Targeted mutagenesis and genetic analysis of a Drosophila receptor-linked protein tyrosine phosphatase gene

Hamilton, Bruce A.; Ho, A.; Zinn, Kai

doi:10.1007/bf00241271

Cited by 19 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ptp99A null larvae (genotype Ptp99A 1 /Df(3R)R3) (Hamilton et al, 1995) did not display MB phenotypes, and the overall structure of the brain was also normal (data not shown). Two of 28 adult MBs examined, however, had branching defects in which the α lobe was missing and the β lobe was enlarged (Fig.…”

Section: Ptp10d Mutants Display Core Fiber Segregation Phenotypesmentioning

confidence: 94%

See 1 more Smart Citation

Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body

Kurusu

Zinn

2008

Molecular and Cellular Neuroscience

Self Cite

View full text Add to dashboard Cite

Receptor tyrosine phosphatases (RPTPs) are required for axon guidance during embryonic development in Drosophila. Here we examine the roles of four RPTPs during development of the larval mushroom body (MB). MB neurons extend axons into parallel tracts known as the peduncle and lobes. The temporal order of neuronal birth is reflected in the organization of axons within these tracts. Axons of the youngest neurons, known as core fibers, extend within a single bundle at the center, while those of older neurons fill the outer layers. RPTPs are selectively expressed on the core fibers of the MB. Ptp10D and Ptp69D regulate segregation of the young axons into a single core bundle. Ptp69D signaling is required for axonal extension beyond the peduncle. Lar and Ptp69D are necessary for the axonal branching decisions that create the lobes. Avoidance of the brain midline by extending medial lobe axons involves signaling through Lar.

show abstract

Section: Ptp10d Mutants Display Core Fiber Segregation Phenotypesmentioning

confidence: 94%

“…Ptp69D RNAi strains (VDRC#4789 and #27091) were obtained from Vienna Drosophila RNAi Center. Ptp99A 1 and Df(3R)R3 are described in (Hamilton et al, 1995). Lar 5.1 , Lar 5.5 , Lar 13.2 , and Lar OD5 are described in (Krueger et al, 1996).…”

Section: Drosophila Stocksmentioning

confidence: 99%

Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body

Kurusu

Zinn

2008

Molecular and Cellular Neuroscience

Self Cite

View full text Add to dashboard Cite

show abstract

“…Only 5-10 kb of DNA are deleted in Ptp99A 1 /Df(3R)R3 flies, all of which is within the Ptp99A gene (Hamilton et al, 1995). Df(3R)KE/Df(3R)R3 flies lack essentially all DPTP99A coding sequences.…”

Section: Resultsmentioning

confidence: 99%

“…The mutant lines used in this study were described previously (Desai et al, 1996;Hamilton et al, 1995;Krueger et al, 1996). The genes previously called dptp69D and dptp99A have been renamed Ptp69D and Ptp99A, respectively, to conform to current Flybase nomenclature.…”

Section: Methodsmentioning

confidence: 99%

Competition and cooperation among receptor tyrosine phosphatases control motoneuron growth cone guidance in Drosophila

et al. 1997

View full text Add to dashboard Cite

The neural receptor tyrosine phosphatases DPTP69D, DPTP99A and DLAR are involved in motor axon guidance in the Drosophila embryo. Here we analyze the requirements for these three phosphatases in growth cone guidance decisions along the ISN and SNb motor pathways. Any one of the three suffices for the progression of ISN pioneer growth cones beyond their first intermediate target in the dorsal muscle field. DLAR or DPTP69D can facilitate outgrowth beyond a second intermediate target, and DLAR is uniquely required for formation of a normal terminal arbor. A different pattern of partial redundancy among the three phosphatases is observed for the SNb pathway. Any one of the three suffices to allow SNb axons to leave the common ISN pathway at the exit junction. When DLAR is not expressed, however, SNb axons sometimes bypass their ventrolateral muscle targets after leaving the common pathway, instead growing out as a separate bundle adjacent to the ISN. This abnormal guidance decision can be completely suppressed by also removing DPTP99A, suggesting that DLAR turns off or counteracts a DPTP99A signal that favors the bypass axon trajectory. Our results show that the relationships among the tyrosine phosphatases are complex and dependent on cellular context. At growth cone choice points along one nerve, two phosphatases cooperate, while along another nerve these same phosphatases can act in opposition to one another.

show abstract

“…To examine whether DPTP10D has a function in CNS axon guidance that is compensated for by another RPTP, we made double mutant combinations in which Ptp10D mutations were combined with mutations in each of the other three neural Rptp genes. Double mutants lacking DPTP10D and DPTP99A (Hamilton et al, 1995) are viable and exhibit no detectable embryonic phenotypes. Dlar mutations are lethal and confer motor axon guidance phenotypes (Krueger et al, 1996;Desai et al, 1997a), but these are unchanged when DPTP10D is also absent.…”

Section: Ptp10d Ptp69d Double Mutant Embryos Display a Synergistic Midline Crossing Phenotypementioning

confidence: 99%

Receptor tyrosine phosphatases regulate axon guidance across the midline of the Drosophila embryo

et al. 2000

View full text Add to dashboard Cite

Neural receptor-linked protein tyrosine phosphatases (RPTPs) are required for guidance of motoneuron and photoreceptor growth cones in Drosophila. These phosphatases have not been implicated in growth cone responses to specific guidance cues, however, so it is unknown which aspects of axonal pathfinding are controlled by their activities. Three RPTPs, known as DLAR, DPTP69D, and DPTP99A, have been genetically characterized thus far. Here we report the isolation of mutations in the fourth neural RPTP, DPTP10D. The analysis of double mutant phenotypes shows that DPTP10D and DPTP69D are necessary for repulsion of growth cones from the midline of the embryonic central nervous system. Repulsion is thought to be triggered by binding of the secreted protein Slit, which is expressed by midline glia, to Roundabout (Robo) receptors on growth cones. Robo repulsion is downregulated by the Commissureless (Comm) protein, allowing axons to cross the midline. Here we show that the Rptp mutations genetically interact with robo, slit and comm. The nature of these interactions suggests that DPTP10D and DPTP69D are positive regulators of Slit/Roundabout repulsive signaling. We also show that elimination of all four neural RPTPs converts most noncrossing longitudinal pathways into commissures that cross the midline, indicating that tyrosine phosphorylation controls the manner in which growth cones respond to midline signals.

show abstract

Targeted mutagenesis and genetic analysis of a Drosophila receptor-linked protein tyrosine phosphatase gene

Cited by 19 publications

References 26 publications

Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body

Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body

Competition and cooperation among receptor tyrosine phosphatases control motoneuron growth cone guidance in Drosophila

Receptor tyrosine phosphatases regulate axon guidance across the midline of the Drosophila embryo

Contact Info

Product

Resources

About