Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome.

Isogai, Masamichi; Uyeda, Ichiro; Lindsten, K.

doi:10.1099/0022-1317-79-6-1479

Cited by 39 publications

(18 citation statements)

References 24 publications

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“…This result is supported by the observation that comparable structural proteins are encoded in similar positions on NLRV S7 and S8, respectively. The sequences of the core capsid and major outer capsid proteins encoded by RBSDV S8 and S10 are homologous to the NLRV amino acid sequences [see the accompanying paper by Isogai et al (1998)]. …”

Section: Discussionmentioning

confidence: 99%

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10.

Isogai

Uyeda

Lee

1998

Journal of General Virology

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117

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The proteins encoded by rice black streaked dwarf fijivirus (RBSDV) genomic segments 7-10 (S7-S10) were characterized. Open reading frames (ORFs) from these segments were expressed as fusion proteins in Escherichia coli. Antibodies raised against the expressed products were used as probes to determine whether the viral ORFs encode structural proteins. In Western blots, antibodies to the expressed S8 and S10 products reacted with a core capsid (65 kDa) and a major outer capsid (56 kDa) protein, respectively, while none of the antibodies to S7 and S9 products reacted with structural

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Section: Discussionmentioning

confidence: 99%

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10.

Isogai

Uyeda

Lee

1998

Journal of General Virology

Self Cite

117

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“…We have recently shown that one or both lysines in motif A are essential for the triphosphatase activities of 2 (18) (see below). Although previous authors have noted similarities to NTP-binding motifs in many of these sequences (8,12,15,18), none have observed that the specific motifs shown in Fig. 1 are conserved among all genera of turreted Reoviridae.…”

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confidence: 90%

Conserved Sequence Motifs for Nucleoside Triphosphate Binding Unique to Turreted Reoviridae Members and Coltiviruses

Nibert

Kim

2004

J Virol

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“…In general, different reoviruses infect a wide range of different hosts, including mammals, birds, reptiles, fish, arthropods, fungi, protists, and plants. Reoviruses infecting plant-feeding hemipteran insects are classified into three genera: Phytoreovirus (74,75), Fijivirus (76,77), and Oryzavirus (78). Fijiviruses have 10 linear dsRNA segments encoding 12 proteins (69).…”

mentioning

confidence: 99%

Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

Nouri

Salem

Nigg

et al. 2016

J Virol

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The Asian citrus psyllid, Diaphorina citri, is the natural vector of the causal agent of Huanglongbing (HLB), or citrus greening disease. Together; HLB and D. citri represent a major threat to world citrus production. As there is no cure for HLB, insect vector management is considered one strategy to help control the disease, and D. citri viruses might be useful. In this study, we used a metagenomic approach to analyze viral sequences associated with the global population of D. citri. By sequencing small RNAs and the transcriptome coupled with bioinformatics analysis, we showed that the virus-like sequences of D. citri are diverse. We identified novel viral sequences belonging to the picornavirus superfamily, the Reoviridae, Parvoviridae, and Bunyaviridae families, and an unclassified positive-sense single-stranded RNA virus. Moreover, a Wolbachia prophage-related sequence was identified. This is the first comprehensive survey to assess the viral community from worldwide populations of an agricultural insect pest. Our results provide valuable information on new putative viruses, some of which may have the potential to be used as biocontrol agents. IMPORTANCEInsects have the most species of all animals, and are hosts to, and vectors of, a great variety of known and unknown viruses. Some of these most likely have the potential to be important fundamental and/or practical resources. In this study, we used highthroughput next-generation sequencing (NGS) technology and bioinformatics analysis to identify putative viruses associated with Diaphorina citri, the Asian citrus psyllid. D. citri is the vector of the bacterium causing Huanglongbing (HLB), currently the most serious threat to citrus worldwide. Here, we report several novel viral sequences associated with D. citri. Viruses are the most abundant microbes on our planet (1) and are found in all types of organisms. Insects are the largest and most diverse taxonomic class among animals, representing perhaps half of known animals, with more than one million species recognized worldwide (2). Insects are known to be hosts to viruses belonging to various viral taxa, including the Baculoviridae, Parvoviridae, Flaviviridae, Ascoviridae, Togaviridae, Bunyaviridae, and Rhabdoviridae (3). However, the number of currently described viral species infecting insects is relatively low compared to the number of viruses that have been discovered among prokaryotes, plants, and vertebrates. Furthermore, most of the insect viruses described to date have been discovered because of their pathogenic effects on their insect hosts or because they are pathogens of humans, other vertebrates, or economically important plants. Traditional viral detection methods that require prior knowledge of genome sequences may not be suitable for the discovery of new viruses and, in particular, viruses with a high level of genetic diversity. However, in the past decade, the development of high-throughput next-generation sequencing (NGS) technologies and bioinformatics applications has provided ne...

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Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome.

Cited by 39 publications

References 24 publications

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10.

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10.

Conserved Sequence Motifs for Nucleoside Triphosphate Binding Unique to Turreted Reoviridae Members and Coltiviruses

Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

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