Taxonomic relationships in genus<i>Fritillaria</i>(Liliaceae): Evidence from RAPD-PCR and SDS-PAGE of seed proteins

Çelebi, Ayten; Tekşen, Mehtap; Açık, Leyla; Aytaç, Zekí

doi:10.1556/abot.50.2008.3-4.11

“…As a result, the well-known APG (Angiosperm Phylogeny Group) classification system was constructed based on the latest progress in plant molecular phylogenetics (Byng et al, 2016; The Angiosperm Phylogeny Group, 1998; The Angiosperm Phylogeny Group, 2003; The Angiosperm Phylogeny Group, 2009). Although molecular markers were also used for phylogenetic inference in complicated groups (Simmons et al, 2007), only a few species of Fritillaria adopted them to explore phylogeny (Çelebi et al, 2008; Wietsma et al, 2015). Nevertheless, gene fragments, especially nrITS and cpDNA genes, gained more attention due to the rapid development of DNA sequencing (Gao et al, 2010; Kress et al, 2005; Mishra et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of Fritillaria cirrhosa D. Don and its closely related species based on complete chloroplast genomes

Chen

¹

,

Wu

²

,

Zhang

³

2019

PeerJ

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Fritillaria cirrhosa D. Don, whose bulb is used in a well-known traditional Chinese medicine to relieve cough and eliminate phlegm, is one of the most important medicinal plants of Fritillaria L. The species is widely distributed among the alpine regions in southwestern China and possesses complex morphological variations in different distributions. A series of newly related species were reported, based on obscure morphological differences. As a result, F. cirrhosa and its closely related species constitute a taxonomically complex group. However, it is difficult to accurately identify these species and reveal their phylogenetic relationships using traditional taxonomy. Molecular markers and gene fragments have been adopted but they are not able to afford sufficient phylogenetic resolution in the genus. Here, we report the complete chloroplast genome sequences of F. cirrhosa and its closely related species using next generation sequencing (NGS) technology. Eight plastid genomes ranged from 151,058 bp to 152,064 bp in length and consisted of 115 genes. Gene content, gene order, GC content, and IR/SC boundary structures were highly similar among these genomes. SSRs and five large repeat sequences were identified and the total number of them ranged from 73 to 79 and 63 to 75, respectively. Six highly divergent regions were successfully identified that could be used as potential genetic markers of Fritillaria. Phylogenetic analyses revealed that eight Fritillaria species were clustered into three clades with strong supports and F. cirrhosa was closely related to F. przewalskii and F. sinica. Overall, this study indicated that the complete chloroplast genome sequence was an efficient tool for identifying species in taxonomically complex groups and exploring their phylogenetic relationships.

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“…By resolving the resulting patterns, a semiunique profile can be garnered from a RAPD reaction. The taxonomic status of 42 taxa of Fritillaria in Turkey was examined by RAPD-PCR and seed protein analysis (Celebi et al, 2008). RAPD was also used to study the genetic diversity of four F. thunbergii cultivars, for example, Duozi (many seeds), broad-leaf, narrow-leaf, and Lingxia, and five Fritillaria species (F. thunbergii, F. cirrhosa, F. anhuiensis, F. thunbergii var.…”

Section: Molecular Biologymentioning

confidence: 99%

Phytochemical and biological research of Fritillaria medicinal resources

Hao¹

2015

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“…As a result, the well-known APG (Angiosperm Phylogeny Group) classification system was constructed based on the latest progress in plant molecular phylogenetics (Byng et al, 2016;The Angiosperm Phylogeny Group, 1998;. Although molecular markers were also used for phylogenetic inference in complicated groups (Simmons et al, 2007), only a few species of Fritillaria adopted them to explore phylogeny (Çelebi et al, 2008;Wietsma et al, 2015). Nevertheless, gene fragments, especially nrITS and cpDNA genes, gained more attention due to the rapid development of DNA sequencing (Gao et al, 2010;Kress et al, 2005;Mishra et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Phylogenetic analysis of Fritillaria cirrhosa D. Don and its closely related species based on complete chloroplast genomes (v0.1)"

2019

0

View full text Add to dashboard Cite

Fritillaria cirrhosa D. Don, whose bulb is used in a well-known traditional Chinese medicine to relieve cough and eliminate phlegm, is one of the most important medicinal plants of Fritillaria L. The species is widely distributed among the alpine regions in southwestern China and possesses complex morphological variations in different distributions. A series of newly related species were reported, based on obscure morphological differences. As a result, F. cirrhosa and its closely related species constitute a taxonomically complex group. However, it is difficult to accurately identify these species and reveal their phylogenetic relationships using traditional taxonomy. Molecular markers and gene fragments have been adopted but they are not able to afford sufficient phylogenetic resolution in the genus. Here, we report the complete chloroplast genome sequences of F. cirrhosa and its closely related species using next generation sequencing (NGS) technology. Eight plastid genomes ranged from 151,058 bp to 152,064 bp in length and consisted of 115 genes. Gene content, gene order, GC content, and IR/SC boundary structures were highly similar among these genomes. SSRs and five large repeat sequences were identified and the total number of them ranged from 73 to 79 and 63 to 75, respectively. Six highly divergent regions were successfully identified that could be used as potential genetic markers of Fritillaria. Phylogenetic analyses revealed that eight Fritillaria species were clustered into three clades with strong supports and F. cirrhosa was closely related to F. przewalskii and F. sinica. Overall, this study indicated that the complete chloroplast genome sequence was an efficient tool for identifying species in taxonomically complex groups and exploring their phylogenetic relationships.

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Taxonomic relationships in genusFritillaria(Liliaceae): Evidence from RAPD-PCR and SDS-PAGE of seed proteins

Cited by 8 publications

References 11 publications

Phylogenetic analysis of Fritillaria cirrhosa D. Don and its closely related species based on complete chloroplast genomes

Phylogenetic analysis of Fritillaria cirrhosa D. Don and its closely related species based on complete chloroplast genomes

Phytochemical and biological research of Fritillaria medicinal resources

Peer Review #2 of "Phylogenetic analysis of Fritillaria cirrhosa D. Don and its closely related species based on complete chloroplast genomes (v0.1)"

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