Temporal variation of chemical composition and relaxant action of the essential oil of Ocimum gratissimum L. (Labiatae) on guinea-pig ileum

Madeira, Socorro Vanesca Frota; Rabelo, M. D.; Soares, Pedro Marcos Gomes; Souza, Emmanuel Prata de; Meireles, Ana Vanesca P.; Montenegro, C.; Lima, Ricardo; Assreuy, Ana Maria Sampaio; Criddle, David N.

doi:10.1016/j.phymed.2003.11.009

Cited by 21 publications

(12 citation statements)

References 8 publications

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“…EOOG 8 and EOOG 12 relaxed 60 mM KClprecontracted preparations similarly (38.33±9.91 μg/ml and 35.53±6.70), whereas a significantly more potent relaxant effect of EOOG 12 compared to EOOG 8 was observed when tissues were contracted using 10 μM acetylcholine The principal constituents of the essential oil, eugenol and cineole, also relaxed KCl-precontracted preparations, although they were less potent than EOOG. Results showed that the essential oil extracted from the leaves of O. gratissimum, collected at different time periods, exerts significant relaxant effects on isolated guinea-pig ileum which may underlie the therapeutic action of the plant [51].…”

Section: Gastro Intestinal Tractmentioning

confidence: 99%

Ocimum gratissimum: A Review of its Chemical, Pharmacological and Ethnomedicinal Properties

Prabhu¹,

Lobo²,

Shirwaikar³

2009

TOALTMEDJ

131

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Abstract:Ocimum gratissimum is a well-known plant used in the Indian system of medicine. Folklore medicine claims its use in headache, fever, diarrhoea, pneumonia etc. Research carried out using different in vitro and in vivo techniques of biological evaluation supports most of the claims. This review presents the ethnobotanical, natural product chemistry, pharmacological, clinical and toxicological data of the plant.

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Section: Gastro Intestinal Tractmentioning

confidence: 99%

Ocimum gratissimum: A Review of its Chemical, Pharmacological and Ethnomedicinal Properties

Prabhu¹,

Lobo²,

Shirwaikar³

2009

TOALTMEDJ

131

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“…gratissimum (EOOG) and its main constituent eugenol (EUG). It has been shown that EOOG exerts antispasmodic effects on guinea‐pig isolated ileum, justifying the use of the plant in folk medicine for the treatment of gastrointestinal disorders [4,5]. Such a myorelaxant activity has also been demonstrated for EUG in rat isolated ileum, suggesting that the antispasmodic activity of EOOG could be attributed, in part, to the actions of this constituent [6].…”

Section: Introductionmentioning

confidence: 99%

Pharmacological evidence of calcium‐channel blockade by essential oil of Ocimum gratissimum and its main constituent, eugenol, in isolated aortic rings from DOCA‐salt hypertensive rats

Interaminense

Jucá

Magalhães

et al. 2007

Fundamemntal Clinical Pharma

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Intravenous (i.v.) treatment of conscious DOCA-salt hypertensive rats with the essential oil of Ocimum gratissimum L. (Labiatae) (EOOG) induced a hypotensive effect that seems related to an active vascular relaxation rather than withdrawal of sympathetic tone. To corroborate this hypothesis, the present study examined the vascular effects of EOOG and its main constituent, eugenol (EUG) and the putative mechanisms underlying these effects. Additionally, the role of the vascular beta(2)-adrenergic mechanism in the mediation of EOOG-induced hypotension has also been investigated. In conscious DOCA-salt hypertensive rats, the EOOG-induced hypotension was reversible and remained unchanged by i.v. pretreatment with propranolol (2 mg/kg). In isolated aorta preparations with intact endothelium from DOCA-salt hypertensive rats, EOOG (1-1000 microg/mL) and EUG (0.006-6 mM) relaxed the phenylephrine-induced contraction similarly with IC(50) [geometric mean (95% confidence interval)] values of 226.9 (147.8-348.3) microg/mL and 1.2 (0.6-2.1) mm, respectively. Vasorelaxant effects of EOOG were significantly altered by removal of the vascular endothelium [IC(50) = 417.2 (349.5-497.8) microg/mL]. In a calcium-free medium, the CaCl(2)-induced contractions were significantly reduced and even abolished by EOOG at 300 and 1000 microg/mL, respectively, whereas EOOG (1000 microg/mL) did not have any significant effect on caffeine-induced contractions. Similar results were obtained with EUG (1.8 and 6 mM) on both CaCl(2)- and caffeine-induced contractions, respectively. The data suggest that hypotensive responses to EOOG in DOCA-salt hypertensive rats are due to an active vascular relaxation, which is partly dependent upon the integrity of the vascular endothelium and seems predominantly mediated through an inhibition of plasmalemmal Ca(2+) influx rather than Ca(2+)-induced Ca(2+) release from the sarcoplasmic reticulum.

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“…This spasmolytic activity of EOAH may be due to the presence of camphor, terpinene [33], 1,8-cineol [34], α- and β-pinene [35], which have been reported to be smooth muscle relaxants. However, the presence of other spasmolytic compound(s) cannot be excluded.…”

Section: Resultsmentioning

confidence: 99%

Relaxant Effect of Essential Oil of Artemisia herba-alba Asso. on Rodent Jejunum Contractions

Aziz¹,

Karim²,

M³

et al. 2012

Sci. Pharm.

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Artemisia herba-alba Asso. is a shrub commonly encountered in Morocco. It is used in traditional medicine for treating intestinal disorders. The essential oil extracted from the plant’s aerial parts reversibly relaxed the spontaneous tonus of the rabbit jejunum in a reversible concentration dependent manner with an IC50 value of 97.33 ± 2.59 ng/ml and reversed the tonic contraction of rat jejunum induced by 75 mM KCl and 10−6 M carbachol with IC50 values of 115.5 ± 3.05 and 119.4 ± 20.86 ng/ml, respectively. The pre-treatment of the latter isolated intestine with this essential oil produced a dose-dependent shift of the Ca++ and CCh dose-response curve to the right, with suppression of the maximal effect, similar to the non-competitive antagonist effect on muscarinic receptors and calcium channel, respectively.

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Temporal variation of chemical composition and relaxant action of the essential oil of Ocimum gratissimum L. (Labiatae) on guinea-pig ileum

Cited by 21 publications

References 8 publications

Ocimum gratissimum: A Review of its Chemical, Pharmacological and Ethnomedicinal Properties

Ocimum gratissimum: A Review of its Chemical, Pharmacological and Ethnomedicinal Properties

Pharmacological evidence of calcium‐channel blockade by essential oil of Ocimum gratissimum and its main constituent, eugenol, in isolated aortic rings from DOCA‐salt hypertensive rats

Relaxant Effect of Essential Oil of Artemisia herba-alba Asso. on Rodent Jejunum Contractions

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