Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide

Sakamoto, Chiaki; Okada, Yuji; Kanazawa, Hideko; Ayano, Eri; Nishimura, Takuya; Ando, Masayuki; Kikuchi, Akihiko; Okano, Teruo

doi:10.1016/j.chroma.2003.09.010

Cited by 74 publications

(44 citation statements)

References 16 publications

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“…19,26,28 Polymerization was conducted using AIBN as an initiator and MPA as chain-transfer agents. Poly(NIPAAm-co-BMA) (IB) with a feed ratio of NIPAAm/BMA molar ratio fixed to 95/5, poly(NIPAAm-co-BMA-co-DMAPAAm) (IBD) with a feed ratio of DMAPAAm 5.0 mol%, and NIPAAm/BMA with a molar ratio fixed to 95/5 were synthesized in DMF with AIBN and MPA.…”

Section: Preparation Of Pnipaam-modified Silicamentioning

confidence: 99%

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

et al. 2006

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Section: Preparation Of Pnipaam-modified Silicamentioning

confidence: 99%

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

et al. 2006

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“…22,41 As the temperature was raised, the surface property of the stationary phase changed from hydrophilic to hydrophobic. The retention on the PNIPAAm-modified stationary phase remarkably changed upon changing the temperature.…”

Section: Temperature-responsive Chromatography Utilizing Smart Polymersmentioning

confidence: 99%

Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Ayano

Kanazawa

2014

ANAL. SCI.

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“…We have been investigating temperature-responsive chromatography, in which a thermo-responsive polymer such as poly(N-isopropylacrylamide) (PNIPAAm) is used as the stationary phase [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. PNIPAAm exhibits thermally reversible solubility changes in aqueous solutions in response to temperature changes across its lower critical solution temperature (LCST).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Psychoactive Drugs by Temperature-Responsive Chromatography

et al. 2017

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Temperature-responsive chromatography, in which the characteristics of the stationary phase can be controlled by varying the column temperature with only an aqueous eluent, was applied to the analysis of psychoactive drugs. Temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm)-based copolymers were synthesized with n-butyl methacrylate (BMA) and N-acryloyl L-phenylalanine methyl ester (Phe-OMe) as a comonomer. These polymers were grafted onto aminopropyl silica and used as the stationary phase. Seven psychoactive drugs could be separated simultaneously by the column, including BMA. Among the seven drugs, for triazolam, which has a triazole ring in its structure, the order of elution changed on changing the column temperature. The phenomenon could be explained by NH-π interactions between the NH groups of PNIPAAm and π electrons of the triazole ring. The conformational changes of PNIPAAm altered the degree of exposure of the NH groups and would affect the elution order of the analytes. To enhance the molecular recognition ability for the triazole ring, the column containing Phe-OMe was used. Noticeable changes in the retention factors occurred due to the additional π-π interactions between the phenyl moieties of Phe-OMe and the triazole ring. The effects of the π-π interactions were also altered by the changes in column temperature. This chromatographic system, which needs no toxic organic solvent, offers an effective way to determine the causes of addiction in medical institutions.

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Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide

Cited by 74 publications

References 16 publications

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Analysis of Psychoactive Drugs by Temperature-Responsive Chromatography

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