TCF21 regulates miR-10a-5p/LIN28B signaling to block the proliferation and invasion of melanoma cells

Abstract

Background and aim Some research has suggested that miRNA-10a (miR-10a-5p) had an inhibitory function in proliferation and invasion of cancers. Whereas the role of miR-10a-5p in melanoma has not been fully explored. This study aims to confirm LIN28B as the targeted gene of miR-10a-5p which was explored in melanoma cells. In addition, upstream regulatory molecule of miR-10a-5p was also investigated in melanoma cells. Methods Real-time Quantitative polymerase chain reaction (RT-qPCR) was adopted to analyze miR-10a-5p expression level in melanoma and the normal human epidermal melanocyte cells. Several biological assays were performed to evaluate miR-10a-5p influences on cell proliferation, migration and invasion ability in A375 and B16-F10 cells. Gene prediction of miRNA targeting and a dual luciferase assay were applied to assess miR-10a-5p-targeted LIN28B. Western blot assessed the impacts of miR-10a-5p on the protein expression of LIN28B. Western blot analyzed the TCF21 effects on the expression of LIN28B and RT-qPCR assessed the influence of TCF21 on the expression level of miRNA-10a. In addition, Chromatin Immunoprecipitation (ChIP) Assay and JASPAR databases were employed to explore the regulatory relationship between TCF21 and miR-10a-5p. Results We discovered that miR-10a-5p expression was lower in melanoma cells and high expression of miR-10a-5p suppressed the proliferation, migration and invasion abilities of melanoma cells. We also discovered that miR-10a-5p targeted the LIN28B mRNA 3′UTR area and diminished LIN28B protein expression. We found that LIN28B expression was strongly decreased by TCF21 upregulation in the two melanoma cells. The qRT-PCR assay showed that miR-10a-5p expression level was obviously boosted by increased TCF21 expression. The results also demonstrated that TCF21 directly regulated miR-10a-5p at transcript levels. Conclusion TCF21 induced miRNA-10a targeting LIN28B could affect the progression and growth of melanoma.

Transcriptomic analyses of ovarian clear-cell carcinoma with concurrent endometriosis

“…In vitro studies overexpressing miR-10a-5p showed potentially cancer type specific effects on cellular proliferation. For example, overexpression of miR-10a in melanoma, acute myeloid leukemia, and laryngeal squamous cell carcinoma cells decreased cellular proliferation in these cancers ( 108 – 110 ).…”

miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene

“…Thus, we delved into the molecular mechanism of miR-10a-5p in porcine fat metabolism, revealing the role of miR-10a-5p in preadipocyte differentiation by targeting the KLF11 gene. Previous research has shown miR-10a-5p's involvement in inhibiting chicken granulosa cell proliferation by targeting MAPRE1 to suppress CDK2, as well as suppressing melanoma cell proliferation and migration [32,33]. It also impacts keratinocyte proliferation and directly targets hyaluronan synthase 3 [34].…”

MicroRNA as a Diagnostic Tool, Therapeutic Target and Potential Biomarker in Cutaneous Malignant Melanoma Detection—Narrative Review

“…MiR-10a-5p is directly regulated by TCF21, which binds to its promoter region. The TCF21/miR-10a-5p/LIN28B pathway could be targeted by a repressor in a new melanoma treatment in order to stop melanoma progression [ 95 ].…”