Temporal evaluation of the anti‐tumor efficiency of plasma‐activated media

Mohades, Soheila; Barekzi, Nazir; Razavi, Hamid; Maruthamuthu, Venkat; Laroussi, Mounir

doi:10.1002/ppap.201600118

Cited by 42 publications

(41 citation statements)

References 39 publications

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“…The media used to make PAM were MEM (minimum essential media) for SCaBER and Eagle Minimum Essential Media (EMEM) for MDCK. Figure 7 shows the results [57]. Figure 7.…”

Section: Two Biomedical Applications Of Ltpmentioning

confidence: 99%

“…The media used to make PAM were MEM (minimum essential media) for SCaBER and Eagle Minimum Essential Media (EMEM) for MDCK. Figure 7 shows the results [57]. Figure 7 shows the selective effect of LTP when it comes to destroying cancer cells versus healthy cells in vitro.…”

Section: Two Biomedical Applications Of Ltpmentioning

confidence: 99%

“…In cancer cells, the mechanisms of action of LTP are suspected to be related to an increase of intracellular reactive oxygen species (ROS), which can lead to cell cycle arrest at the S-phase, DNA double-strand breaks, and induction of apoptosis. Research by various groups showed that RONS generated by LTP react with cell membranes and can even penetrate the cells and induce subsequent reactions within the cells that can trigger cell-signaling cascades, which can ultimately lead to apoptosis in cancer cells [56][57][58][59][60][61][62][63][64][65][66]. In addition, investigators have shown that plasma-generated RONS can indeed penetrate biological tissues up to depths of more than 1 mm and therefore interact not only with the cells on the surface but with those underneath [67][68][69][70][71][72].…”

Section: Mechanisms Of Biological Action Of Ltp: Brief Summarymentioning

confidence: 99%

“…In addition to direct plasma applications to cancer cells and tissues, investigators reported that plasma-activated media (PAM) can also be used to destroy cancer cells [38,50,[54][55][56][57][58]. Plasma-activated medium is produced by exposing a biological liquid medium to LTP for a length of time (minutes).…”

Section: Ltp Takes On Hygiene and Medical Challengesmentioning

confidence: 99%

“…Viability in percent of SCaBER (cancerous) and MDCK cells (noncancerous) treated by PAM for various lengths of time. Viability was assessed after 12 hours incubation with PAM using MTS assay and Trypan-blue exclusion assay [57]. Figure 7.…”

Section: Two Biomedical Applications Of Ltpmentioning

confidence: 99%

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Plasma Medicine: A Brief Introduction

Laroussi

2018

Plasma

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This mini review is to introduce the readers of Plasma to the field of plasma medicine. This is a multidisciplinary field of research at the intersection of physics, engineering, biology and medicine. Plasma medicine is only about two decades old, but the research community active in this emerging field has grown tremendously in the last few years. Today, research is being conducted on a number of applications including wound healing and cancer treatment. Although a lot of knowledge has been created and our understanding of the fundamental mechanisms that play important roles in the interaction between low temperature plasma and biological cells and tissues has greatly expanded, much remains to be done to get a thorough and detailed picture of all the physical and biochemical processes that enter into play.

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“…The media used to make PAM were MEM (minimum essential media) for SCaBER and Eagle Minimum Essential Media (EMEM) for MDCK. Figure 7 shows the results [57]. Figure 7.…”

Section: Two Biomedical Applications Of Ltpmentioning

confidence: 99%

Section: Two Biomedical Applications Of Ltpmentioning

confidence: 99%

Section: Mechanisms Of Biological Action Of Ltp: Brief Summarymentioning

confidence: 99%

Section: Ltp Takes On Hygiene and Medical Challengesmentioning

confidence: 99%

Section: Two Biomedical Applications Of Ltpmentioning

confidence: 99%

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Plasma Medicine: A Brief Introduction

Laroussi

2018

Plasma

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179

122

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Quantitative assessment of cold atmospheric plasma anti‐cancer efficacy in triple‐negative breast cancers

Dai

Xiang

et al. 2018

Plasma Processes & Polymers

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Cold atmospheric plasma has emerged as a novel oncotherapeutic approach that has undergone a fast growth in the past few years. However, there is no quantitative assessment of the factors influencing its anti-tumor efficacy and the mechanism driving its activity remains mysterious. Through the use of 18 orthogonal experiments followed by linear model construction, we identified four deterministic parameters, that is, "treatment time," "liquid surface area," "thickness of medium," and "number of cells," which play deterministic roles on the anti-tumor efficacy of plasma. We propose "Reactive Species Diffusion Model" and "Signal Transduction Model" to explain the mechanics determining the activity of plasma treated medium and its celldeath induction efficacy. We, in addition, proposed the use of deterministic parameters in defining plasma dose in the liquid form to more objectively reflect its multi-modality nature. Our study contributes in elucidating plasma activity and efficacy in a quantitative way, which guides other plasma related investigations and accelerates plasma clinical applications as a type of precision oncotherapy. K E Y W O R D S cold atmospheric plasma, plasma activated medium, quantitative modeling, triple-negative breast cancer Xiaoyu Xu and Xiaofeng Dai contributed equally to this study.

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Low‐temperature plasma‐activated medium inhibited invasion and metastasis of melanoma cells via suppressing the Wnt/β‐catenin pathway

Liu

Gao

et al. 2019

Plasma Processes & Polymers

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Low‐temperature plasma (LTP) has shown promising and potential applications in the field of biomedicine. The effects of the plasma‐activated medium (PAM) inhibiting tumor cell invasion and metastasis have been verified, but the associated molecular mechanisms are poorly understood. The aim of this study was to identify the anti‐invasion and antimetastasis effects and mechanism induced by PAM on B16 melanoma cells cultured in vitro. For this purpose, an atmospheric pressure plasma jet in helium was applied to generate PAM to treat B16 cells. Cell invasion and migration assays showed that PAM exerted an inhibitory effect on B16 cell invasion and migration in a time‐dependent manner. Then, detection of the invasion‐ and metastasis‐related proteins, including matrix metalloproteinase (MMP)‐2, MMP‐9, CD44, and E‐cadherin, showed that their changes accorded with the invasion and metastasis of the PAM‐treated B16 cells. Furthermore, the Wnt/β‐catenin pathway‐related proteins in PAM‐treated cells were measured, and the pathway was found to be suppressed by PAM. Finally, numerous specific reactive oxygen species (ROS) scavengers were applied to eliminate LTP‐induced ROS in PAM, and it was revealed that H2O2 played a critical role in these processes. These findings demonstrated that PAM exerted anti‐invasion and antimetastasis effects on B16 cells mainly by suppressing the Wnt/β‐catenin pathway, which consequently decreased the expressions of MMP‐9, MMP‐2, and CD44 via H2O2.

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Temporal evaluation of the anti‐tumor efficiency of plasma‐activated media

Cited by 42 publications

References 39 publications

Plasma Medicine: A Brief Introduction

Plasma Medicine: A Brief Introduction

Quantitative assessment of cold atmospheric plasma anti‐cancer efficacy in triple‐negative breast cancers

Low‐temperature plasma‐activated medium inhibited invasion and metastasis of melanoma cells via suppressing the Wnt/β‐catenin pathway

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