Technique for rapid in vitro multiplication of Vitis vinifera L. cultivars

In vitro Clonal Multiplication of Two Grape (Vitis spp.) Rootstock Genotypes

“…45.8 days. Recently, similar strategy has been used for acclimation of two V. vinifera grape varieties (Singh et al 2004). They observed that tissue culture raised plantlets grew vigorously compared to plants derived from cuttings.…”

“…They demonstrated that Kin was more effective than 2-ip and BAP for culture establishment in a wild grape genotype. However, earlier studies recommended BAP as an effective cytokinin for grapevine culture establishment (Novak and Juvova 1982/83, Singh et al 2004). Sudarsono and Goldy (1991) on the other hand, reported that thidiazuron (TDZ) alone or in combination with BA or Kin was effective for in vitro culture establishment in Vitis rotundifolia.…”

“…IBA has been reported to have favoured root induction in several species including kiwifruit (Monette 1986), walnut (Tang et al 2000) and grape (Mhatre et al 2000, Singh et al 2004, Barreto and Nookaraju 2007. Two IBA concentrations (2 and 4 mg/l) used in this study, differed in their effects on the rooting parameters.…”

“…The potential for integration of micropropagation to viticulture is numerous. Several reports have elucidated micropropagation of V. vinifera (Chee and Pool 1982, Reisch 1986, Singh et al 2004) as well as muscadine grapes (Lee and Wetzstein 1990, Gray and Benton 1991, Sudarsono and Goldy 1991, Thies and Graves 1992, Torregrosa and Bouquet 1995, Roubelakis-Angelakis 2001. However, different species may vary in their performance under in vitro conditions (Poudel et al 2005, Hassan andZayed 2018).…”

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“…Bigger concentrations of BAP exhibited shooting inall the varieties increase in the concentration of BAP the number of shoots decreased worked on 'Summit' variety of grapes and observed strong inhibition of shoot elongation with almost no elongated shoots on 10 µM BA concentrations and BAP has been reported to be the most commonly used cytokinin for shoot induction and multiplication in grape tissue culture [13,15,25]. The effect of BAP has also been reported beneficial for multiple shoots induction in grape by Alizadehet al BAP has been known for breaking apical dominance and enhancement of axillary branching, but higher levels may sometimes suppress growth and is even toxic to the significant results for three varieties (Red Globe, Autumn Royal and Thompson) among the four tried for both the shoot induction and shoot reported micropropagation of Vitis vinifera cvNortons in MS medium supplemented with 4.0 µM BA and 0.5mg/l Thiamine [28].…”