Temperature dependence of intrinsic membrane properties and synaptic potentials in hippocampal CA1 neurons in vitro

Thompson, S. M.; Masukawa, LM; Prince, D. A.

doi:10.1523/jneurosci.05-03-00817.1985

Cited by 277 publications

(200 citation statements)

References 15 publications

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“…Reduced input resistance at elevated temperature is a consistent finding in a variety of neuronal types (Cao and Oertel 2005;Griffin and Boulant 1995;Klee et al 1974;Lee et al 2005;Thompson et al 1985;Volgushev et al 2000). This is also the case for SDH neurons in general (Table 1); however, when neurons were separated by discharge category the reduction in input resistance was not uniform.…”

Section: Membrane Propertiessupporting

confidence: 65%

“…In contrast, cooling mouse cochlear neurons leads to hyperpolarization and heating leads to depolarization (Cao and Oertel 2005). Finally, studies in hippocampal CA1, hypothalamic, and neocortical pyramidal neurons suggest temperature has little or no effect on RMP (Griffin and Boulant 1995; Lee et al 2005; Thompson et al 1985). In mouse SDH neurons we observed a modest depolarization of RMP (ϳ3 mV) at elevated temperature (Table 1) in population comparisons.…”

Section: Membrane Propertiesmentioning

confidence: 55%

“…The effects of lowered temperature, however, may be countered by the slowing of biochemical reactions and intracellular processes that follow Ca 2ϩ influx. For example, electrogenic pumps (e.g., Na ϩ / K ϩ -ATPase) are highly temperature sensitive (Q 10 Ͼ2), and show reduced activity as temperature decreases (Thompson et al 1985). Future studies, at elevated temperatures, are required to fully understand the net result of altered Ca 2ϩ entry, electrogenic pump activity, and the downstream effects, in vivo.…”

Section: Comparison Of In Vitro and In Vivo Ap Dischargementioning

confidence: 99%

“…In cases where in vitro experiments have been repeated at higher temperatures, many phenomena are markedly different (Cao and Oertel 2005;Lee et al 2005;Micheva and Smith 2005). For example, neurons in the brain stem (Borst and Sakmann 1998;Cao and Oertel 2005), hypothalamus (Griffin and Boulant 1995), hippocampus (Thompson et al 1985), and cortex (Lee et al 2005;Volgushev et al 2000) have altered input resistance, action potential (AP) amplitude, and AP width at elevated temperature (Ն32°C).…”

Section: Introductionmentioning

confidence: 99%

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Recording Temperature Affects the Excitability of Mouse Superficial Dorsal Horn Neurons, In Vitro

Graham

Brichta²,

Callister³

2008

Journal of Neurophysiology

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Graham BA, Brichta AM, Callister RJ. Recording temperature affects the excitability of mouse superficial dorsal horn neurons, in vitro. J Neurophysiol 99: 2048 -2059, 2008. First published February 20, 2008 doi:10.1152/jn.01176.2007. Superficial dorsal horn (SDH) neurons in laminae I-II of the spinal cord play an important role in processing noxious stimuli. These neurons represent a heterogeneous population and are divided into various categories according to their action potential (AP) discharge during depolarizing current injection. We recently developed an in vivo mouse preparation to examine functional aspects of nociceptive processing and AP discharge in SDH neurons and to extend investigation of pain mechanisms to the genetic level of analysis. Not surprisingly, some in vivo data obtained at body temperature (37°C) differed from those generated at room temperature (22°C) in spinal cord slices. In the current study we examine how temperature influences SDH neuron properties by making recordings at 22 and 32°C in transverse spinal cord slices prepared from L3-L5 segments of adult mice (C57Bl/6). Patch-clamp recordings (KCH 3 SO 4 internal) were made from visualized SDH neurons. At elevated temperature all SDH neurons had reduced input resistance and smaller, briefer APs. Resting membrane potential and AP afterhyperpolarization amplitude were temperature sensitive only in subsets of the SDH population. Notably, elevated temperature increased the prevalence of neurons that did not discharge APs during current injection. These reluctant firing neurons expressed a rapid A-type potassium current, which is enhanced at higher temperatures and thus restrains AP discharge. When compared with previously published whole cell recordings obtained in vivo (37°C) our results suggest that, on balance, in vitro data collected at elevated temperature more closely resemble data collected under in vivo conditions.

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Section: Membrane Propertiessupporting

confidence: 65%

Section: Membrane Propertiesmentioning

confidence: 55%

Section: Comparison Of In Vitro and In Vivo Ap Dischargementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recording Temperature Affects the Excitability of Mouse Superficial Dorsal Horn Neurons, In Vitro

Graham

Brichta²,

Callister³

2008

Journal of Neurophysiology

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“…Change in brain temperature is a factor that affects various neural functions ranging from the activity of ionic channels and receptor sensitivity (Thompson et al, 1985;Rosen, 2001) to such global neuronal alterations as release and uptake of neurotransmitters (Andersen and Moser, 1995;Xie et al, 2000). Thus, the METH-induced metabolic activation suggested by our temperature measurements could be a "common denominator" for various abnormalities in neural functions (i.e., thermal stress, oxidative stress, abnormal transmitter release, decrease in ATP, etc.)…”

Section: Meth Induces Pathological Metabolic Neural Activation and Hymentioning

confidence: 85%

Brain Hyperthermia Is Induced by Methamphetamine and Exacerbated by Social Interaction

2003

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Hyperthermia is a symptom of methamphetamine (METH) intoxication and a factor implicated in neurotoxicity during chronic METH use. To characterize the thermic response to METH, it was injected once daily into rats at increasing doses (0, 1, 3, and 9 mg/kg, s.c.) while brain [nucleus accumbens (NAcc), hippocampus] and body (deep temporal muscle) temperatures were continuously monitored. METH produced dose-dependent hyperthermia, with brain structures (especially the NAcc) showing a more rapid and pronounced temperature increase than the muscle. At the highest dose, brain and body temperatures increased 3.5-4.0°C above basal levels and remained elevated for 3-5 hr. Stressful and other high-activity situations such as interaction with a conspecific female are also known to induce a significant hyperthermic response in the rat. A combination of social interaction and METH administration was tested for additive effects. Male rats were exposed daily to a conspecific female for a total of 120 min, and METH was injected at the same doses 30 min after the initial contact with the female. An initial hyperthermic response (ϳ1.5°C) to social interaction was followed by a large and prolonged hyperthermic response (3.5-5.0°C, 5-7 hr at 9 mg/kg) to METH, which was again stronger in brain structures (especially in the NAcc) than in the muscle. Although the combined effect of the hyperthermic events was not additive, METH administration during social interaction produced stronger and longer-lasting increases in brain and body temperature than that induced by drug alone, heating the brain in some animals near its biological limit (Ͼ41°C).

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Dissociation between synaptic depression and block of long-term depression induced by raising the temperature in rat hippocampal slices

Young

Luo

Shen

et al. 2001

Synapse

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Temperature dependence of intrinsic membrane properties and synaptic potentials in hippocampal CA1 neurons in vitro

Cited by 277 publications

References 15 publications

Recording Temperature Affects the Excitability of Mouse Superficial Dorsal Horn Neurons, In Vitro

Recording Temperature Affects the Excitability of Mouse Superficial Dorsal Horn Neurons, In Vitro

Brain Hyperthermia Is Induced by Methamphetamine and Exacerbated by Social Interaction

Dissociation between synaptic depression and block of long-term depression induced by raising the temperature in rat hippocampal slices

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