Temperature-Dependent Measurements of a Flashlamp-Pumped Dye Laser

Huth, B. G.; Farmer, G. I.; Kagan, M. R.

doi:10.1063/1.1657365

Cited by 16 publications

(8 citation statements)

References 16 publications

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“…Due to the flexibility of the diethylamino groups, 5 also exhibits at emperature-dependent fluorescence quantum yield. [26] A bathochromic-shift of the emission peak can be obtained by methylation or ethylation, and this improves fluorophore properties for live-cell and/or single-molecule fluorescence imaging.A tt he same time at wisted internal charge transfer (TICT) state [24,27] that decreases quantum efficiency is produced;a ll rhodamines modified in this way have al ower quantum yield than fluorophore 1 (l em = 520 nm). In order to mitigate TICT and improve fluorescence properties,L avis group constructed neoclassical rhodamine dyes 7-10 [28] by replacing the amidogens of rhodamine 110 with nitrogenheterocyclic alkanes of various sizes.T he N-heterocyclic fluorophores have longer wavelength emissions in the region of 570-590 nm;t his contrasts with the methylated or ethylated fluorophores,w hich have emission between 530 and 570 nm.…”

Section: Classical Rhodamine Dyesmentioning

confidence: 99%

Hybrid Rhodamine Fluorophores in the Visible/NIR Region for Biological Imaging

et al. 2019

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Fluorophores and probes are invaluable for the visualization of the location and dynamics of gene expression, protein expression, and molecular interactions in complex living systems. Rhodamine dyes are often used as scaffolds in biological labeling and turn‐on fluorescence imaging. To date, their absorption and emission spectra have been expanded to cover the entire near‐infrared region (650–950 nm), which provides a more suitable optical window for monitoring biomolecular production, trafficking, and localization in real time. This review summarizes the development of rhodamine fluorophores since their discovery and provides strategies for modulating their absorption and emission spectra to generate specific bathochromic‐shifts. We also explain how larger Stokes shifts and dual‐emissions can be obtained from hybrid rhodamine dyes. These hybrid fluorophores can be classified into various categories based on structural features including the alkylation of amidogens, the substitution of the O atom of xanthene, and hybridization with other fluorophores.

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Section: Classical Rhodamine Dyesmentioning

confidence: 99%

Hybrid Rhodamine Fluorophores in the Visible/NIR Region for Biological Imaging

et al. 2019

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“…by 10% for Rhodamine 6G for DT = 40°C [13]), causing it to relax non-radiatively to S 0 via internal conversion. It is proposed that in this manner the fluorescence is quenched [14,15].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence depletion mechanisms in super-resolving STED microscopy

Rittweger

Rankin

Westphal

et al. 2007

Chemical Physics Letters

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We prove that stimulated emission is by far the dominant quenching mechanism for providing super-resolution in fluorescence microscopy with a red-shifted depletion beam. Our evidences are based on simultaneously measuring fluorescence quenching and photon gain in the quenching beam. Measurements were performed for several fluorescent dyes including fluorescent proteins over a wide spectral range of their emission spectra. We found that, for each fluorophore, the wavelength dependence of both signals closely follows that of the stimulated emission cross-section.

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“…Xanthene dyes typically exist in solution in a variety of neutral and ionic forms, and several can interact to form dimers. The equilibria among these forms that are sensitive to temperature, pH, solvent, concentration and other factors (4)(5)(6)(7)(8)(9) A number of earlier reports have examined the influence of the solvent environment on xanthene dye photophysics, but there remains considerable scatter in the reported lifetimes and fluorescence efficiencies, and no broad-based systematic study appears to have been carried out. In this report we present a systematic examination of the influence of solvent environment on the fluorescence lifetimes of five xanthene dye species: the rhodamine B zwitterion (RB')?, the rhodamine dye cation (RB+), the rhodamine 6G cation (R6G+), the rhodamine 101 zwitterion (R101') and the fluorescein dianion (F-).…”

Section: Introductionmentioning

confidence: 99%

Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes

Magde

Rojas

Seybold

1999

Photochem & Photobiology

479

254

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Fluorescence lifetimes of Ave representative xanthene dye species‐the rhodamine B zwitterion (RB=), the rhoda‐mine B cation (RB+), the rhodamine 6G cation (R6G+), the rhodamine 101 zwitterion (R101) and the fluorescein dianion (F2‐)‐were measured in H2O, D2O and in a series of alcohol solvents ranging from methanol to octanol. The lifetimes of both RB= and RB+ increased markedly as the solvent was varied from water to octanol. In contrast, the lifetimes of R6G+ and R101± decreased slightly over the alcohol series and that of F2‐ increased only slightly in the same series. For all the dyes studied the fluorescence lifetimes observed in D2O were slightly longer than those in H2O. Possible causes for the variations observed are discussed.

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Temperature-Dependent Measurements of a Flashlamp-Pumped Dye Laser

Abstract: A decreased laser threshold has been observed for a flashlamp-pumped Rhodamine B dye laser as the temperature of the solution is reduced. The change in threshold is attributed to an increase in the fluorescence quantum yield and can be characterized by an activation energy of 880 cm−1.

Cited by 16 publications

References 16 publications

Hybrid Rhodamine Fluorophores in the Visible/NIR Region for Biological Imaging

Hybrid Rhodamine Fluorophores in the Visible/NIR Region for Biological Imaging

Fluorescence depletion mechanisms in super-resolving STED microscopy

Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes

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