Targeted Metabolomics of Plant Hormones and Redox Metabolites in Stomatal Immunity

David, Lisa; Kang, Jianing; Chen, Sixue

doi:10.1007/978-1-0716-0142-6_6

Cited by 23 publications

(13 citation statements)

References 20 publications

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“…However, it should be noted that the difference between the control group and the salt-treatment group was similar to some of the non-CAM halophytes (Ksouri et al, 2007;Amor et al, 2005), or significantly smaller than some non-halophytes (Liang, 1999;Sreenivasulu et al, 1999). Since reactive oxygen species and oxidative stress affect cellular redox state, and many biological processes including photosynthesis are regulated by redox, future studies focusing on redox regulation [e.g., using redox proteomics and metabolomics (David et al, 2019;Yu et al, 2020)] can be expected to reveal molecular mechanisms underlying the C 3 to CAM transition in M. crystallinum plants.…”

Section: Discussionmentioning

confidence: 97%

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

et al. 2020

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Salt stress impedes plant growth and development, and leads to yield loss. Recently, a halophyte species Mesembryanthemum crystallinum has become a model to study plant photosynthetic responses to salt stress. It has an adaptive mechanism of shifting from C 3 photosynthesis to crassulacean acid metabolism (CAM) photosynthesis under stresses, which greatly enhances water usage efficiency and stress tolerance. In this study, we focused on investigating the morphological and physiological changes [e.g., leaf area, stomatal movement behavior, gas exchange, leaf succulence, and relative water content (RWC)] of M. crystallinum during the C 3 to CAM photosynthetic transition under salt stress. Our results showed that in M. crystallinum seedlings, CAM photosynthesis was initiated after 6 days of salt treatment, the transition takes place within a 3-day period, and plants became mostly CAM in 2 weeks. This result defined the transition period of a facultative CAM plant, laid a foundation for future studies on identifying the molecular switches responsible for the transition from C 3 to CAM, and contributed to the ultimate goal of engineering CAM characteristics into C 3 crops.

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Section: Discussionmentioning

confidence: 97%

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

et al. 2020

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“…This result is consistent with a recent report [ 57 ] where decreased transpiration rates in primed Arabidopsis leaves were observed and related to decreases in photosynthesis-related transcripts in the primed leaves. Previously, we have identified increases in SA in primed guard cells compared to mock guard cells using targeted metabolomics [ 64 ]. Here we were able to reproduce this result using untargeted metabolomics.…”

Section: Discussionmentioning

confidence: 99%

“…Enriched guard cell samples were prepared as previously described [ 64 ]. Briefly, for each sample, 144 upper uninfected leaves were collected from 36 individual plants (i.e., four upper primed leaves per plant).…”

Section: Methodsmentioning

confidence: 99%

Multi-Omics Revealed Molecular Mechanisms Underlying Guard Cell Systemic Acquired Resistance

David

Kang

Dufresne

et al. 2020

IJMS

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Systemic Acquired Resistance (SAR) improves immunity of plant systemic tissue after local exposure to a pathogen. Guard cells that form stomatal pores on leaf surfaces recognize bacterial pathogens via pattern recognition receptors, such as Flagellin Sensitive 2 (FLS2). However, how SAR affects stomatal immunity is not known. In this study, we aim to reveal molecular mechanisms underlying the guard cell response to SAR using multi-omics of proteins, metabolites and lipids. Arabidopsis plants previously exposed to pathogenic bacteria Pseudomonas syringae pv. tomato DC3000 (Pst) exhibit an altered stomatal response compared to control plants when they are later exposed to the bacteria. Reduced stomatal apertures of SAR primed plants lead to decreased number of bacteria in leaves. Multi-omics has revealed molecular components of SAR response specific to guard cells functions, including potential roles of reactive oxygen species (ROS) and fatty acid signaling. Our results show an increase in palmitic acid and its derivative in the primed guard cells. Palmitic acid may play a role as an activator of FLS2, which initiates stomatal immune response. Improved understanding of how SAR signals affect stomatal immunity can aid biotechnology and marker-based breeding of crops for enhanced disease resistance.

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“…40 pmol g −1 JA and approx. 0.83 nmol g −1 SA) in guard‐cell‐enriched epidermal peels collected from Arabidopsis plants (David et al., 2020). The quantification of JA and SA in Arabidopsis leaves revealed similar ranges of the hormones (Forcat et al., 2008; Pan et al., 2010; Trapp et al., 2014).…”

Section: Resultsmentioning

confidence: 99%

Jasmonic acid and salicylic acid play minor roles in stomatal regulation by CO₂, abscisic acid, darkness, vapor pressure deficit and ozone

et al. 2021

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SUMMARY Jasmonic acid (JA) and salicylic acid (SA) regulate stomatal closure, preventing pathogen invasion into plants. However, to what extent abscisic acid (ABA), SA and JA interact, and what the roles of SA and JA are in stomatal responses to environmental cues, remains unclear. Here, by using intact plant gas‐exchange measurements in JA and SA single and double mutants, we show that stomatal responsiveness to CO2, light intensity, ABA, high vapor pressure deficit and ozone either did not or, for some stimuli only, very slightly depended upon JA and SA biosynthesis and signaling mutants, including dde2, sid2, coi1, jai1, myc2 and npr1 alleles. Although the stomata in the mutants studied clearly responded to ABA, CO2, light and ozone, ABA‐triggered stomatal closure in npr1‐1 was slightly accelerated compared with the wild type. Stomatal reopening after ozone pulses was quicker in the coi1‐16 mutant than in the wild type. In intact Arabidopsis plants, spraying with methyl‐JA led to only a modest reduction in stomatal conductance 80 min after treatment, whereas ABA and CO2 induced pronounced stomatal closure within minutes. We could not document a reduction of stomatal conductance after spraying with SA. Coronatine‐induced stomatal opening was initiated slowly after 1.5–2.0 h, and reached a maximum by 3 h after spraying intact plants. Our results suggest that ABA, CO2 and light are major regulators of rapid guard cell signaling, whereas JA and SA could play only minor roles in the whole‐plant stomatal response to environmental cues in Arabidopsis and Solanum lycopersicum (tomato).

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Targeted Metabolomics of Plant Hormones and Redox Metabolites in Stomatal Immunity

Cited by 23 publications

References 20 publications

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

Multi-Omics Revealed Molecular Mechanisms Underlying Guard Cell Systemic Acquired Resistance

Jasmonic acid and salicylic acid play minor roles in stomatal regulation by CO₂, abscisic acid, darkness, vapor pressure deficit and ozone

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Targeted Metabolomics of Plant Hormones and Redox Metabolites in Stomatal Immunity

Cited by 23 publications

References 20 publications

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

Multi-Omics Revealed Molecular Mechanisms Underlying Guard Cell Systemic Acquired Resistance

Jasmonic acid and salicylic acid play minor roles in stomatal regulation by CO2, abscisic acid, darkness, vapor pressure deficit and ozone

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Jasmonic acid and salicylic acid play minor roles in stomatal regulation by CO₂, abscisic acid, darkness, vapor pressure deficit and ozone