Taxonomy and Identification of the
            <i>Burkholderia cepacia</i>
            Complex

Coenye, Tom; Vandamme, Peter; Govan, John R. W.; LiPuma, John J.

doi:10.1128/jcm.39.10.3427-3436.2001

Cited by 388 publications

(361 citation statements)

References 122 publications

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“…The Burkholderia cepacia complex (Bcc) is a diverse group of bacteria (Coenye et al, 2001c;Vandamme et al, 1997) which are ubiquitous in nature (Coenye & Vandamme, 2003). Some Bcc strains are recognized for their beneficial properties as plant-growth promoters, bioremediators and biocontrol strains (Parke & Gurian-Sherman, 2001), whereas others are notorious as pathogens causing infections in plants and immunocompromised individuals such as cystic fibrosis patients (Coenye & Vandamme, 2003;Mahenthiralingam et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Vanlaere

LiPuma

Baldwin

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

223

143

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The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach, The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA-DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3(T) =LMG 24064(T) =CCUG 54555(T)), Burkholderia diffusa sp. nov. (type strain AU1075(T) =LMG 24065(T) =CCUG 54558(T)), Burkholderia arboris sp. nov. (type strain ES0263A(T) =LMG 24066(T) =CCUG 54561(T)), Burkholderia seminalis sp. nov. (type strain AU0475(T) =LMG 24067(T) =CCUG 54564 (T)) and Burkholderia metallica sp. nov. (type strain AU0553(T) =LMG 24068(T) =CCUG 54567(T)). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc

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Section: Introductionmentioning

confidence: 99%

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Vanlaere

LiPuma

Baldwin

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

223

143

View full text Add to dashboard Cite

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“…S2). It is known that phylogenetic analysis based exclusively on the 16S rRNA gene often does not reflect the correct taxonomic position of a species (Coenye et al, 2001;Martens et al, 2007;Menna et al, 2009;Wang & Martínez-Romero, 2000). We tested whether the similarity between PRF 81 T and R. multihospitium also included other parts of the ribosomal (rrn) operon by comparing the sequences of their 16S-23S intergenic spacer (IGS) regions.…”

mentioning

confidence: 99%

Rhizobium freirei sp. nov., a symbiont of Phaseolus vulgaris that is very effective at fixing nitrogen

Dall’Agnol

Ribeiro

Ormeño‐Orrillo

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Common bean (Phaseolus vulgaris L.) can establish symbiotic associations with several Rhizobium species; however, the effectiveness of most strains at fixing nitrogen under field conditions is very low. PRF 81 T is a very effective strain, usually referred to as Rhizobium tropici and used successfully in thousands of doses of commercial inoculants for the common bean crop in Brazil; it has shown high rates of nitrogen fixation in all areas representative of the crop in the country. Here, we present results that indicate that PRF 81 T , although it belongs to the 'R. tropici group', which includes 10 Rhizobium species, R. tropici, R. leucaenae, R. lusitanum, R. multihospitium, R. miluonense, R. hainanense, R. calliandrae, R. mayense, R. jaguaris and R. rhizogenes, represents a novel species. Several morpho-physiological traits differentiated PRF 81 T from related species. Differences were also confirmed in the analysis of rep-PCR (sharing less than 45 % similarity with the other species), MLSA with recA, atpD and rpoB genes, and DNA-DNA hybridization. The novel species, for which we propose the name Rhizobium freirei sp. nov., is able to establish effective root nodule symbioses with Phaseolus vulgaris, Leucaena leucocephala, Leucaena esculenta, Crotalaria juncea and Macroptilium atropurpureum. The type strain is PRF 81 T (5CNPSo 122 T 5SEMIA 4080 T 5IPR-Pv81 T 5WDCM 440 T ).

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“…A variety of different classes of compounds like N-containing heterocycles, unusual amino acids, peptides, and glycolipids have been isolated from pseudomonads [5ϳ7]. Pseudomonas species belonging to rRNA group II of the genus Pseudomonas have been changed to the new genus Burkholderia [8].…”

Section: Introductionmentioning

confidence: 99%

Novel Oxidized Derivatives of Antifungal Pyrrolnitrin from the Bacterium Burkholderia cepacia K87

et al. 2008

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The screening of antifungal active compounds from the fermentation extracts of soil-borne bacterium Burkholderia cepacia K87 afforded pyrrolnitrin (1) and two new pyrrolnitrin analogs, 3-chloro-4-(3-chloro-2-nitrophenyl)-5-methoxy-3-pyrrolin-2-one (2) and 4-chloro-3-(3-chloro-2-nitrophenyl)-5-methoxy-3-pyrrolin-2-one (3). Pyrrolnitrin showed strong antifungal activity against Rhizoctonia solani but the analogs (2 and 3) were found to be marginally active. The isolates, 2 and 3, are believed to be biodegraded derivatives of pyrrolnitrin.

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Taxonomy and Identification of the Burkholderia cepacia Complex

Cited by 388 publications

References 122 publications

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Rhizobium freirei sp. nov., a symbiont of Phaseolus vulgaris that is very effective at fixing nitrogen

Novel Oxidized Derivatives of Antifungal Pyrrolnitrin from the Bacterium Burkholderia cepacia K87

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