceptible watermelon Grand Baby (GB) cultivar was determined after inoculation with Fon variants and Fon ST. In root dipping methods, Fon variants showed no significant differences in disease progress compared with ST. Fon variants showed a significant decrease in disease progression compared with ST through infested soil inoculation. The contrasting results of two inoculation methods suggest that the degenerative changes due to repeated successive cultivation may lead to the loss of pathogen virulence-related factors of the early stage of Fon infection process. Therefore, cell wall-degrading enzymes (CWDEs; cellulase, pectinase, and xylanase) activities of different variants were analyzed. All Fon degenerated variants demonstrated significant decreases of CWDEs activities compared with ST. Additionally, transcript levels of 9 virulence-related genes (fmk1, fgb1, pacC, xlnR, pl1, rho1, gas1, wc1, and fow1) were assessed in normal state. The degenerated variants demonstrated a significantly low level of tested virulence-related gene transcripts except for fmk1, xlnR, and fow1. In summary, the degeneration of Fon is triggered with successive subculture through aged mycelia. The degeneration showed significant impacts on virulence to watermelon, which was correlated with the reduction of CWDEs activities and declining expression of a set of virulence-related genes.

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“…Mycotoxins from fungi are key factors on the process of establishment of infection in plants, and fusaric acid from F. oxysporum may interfere on metabolic pathways of host plant during invasion process [35]. Recently, a study with fusaric acid-deficient mutant was evaluated and showed to increase the resistance in watermelon by Fusarium proliferation [39].…”

Section: Resultsmentioning

confidence: 99%

Mycotoxins from Fusarium proliferatum: new inhibitors of papain-like cysteine proteases

et al. 2020

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Papain-like cysteine proteases (PLCPs) in plants are essential to prevent phytopathogen invasion. In order to search for cysteine protease inhibitors and to investigate compounds that could be associated to pineapple Fusarium disease, a chemistry investigation was performed on Fusarium proliferatum isolated from Ananas comosus (pineapple) and cultivated in Czapek medium. From F. proliferatum extracts, nine secondary metabolites were isolated and characterized by nuclear magnetic resonance spectroscopy and mass spectrometry experiments: beauvericin (1), fusaric acid (2), N-ethyl-3-phenylacetamide (3), Nacetyltryptamine (4), cyclo(L-Val-L-Pro) cyclodipeptide (5), cyclo(L-Leu-L-Pro) cyclodipeptide (6), cyclo(L-Leu-L-Pro) diketopiperazine (7), 2,4-dihydroxypyrimidine (8), and 1H-indole-3-carbaldehyde (9). Compounds 1, 3, and 6 showed significant inhibition of papain, with IC 50 values of 25.3 ± 1.9, 39.4 ± 2.5, and 7.4 ± 0.5 μM, respectively. Compound 1 also showed significant inhibition against human cathepsins V and B with IC 50 of 46.0 ± 3.0 and 6.8 ± 0.7 μM, respectively. The inhibition of papain by mycotoxins (fusaric acid and beauvericin) may indicate a mechanism of Fusarium in the roles of infection process.

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Targeted Acquisition of Fusarium oxysporum f. sp. niveum Toxin-Deficient Mutant and Its Effects on Watermelon Fusarium Wilt

Cited by 12 publications

References 44 publications

Antifungal and Antimicroalgal Trichothecene Sesquiterpenes from the Marine Algicolous Fungus Trichoderma brevicompactum A-DL-9-2

Antifungal and Antimicroalgal Trichothecene Sesquiterpenes from the Marine Algicolous Fungus Trichoderma brevicompactum A-DL-9-2

Degenerated Virulence and Irregular Development of Fusarium oxysporum f. sp. niveum Induced by Successive Subculture

Mycotoxins from Fusarium proliferatum: new inhibitors of papain-like cysteine proteases

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