Temperature-dependent fluorescence intermittency for single molecules of violamine R in poly(vinyl alcohol)

Sluss, Daniel R. B.; Bingham, Chris; Burr, Mimosa; Bott, Eric D.; Riley, Erin A.; Reid, Philip J.

doi:10.1039/b909076b

Cited by 11 publications

(11 citation statements)

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“…[48][49][50][51][52][53] Previous studies of emission intermittency and molecular rotation have pointed to domain formation as the origin of invariant properties of the included molecule for temperatures spanning T g of the host. 11,32,48,49,51,[54][55][56] Our results are consistent with VR residing in semi-stable structural domains that exchange slowly compared to the time scale of blinking. The length scale of these domains must be large enough that the separation between VR and the electron-trapping sites on the polymer is not dramatically altered as the temperature transitions through T g .…”

Section: On-and Off-interval Analysissupporting

confidence: 83%

“…A maximum of 12 intensity states were employed in our analysis, though in previous studies we employed a maximum of six states. 32 The increased number of states used in this analysis provided a more accurate determination of the true number of states. For instance, about 30% of the KAP data were fit by fewer than 12 states, whereas all six states would be needed previously.…”

Section: Resultsmentioning

confidence: 99%

“…Single-molecule emission was collected with an inverted scanning fluorescence confocal microscope, described in detail elsewhere. 31,32 For polymer measurements, the 532 nm (Spectra Physics Excelsior) excitation field was circularly polarized using a l/4 wave plate to allow for excitation of all in-plane dye orientations. Excitation powers of o150 nW were employed.…”

Section: Single Molecule Confocal Microscopymentioning

confidence: 99%

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Two mechanisms for fluorescence intermittency of single violamine R molecules

Riley

Bingham

Bott

et al. 2011

Phys. Chem. Chem. Phys.

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The environment and temperature-dependent photoluminescence (PL) intermittency or "blinking" demonstrated by single violamine R (VR) molecules is investigated in two environments: poly(vinyl alcohol) (PVOH) and single crystals of potassium acid phthalate (KAP). In addition, temperatures ranging from 23 °C to 85 °C are studied, spanning the glass-transition temperature of PVOH (T(g) = 72 °C). The PL intermittency exhibited by VR is analyzed using probability histograms of emissive and non-emissive periods. In both PVOH and KAP, these histograms are best fit by a power law, consistent with the kinetics for dark state production and decay being dispersed as observed in previous studies. However, these systems have different temperature dependences, signifying two different blinking mechanisms for VR. In PVOH, the on- and off-event probability histograms do not vary with temperature, consistent with electron transfer via tunneling between VR and the polymer. In KAP the same histograms are temperature dependent, and show that blinking slows down at higher temperatures. This result is inconsistent with an electron-transfer process being responsible for blinking. Instead, a non-adiabatic proton-transfer between VR and KAP is presented as a model consistent with this temperature dependence. In summary, the results presented here demonstrate that for a given luminophore, the photochemical processes responsible for PL intermittency can change with environment.

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Section: On-and Off-interval Analysissupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Single Molecule Confocal Microscopymentioning

confidence: 99%

See 1 more Smart Citation

Two mechanisms for fluorescence intermittency of single violamine R molecules

Riley

Bingham

Bott

et al. 2011

Phys. Chem. Chem. Phys.

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“…Comparative studies of violamine R (VR) emission in PVOH and KAP found that the temperature dependence of VR PI was markedly different in these two environments. Specifically, in PVOH a very modest temperature dependence of the power-law exponents describing emissive and non-emissive event durations was observed [ 114 ]. In contrast, for VR in KAP both the on and off -event durations increased with increased temperature.…”

Section: Photoluminescence Intermittency In Organic Luminophoresmentioning

confidence: 99%

Photoluminescence Intermittency from Single Quantum Dots to Organic Molecules: Emerging Themes

Riley

Hess

Reid

2012

IJMS

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Recent experimental and theoretical studies of photoluminescence intermittency (PI) or “blinking” exhibited by single core/shell quantum dots and single organic luminophores are reviewed. For quantum dots, a discussion of early models describing the origin of PI in these materials and recent challenges to these models are presented. For organic luminophores the role of electron transfer, proton transfer and other photophysical processes in PI are discussed. Finally, new experimental and data analysis methods are outlined that promise to be instrumental in future discoveries regarding the origin(s) of PI exhibited by single emitters.

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“…A proposed mechanism for such a fluorescence intermittency is the formation of a radical dark state [119]. There have been several studies on the topic of blinking, but there is only a limited amount of room-temperature and low-temperature data available and many questions remain open [120][121][122][123][124][125][126].…”

Section: Super-resolution Microscopy Based On Single Molecule Localizmentioning

confidence: 99%

Light microscopy: an ongoing contemporary revolution

Weisenburger

Sandoghdar

2015

Contemporary Physics

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Optical microscopy is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical studies of individual molecules and resolving sub-wavelength structures were not feasible. The Nobel Prize in 2014 for superresolution fluorescence microscopy marks a clear recognition that the old beliefs have to be revisited. In this article, we present a critical overview of various recent developments in optical microscopy. In addition to the popular super-resolution fluorescence methods, we discuss the prospects of various other techniques and imaging contrasts and consider some of the fundamental and practical challenges that lie ahead.

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Temperature-dependent fluorescence intermittency for single molecules of violamine R in poly(vinyl alcohol)

Cited by 11 publications

References 50 publications

Two mechanisms for fluorescence intermittency of single violamine R molecules

Two mechanisms for fluorescence intermittency of single violamine R molecules

Photoluminescence Intermittency from Single Quantum Dots to Organic Molecules: Emerging Themes

Light microscopy: an ongoing contemporary revolution

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