Temperature-Dependent Alterations in Sugar Transport in Cells Infected by a Temperature-Sensitive Mutant of Rous Sarcoma Virus

Martin, G S; Venuta, Salvatore; Weber, Michael J.; Rubin, H. B.

doi:10.1073/pnas.68.11.2739

Cited by 145 publications

(68 citation statements)

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“…It is remarkable how rapidly (within 24 hr) the levels of multiple-length mtDNA change after temperature shifts from 36 to 41 or 41 to 360 (Table 3). Martin et al (9) have shown that already 6 hr after temperature shifts to 360, and 12-18 hr after shifts to 410, the rate of deoxyglucose transport reaches the new level. The early changes in oligomer levels are not known, but it is possible that an initial overstimulation of the processes leading to changes in oligomer levels occurs, so that after one cell division (24 hr (9) as well as alterations of glycoprotein (10) content.…”

Section: Discussionmentioning

confidence: 99%

“…Martin et al (9) have shown that already 6 hr after temperature shifts to 360, and 12-18 hr after shifts to 410, the rate of deoxyglucose transport reaches the new level. The early changes in oligomer levels are not known, but it is possible that an initial overstimulation of the processes leading to changes in oligomer levels occurs, so that after one cell division (24 hr (9) as well as alterations of glycoprotein (10) content. The differences in glycoprotein patterns are not confined to surface membranes, but also include mitochondrial and other intracellular membranes (23).…”

Section: Discussionmentioning

confidence: 99%

“…Cells were plated in roller bottles (Bellco, growth-area 690 cm2) at 2 X 107 cells per bottle. Cultures were infected 24-48 hr after seeding with either the Schmidt-Ruppin strain of Rous sarcoma virus, subgroup A, or the temperature-sensitive mutant of this virus, T5, (9,12). After 1 hr, the cells were incubated at 360 in 50 ml of medium.…”

Section: Methodsmentioning

confidence: 99%

“…The present study utilizes an ideally controlled experimental system of chick-embryo fibroblasts made malignant by infection with temperature-sensitive mutants of oncogenic viruses. Upon infection with the thermosensitive mutant T5 of Rous sarcoma virus (9), cell transformation becomes manifested morphologically and functionally at 360, the permissive temperature. The cells round up, grow in soft agar, and have an increased rate of deoxyglucose uptake (9) and an altered glycoprotein pattern (10).…”

mentioning

confidence: 99%

“…Upon infection with the thermosensitive mutant T5 of Rous sarcoma virus (9), cell transformation becomes manifested morphologically and functionally at 360, the permissive temperature. The cells round up, grow in soft agar, and have an increased rate of deoxyglucose uptake (9) and an altered glycoprotein pattern (10). At 410, the nonpermissive temperature, the cells have the phenotypic appearance and behavior of normal cells.…”

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confidence: 99%

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Temperature-Dependent Formation of Dimers and Oligomers of Mitochondrial DNA in Cells Transformed by a Thermosensitive Mutant of Rous Sarcoma Virus

Nass

1973

Proc. Natl. Acad. Sci. U.S.A.

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In chick-embryo fibroblasts infected by T5, a temperature-sensitive mutant of Schmidt-Ruppin Rous sarcoma virus, the level of catenated dimeric and oligomeric mitochondrial DNA is temperature-dependent and correlates with the phenotypic manifestation of transformation. At the permissive temperature (360), where transformation is expressed, the 2-to 3-fold elevated level characteristic of cells transformed by the wild-type Rous sarcoma virus was observed, but at the nonpermissive temperature (410), at which cells appear normal and behave normally, the oligomer level was characteristic of uninfected cells. Temperature shifts from 41 to 360 and vice versa resulted in phenotypic reversion and reversals of the respective levels of multiple-length DNA. Cellular growth rates were not altered. Treatment of control cells with cycloheximide resulted in a 5-fold increase of oligomeric DNA, whereas exposure to 9-j8-D-arabinofuranosyladenine had little effect. With both inhibitors, nuclear but not mitochondrial DNA synthesis was inhibited. The possible relation of formation of oligomeric mitochondrial DNA to alterations in the mitochondrial membranes of transformed cells and to inhibition of protein synthesis in the cytoplasm is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Temperature-Dependent Formation of Dimers and Oligomers of Mitochondrial DNA in Cells Transformed by a Thermosensitive Mutant of Rous Sarcoma Virus

Nass

1973

Proc. Natl. Acad. Sci. U.S.A.

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Temperature‐dependent alterations in 2‐deoxyglucose uptake in rat cells transformed by a cold‐sensitive murine sarcoma virus mutant

May

Somers

Kit

1973

Intl Journal of Cancer

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Cells transformed by Moloney sarcoma virus ( M S V ) take up 2-deoxyglucose at a faster rate at 39" C than uninjkcted or Moloney leukemia virus (MoLV)-infected normal rat kidney ( N R K ) cells. In a sarcoma-virus-transformed cell line, NRK (MSV-lb), whose transformed phenotype is expressed at 39" C (permissive) but not at 33" C (non-permissive), the stimulation of 2-deoxyglucose uptake at 39" C is temperature dependent: the increase is observed when cells are grown at 39" C but not at 33" C. When these cells were shifed from the non-permissive to the permissive temperature, the uptake increased ,from a rate near that of uninfected cells to a rate half that of NRK cells infected with Moloney sarcoma-leukemia complex (MSVMoLV). The reverse change occurred when the cells were shifted from the permissive to the non-permissive temperature. Thus in a sarcoma virus-infected rat cell line where the maintenance of the transformed state is dependent on a cold-sensitive viral function, the uptake of 2-deoxyglucose is reduced in the cold-sensitive cells and correlated with the expression of the transformed phenotype.Cells transformed by either avian or murine sarcoma viruses show characteristic morphological alterations. Concomitant with the morphological changes was the finding that sarcoma viruses take up glucose analogs, such as 2-deoxyglucose, at a faster rate than uninfected cells (Hatanaka and Gilden, 1970;Hatanaka and Hanafusa, 1970). More recently the increased uptake of 2-deoxyglucose by transformed cells has been used to study the effect of temperature shifts on cells infected by temperature-sensitive avian sarcoma virus mutants (Kawai and Hanafusa, 1971; Martin et al., 1971;Bader, 1972).We have recently reported the isolation of a temperature-dependent MSV-transformed normal rat kidney cell line, designated NRK(MSV-lb), which expressed its transformed phenotype at 39" C but not at 33" C as judged by the criteria of morphological changes (Somers and Kit, 1973). These morphological studies have now been extended to include a study on the effect of temperature shifts on the uptake of labelled 2-deoxyglucose. MATERIAL AND METHODS CellsCell lines used in this study have been previously described and characterized (Somers and Kit, 1971, 1973), and some of their properties are listed in Table I. One of the lines of transformed normal rat kidney (NRK) cells, that is NRK(MSV-I), produced a virus, MSV-1 (NRK), that could transform NRK cells but failed to produce progeny virus in the newly infected cells. Focus-derived NRK cells transformed by MSV-1 (NRK) were designated NRK(MSV-1 b), and the maintenance of the transformed state was shown to be cold-sensitive (Somers and Kit, 1973).

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Kinetics of uptake of 2‐deoxy‐d‐glucose and 2‐aminoisobutyric acid in chemically transformed cells

Kuroki

Yamakawa

1974

Intl Journal of Cancer

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Kinetics of 2-deoxy-D-glucose and 2-aminoisobutyric acid uptake in chemically transformed hamster embryo cells and BA LB 3T3 cells were investigated. Maximum velocity of the glucose uptake in chemically transformed cells increased 1.5 to 2.8-fold over controls in Vmax without a detectable change in apparent Km. In uptake of the amino acid there was a slight change in Vmax and unchanged Km with chemically transformed cells, as compared to untransformed cells. Hexokinase activity showed a slight change after chemical transformation.

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Temperature-Dependent Alterations in Sugar Transport in Cells Infected by a Temperature-Sensitive Mutant of Rous Sarcoma Virus

Cited by 145 publications

References 7 publications

Temperature-Dependent Formation of Dimers and Oligomers of Mitochondrial DNA in Cells Transformed by a Thermosensitive Mutant of Rous Sarcoma Virus

Temperature-Dependent Formation of Dimers and Oligomers of Mitochondrial DNA in Cells Transformed by a Thermosensitive Mutant of Rous Sarcoma Virus

Temperature‐dependent alterations in 2‐deoxyglucose uptake in rat cells transformed by a cold‐sensitive murine sarcoma virus mutant

Kinetics of uptake of 2‐deoxy‐d‐glucose and 2‐aminoisobutyric acid in chemically transformed cells

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